Scale‐up of pseudo solid‐phase enzymatic synthesis of α‐methyl glucoside acrylate
Abstract The successful scale‐up of the enzymatic synthesis of α‐methyl glucoside acrylate from laboratory‐scale (milliliter) to pilot‐scale (liter) was examined. Specifically, Candida antarctica lipase B (Novozym 435) was used as a biocatalyst to produce α‐methyl glucoside acrylate via the transest...
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crwiley:10.1002/bit.10272 2024-06-23T07:47:59+00:00 Scale‐up of pseudo solid‐phase enzymatic synthesis of α‐methyl glucoside acrylate Li, Yanzi Rethwisch, David G. 2002 http://dx.doi.org/10.1002/bit.10272 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fbit.10272 https://onlinelibrary.wiley.com/doi/pdf/10.1002/bit.10272 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Biotechnology and Bioengineering volume 79, issue 1, page 15-22 ISSN 0006-3592 1097-0290 journal-article 2002 crwiley https://doi.org/10.1002/bit.10272 2024-06-11T04:49:20Z Abstract The successful scale‐up of the enzymatic synthesis of α‐methyl glucoside acrylate from laboratory‐scale (milliliter) to pilot‐scale (liter) was examined. Specifically, Candida antarctica lipase B (Novozym 435) was used as a biocatalyst to produce α‐methyl glucoside acrylate via the transesterification of α‐methyl glucoside (MG) with vinyl acrylate (VA) using acetone as a solvent. This is a pseudo‐solid‐phase synthesis; only a fraction of the α‐methyl glucoside and the product are soluble in acetone. Molecular sieves were used to remove traces of water in the reaction medium and to increase enzyme stability by removing the acetaldehyde by‐product. A general method was also developed to purify and recover the monoacrylate product from unreacted sugar and undesired diester by a simple crystallization and precipitation process. © 2002 Wiley Periodicals, Inc. Biotechnol Bioeng 79: 15–22, 2002. Article in Journal/Newspaper Antarc* Antarctica Wiley Online Library Biotechnology and Bioengineering 79 1 15 22 |
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English |
description |
Abstract The successful scale‐up of the enzymatic synthesis of α‐methyl glucoside acrylate from laboratory‐scale (milliliter) to pilot‐scale (liter) was examined. Specifically, Candida antarctica lipase B (Novozym 435) was used as a biocatalyst to produce α‐methyl glucoside acrylate via the transesterification of α‐methyl glucoside (MG) with vinyl acrylate (VA) using acetone as a solvent. This is a pseudo‐solid‐phase synthesis; only a fraction of the α‐methyl glucoside and the product are soluble in acetone. Molecular sieves were used to remove traces of water in the reaction medium and to increase enzyme stability by removing the acetaldehyde by‐product. A general method was also developed to purify and recover the monoacrylate product from unreacted sugar and undesired diester by a simple crystallization and precipitation process. © 2002 Wiley Periodicals, Inc. Biotechnol Bioeng 79: 15–22, 2002. |
format |
Article in Journal/Newspaper |
author |
Li, Yanzi Rethwisch, David G. |
spellingShingle |
Li, Yanzi Rethwisch, David G. Scale‐up of pseudo solid‐phase enzymatic synthesis of α‐methyl glucoside acrylate |
author_facet |
Li, Yanzi Rethwisch, David G. |
author_sort |
Li, Yanzi |
title |
Scale‐up of pseudo solid‐phase enzymatic synthesis of α‐methyl glucoside acrylate |
title_short |
Scale‐up of pseudo solid‐phase enzymatic synthesis of α‐methyl glucoside acrylate |
title_full |
Scale‐up of pseudo solid‐phase enzymatic synthesis of α‐methyl glucoside acrylate |
title_fullStr |
Scale‐up of pseudo solid‐phase enzymatic synthesis of α‐methyl glucoside acrylate |
title_full_unstemmed |
Scale‐up of pseudo solid‐phase enzymatic synthesis of α‐methyl glucoside acrylate |
title_sort |
scale‐up of pseudo solid‐phase enzymatic synthesis of α‐methyl glucoside acrylate |
publisher |
Wiley |
publishDate |
2002 |
url |
http://dx.doi.org/10.1002/bit.10272 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fbit.10272 https://onlinelibrary.wiley.com/doi/pdf/10.1002/bit.10272 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Biotechnology and Bioengineering volume 79, issue 1, page 15-22 ISSN 0006-3592 1097-0290 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/bit.10272 |
container_title |
Biotechnology and Bioengineering |
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79 |
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1 |
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15 |
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22 |
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1802638308911611904 |