Full model for reversible kinetics of lipase‐catalyzed sugar–ester synthesis in 2‐methyl 2‐butanol
Abstract A kinetic model derived from the ping‐pong bi‐bi reversible mechanism is proposed to described the acylation of glucose by lauric acid in 2‐methyl 2‐butanol mediated by Candida antarctica lipase at 60°C. The model accounts for the effect of all four compounds in the reaction mixture, namely...
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crwiley:10.1002/bit.10260 2024-06-02T07:58:17+00:00 Full model for reversible kinetics of lipase‐catalyzed sugar–ester synthesis in 2‐methyl 2‐butanol Flores, Maria Victoria Halling, Peter J. 2002 http://dx.doi.org/10.1002/bit.10260 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fbit.10260 https://onlinelibrary.wiley.com/doi/pdf/10.1002/bit.10260 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Biotechnology and Bioengineering volume 78, issue 7, page 795-801 ISSN 0006-3592 1097-0290 journal-article 2002 crwiley https://doi.org/10.1002/bit.10260 2024-05-03T10:50:33Z Abstract A kinetic model derived from the ping‐pong bi‐bi reversible mechanism is proposed to described the acylation of glucose by lauric acid in 2‐methyl 2‐butanol mediated by Candida antarctica lipase at 60°C. The model accounts for the effect of all four compounds in the reaction mixture, namely lauric acid, glucose, water, and lauroyl glucose ester. A supersaturated glucose solution was used to avoid limitations by glucose dissolution rate. Experiments with varied initial water content were performed to determine the effect of water on the initial reaction rate. The full time course of ester formation is described by five parameters: (a) three parameters evaluated from initial rate measurements; (b) the equilibrium constant, independently evaluated; and (c) one extra parameter fitted to the progress curve of ester formation. This reduced form of a full reversible kinetic model based on the ping‐pong bi‐bi mechanism is able to describe the complete course of lauroyl glucose ester synthesis. The proposed model provides a good fit for the experimental results. © 2002 Wiley Periodicals, Inc. Biotechnol Bioeng 78: 795–801, 2002. Article in Journal/Newspaper Antarc* Antarctica Wiley Online Library Biotechnology and Bioengineering 78 7 795 801 |
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English |
description |
Abstract A kinetic model derived from the ping‐pong bi‐bi reversible mechanism is proposed to described the acylation of glucose by lauric acid in 2‐methyl 2‐butanol mediated by Candida antarctica lipase at 60°C. The model accounts for the effect of all four compounds in the reaction mixture, namely lauric acid, glucose, water, and lauroyl glucose ester. A supersaturated glucose solution was used to avoid limitations by glucose dissolution rate. Experiments with varied initial water content were performed to determine the effect of water on the initial reaction rate. The full time course of ester formation is described by five parameters: (a) three parameters evaluated from initial rate measurements; (b) the equilibrium constant, independently evaluated; and (c) one extra parameter fitted to the progress curve of ester formation. This reduced form of a full reversible kinetic model based on the ping‐pong bi‐bi mechanism is able to describe the complete course of lauroyl glucose ester synthesis. The proposed model provides a good fit for the experimental results. © 2002 Wiley Periodicals, Inc. Biotechnol Bioeng 78: 795–801, 2002. |
format |
Article in Journal/Newspaper |
author |
Flores, Maria Victoria Halling, Peter J. |
spellingShingle |
Flores, Maria Victoria Halling, Peter J. Full model for reversible kinetics of lipase‐catalyzed sugar–ester synthesis in 2‐methyl 2‐butanol |
author_facet |
Flores, Maria Victoria Halling, Peter J. |
author_sort |
Flores, Maria Victoria |
title |
Full model for reversible kinetics of lipase‐catalyzed sugar–ester synthesis in 2‐methyl 2‐butanol |
title_short |
Full model for reversible kinetics of lipase‐catalyzed sugar–ester synthesis in 2‐methyl 2‐butanol |
title_full |
Full model for reversible kinetics of lipase‐catalyzed sugar–ester synthesis in 2‐methyl 2‐butanol |
title_fullStr |
Full model for reversible kinetics of lipase‐catalyzed sugar–ester synthesis in 2‐methyl 2‐butanol |
title_full_unstemmed |
Full model for reversible kinetics of lipase‐catalyzed sugar–ester synthesis in 2‐methyl 2‐butanol |
title_sort |
full model for reversible kinetics of lipase‐catalyzed sugar–ester synthesis in 2‐methyl 2‐butanol |
publisher |
Wiley |
publishDate |
2002 |
url |
http://dx.doi.org/10.1002/bit.10260 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fbit.10260 https://onlinelibrary.wiley.com/doi/pdf/10.1002/bit.10260 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Biotechnology and Bioengineering volume 78, issue 7, page 795-801 ISSN 0006-3592 1097-0290 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/bit.10260 |
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Biotechnology and Bioengineering |
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78 |
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7 |
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795 |
op_container_end_page |
801 |
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1800741574562283520 |