Effect of water activity and immobilization on fatty acid selectivity for esterification reactions mediated by lipases
Abstract The effect of water activity (a w ) and immobilization on fatty acid (FA) selectivity of Burkholderia (formerly Pseudomonas ) cepacia, Rhizomucor miehei, Candida antarctica (type B), and Candida rugosa lipases in esterification reactions was determined. Studies were based on measuring ester...
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crwiley:10.1002/bit.10009 2024-06-02T07:57:42+00:00 Effect of water activity and immobilization on fatty acid selectivity for esterification reactions mediated by lipases Lee, Chen‐Hsien Parkin, Kirk L. 2001 http://dx.doi.org/10.1002/bit.10009 https://onlinelibrary.wiley.com/doi/pdf/10.1002/bit.10009 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Biotechnology and Bioengineering volume 75, issue 2, page 219-227 ISSN 0006-3592 1097-0290 journal-article 2001 crwiley https://doi.org/10.1002/bit.10009 2024-05-03T12:01:08Z Abstract The effect of water activity (a w ) and immobilization on fatty acid (FA) selectivity of Burkholderia (formerly Pseudomonas ) cepacia, Rhizomucor miehei, Candida antarctica (type B), and Candida rugosa lipases in esterification reactions was determined. Studies were based on measuring ester formation in multicompetitive reaction mixtures containing either the homologous series of even carbon number n ‐chain saturated FA (C4‐C18) or a series of n ‐chain (un)saturated FA (C18:X, where X = 0–3 double bonds) as cosubstrates with 1,3‐propanediol in ter ‐butyl methyl ether at a w of 0.19, 0.69, and 0.90. Activity and FA selectively patterns were similar for free and Celite‐adsorbed lipases in response to changes in a w , although specific effects were observed for selectivity of B. cepacia and C. rugosa lipases toward C16 and C4/C6 FA, respectively. Also, selectivity toward unsaturated C18:X FA as a group was modulated by changes in a w for three of the four lipase studied. Resin‐fixed lipases from R. miehei and C. antarctica exhibited profound differences in activity and FA selectively in response to changes in a w , relative to free and Celite‐bound forms. These findings suggest that FA selectivity for lipid modification is influenced by a w and immobilization, but that each lipase has a characteristic response to these factors in a manner that cannot be predicted. © 2001 John Wiley & Sons, Inc. Biotechnol Bioeng 75: 219–227, 2001. Article in Journal/Newspaper Antarc* Antarctica Wiley Online Library Rugosa ENVELOPE(-61.250,-61.250,-62.633,-62.633) Biotechnology and Bioengineering 75 2 219 227 |
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English |
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Abstract The effect of water activity (a w ) and immobilization on fatty acid (FA) selectivity of Burkholderia (formerly Pseudomonas ) cepacia, Rhizomucor miehei, Candida antarctica (type B), and Candida rugosa lipases in esterification reactions was determined. Studies were based on measuring ester formation in multicompetitive reaction mixtures containing either the homologous series of even carbon number n ‐chain saturated FA (C4‐C18) or a series of n ‐chain (un)saturated FA (C18:X, where X = 0–3 double bonds) as cosubstrates with 1,3‐propanediol in ter ‐butyl methyl ether at a w of 0.19, 0.69, and 0.90. Activity and FA selectively patterns were similar for free and Celite‐adsorbed lipases in response to changes in a w , although specific effects were observed for selectivity of B. cepacia and C. rugosa lipases toward C16 and C4/C6 FA, respectively. Also, selectivity toward unsaturated C18:X FA as a group was modulated by changes in a w for three of the four lipase studied. Resin‐fixed lipases from R. miehei and C. antarctica exhibited profound differences in activity and FA selectively in response to changes in a w , relative to free and Celite‐bound forms. These findings suggest that FA selectivity for lipid modification is influenced by a w and immobilization, but that each lipase has a characteristic response to these factors in a manner that cannot be predicted. © 2001 John Wiley & Sons, Inc. Biotechnol Bioeng 75: 219–227, 2001. |
format |
Article in Journal/Newspaper |
author |
Lee, Chen‐Hsien Parkin, Kirk L. |
spellingShingle |
Lee, Chen‐Hsien Parkin, Kirk L. Effect of water activity and immobilization on fatty acid selectivity for esterification reactions mediated by lipases |
author_facet |
Lee, Chen‐Hsien Parkin, Kirk L. |
author_sort |
Lee, Chen‐Hsien |
title |
Effect of water activity and immobilization on fatty acid selectivity for esterification reactions mediated by lipases |
title_short |
Effect of water activity and immobilization on fatty acid selectivity for esterification reactions mediated by lipases |
title_full |
Effect of water activity and immobilization on fatty acid selectivity for esterification reactions mediated by lipases |
title_fullStr |
Effect of water activity and immobilization on fatty acid selectivity for esterification reactions mediated by lipases |
title_full_unstemmed |
Effect of water activity and immobilization on fatty acid selectivity for esterification reactions mediated by lipases |
title_sort |
effect of water activity and immobilization on fatty acid selectivity for esterification reactions mediated by lipases |
publisher |
Wiley |
publishDate |
2001 |
url |
http://dx.doi.org/10.1002/bit.10009 https://onlinelibrary.wiley.com/doi/pdf/10.1002/bit.10009 |
long_lat |
ENVELOPE(-61.250,-61.250,-62.633,-62.633) |
geographic |
Rugosa |
geographic_facet |
Rugosa |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Biotechnology and Bioengineering volume 75, issue 2, page 219-227 ISSN 0006-3592 1097-0290 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/bit.10009 |
container_title |
Biotechnology and Bioengineering |
container_volume |
75 |
container_issue |
2 |
container_start_page |
219 |
op_container_end_page |
227 |
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1800740892609347584 |