Observation of small conformational changes in the sperm‐whale myoglobin by the far‐infrared spectra
Abstract Small conformational changes in a molecule of sperm‐whale myoglobin in its native solid state for different pH values at room temperature as well as during heat denaturation in alkali medium at different stages of unfolding of the globule were observed by using far‐infrared spectroscopy in...
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Online Access: | http://dx.doi.org/10.1002/bip.1973.360120315 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fbip.1973.360120315 https://onlinelibrary.wiley.com/doi/pdf/10.1002/bip.1973.360120315 |
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crwiley:10.1002/bip.1973.360120315 2024-06-02T08:14:52+00:00 Observation of small conformational changes in the sperm‐whale myoglobin by the far‐infrared spectra Chirgadze, Yu. N. Ovsepyan, A. M. 1973 http://dx.doi.org/10.1002/bip.1973.360120315 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fbip.1973.360120315 https://onlinelibrary.wiley.com/doi/pdf/10.1002/bip.1973.360120315 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Biopolymers volume 12, issue 3, page 637-645 ISSN 0006-3525 1097-0282 journal-article 1973 crwiley https://doi.org/10.1002/bip.1973.360120315 2024-05-03T11:45:47Z Abstract Small conformational changes in a molecule of sperm‐whale myoglobin in its native solid state for different pH values at room temperature as well as during heat denaturation in alkali medium at different stages of unfolding of the globule were observed by using far‐infrared spectroscopy in the region from 30 to 600 cm −1 . The changes appeared in the absorption bands near 420 and 470 cm −1 ascribed to the side‐chain vibrations of helical segments of the myoglobin molecule. For the first time the high structural sensitivity of the far‐infrared region of the skeletal vibrations has been confirmed experimentally and the applicability of this technique to globular proteins demonstrated. Article in Journal/Newspaper Sperm whale Wiley Online Library Biopolymers 12 3 637 645 |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Abstract Small conformational changes in a molecule of sperm‐whale myoglobin in its native solid state for different pH values at room temperature as well as during heat denaturation in alkali medium at different stages of unfolding of the globule were observed by using far‐infrared spectroscopy in the region from 30 to 600 cm −1 . The changes appeared in the absorption bands near 420 and 470 cm −1 ascribed to the side‐chain vibrations of helical segments of the myoglobin molecule. For the first time the high structural sensitivity of the far‐infrared region of the skeletal vibrations has been confirmed experimentally and the applicability of this technique to globular proteins demonstrated. |
format |
Article in Journal/Newspaper |
author |
Chirgadze, Yu. N. Ovsepyan, A. M. |
spellingShingle |
Chirgadze, Yu. N. Ovsepyan, A. M. Observation of small conformational changes in the sperm‐whale myoglobin by the far‐infrared spectra |
author_facet |
Chirgadze, Yu. N. Ovsepyan, A. M. |
author_sort |
Chirgadze, Yu. N. |
title |
Observation of small conformational changes in the sperm‐whale myoglobin by the far‐infrared spectra |
title_short |
Observation of small conformational changes in the sperm‐whale myoglobin by the far‐infrared spectra |
title_full |
Observation of small conformational changes in the sperm‐whale myoglobin by the far‐infrared spectra |
title_fullStr |
Observation of small conformational changes in the sperm‐whale myoglobin by the far‐infrared spectra |
title_full_unstemmed |
Observation of small conformational changes in the sperm‐whale myoglobin by the far‐infrared spectra |
title_sort |
observation of small conformational changes in the sperm‐whale myoglobin by the far‐infrared spectra |
publisher |
Wiley |
publishDate |
1973 |
url |
http://dx.doi.org/10.1002/bip.1973.360120315 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fbip.1973.360120315 https://onlinelibrary.wiley.com/doi/pdf/10.1002/bip.1973.360120315 |
genre |
Sperm whale |
genre_facet |
Sperm whale |
op_source |
Biopolymers volume 12, issue 3, page 637-645 ISSN 0006-3525 1097-0282 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/bip.1973.360120315 |
container_title |
Biopolymers |
container_volume |
12 |
container_issue |
3 |
container_start_page |
637 |
op_container_end_page |
645 |
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1800738860180701184 |