Lipase‐catalyzed dimethyl adipate synthesis: Response surface modeling and kinetics
Abstract Dimethyl adipate (DMA) was synthesized by immobilized Candida antarctica lipase B‐catalyzed esterification of adipic acid and methanol. To optimize the reaction conditions of ester production, response surface methodology was applied, and the effects of four factors namely, time, temperatur...
Published in: | Biotechnology Journal |
---|---|
Main Authors: | , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Wiley
2010
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1002/biot.201000063 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fbiot.201000063 https://onlinelibrary.wiley.com/doi/pdf/10.1002/biot.201000063 |
id |
crwiley:10.1002/biot.201000063 |
---|---|
record_format |
openpolar |
spelling |
crwiley:10.1002/biot.201000063 2024-09-09T19:09:31+00:00 Lipase‐catalyzed dimethyl adipate synthesis: Response surface modeling and kinetics Chaibakhsh, Naz Rahman, Mohd Basyaruddin Abdul Basri, Mahiran Salleh, Abu Bakar Abd‐Aziz, Suraini 2010 http://dx.doi.org/10.1002/biot.201000063 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fbiot.201000063 https://onlinelibrary.wiley.com/doi/pdf/10.1002/biot.201000063 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Biotechnology Journal volume 5, issue 8, page 848-855 ISSN 1860-6768 1860-7314 journal-article 2010 crwiley https://doi.org/10.1002/biot.201000063 2024-06-20T04:26:58Z Abstract Dimethyl adipate (DMA) was synthesized by immobilized Candida antarctica lipase B‐catalyzed esterification of adipic acid and methanol. To optimize the reaction conditions of ester production, response surface methodology was applied, and the effects of four factors namely, time, temperature, enzyme concentration, and molar ratio of substrates on product synthesis were determined. A statistical model predicted that the maximum conversion yield would be 97.6%, at the optimal conditions of 58.5°C, 54.0 mg enzyme, 358.0 min, and 12:1 molar ratio of methanol to adipic acid. The R 2 (0.9769) shows a high correlation between predicted and experimental values. The kinetics of the reaction was also investigated in this study. The reaction was found to obey the ping‐pong bi‐bi mechanism with methanol inhibition. The kinetic parameters were determined and used to simulate the experimental results. A good quality of fit was observed between the simulated and experimental initial rates. Article in Journal/Newspaper Antarc* Antarctica Wiley Online Library Biotechnology Journal 5 8 848 855 |
institution |
Open Polar |
collection |
Wiley Online Library |
op_collection_id |
crwiley |
language |
English |
description |
Abstract Dimethyl adipate (DMA) was synthesized by immobilized Candida antarctica lipase B‐catalyzed esterification of adipic acid and methanol. To optimize the reaction conditions of ester production, response surface methodology was applied, and the effects of four factors namely, time, temperature, enzyme concentration, and molar ratio of substrates on product synthesis were determined. A statistical model predicted that the maximum conversion yield would be 97.6%, at the optimal conditions of 58.5°C, 54.0 mg enzyme, 358.0 min, and 12:1 molar ratio of methanol to adipic acid. The R 2 (0.9769) shows a high correlation between predicted and experimental values. The kinetics of the reaction was also investigated in this study. The reaction was found to obey the ping‐pong bi‐bi mechanism with methanol inhibition. The kinetic parameters were determined and used to simulate the experimental results. A good quality of fit was observed between the simulated and experimental initial rates. |
format |
Article in Journal/Newspaper |
author |
Chaibakhsh, Naz Rahman, Mohd Basyaruddin Abdul Basri, Mahiran Salleh, Abu Bakar Abd‐Aziz, Suraini |
spellingShingle |
Chaibakhsh, Naz Rahman, Mohd Basyaruddin Abdul Basri, Mahiran Salleh, Abu Bakar Abd‐Aziz, Suraini Lipase‐catalyzed dimethyl adipate synthesis: Response surface modeling and kinetics |
author_facet |
Chaibakhsh, Naz Rahman, Mohd Basyaruddin Abdul Basri, Mahiran Salleh, Abu Bakar Abd‐Aziz, Suraini |
author_sort |
Chaibakhsh, Naz |
title |
Lipase‐catalyzed dimethyl adipate synthesis: Response surface modeling and kinetics |
title_short |
Lipase‐catalyzed dimethyl adipate synthesis: Response surface modeling and kinetics |
title_full |
Lipase‐catalyzed dimethyl adipate synthesis: Response surface modeling and kinetics |
title_fullStr |
Lipase‐catalyzed dimethyl adipate synthesis: Response surface modeling and kinetics |
title_full_unstemmed |
Lipase‐catalyzed dimethyl adipate synthesis: Response surface modeling and kinetics |
title_sort |
lipase‐catalyzed dimethyl adipate synthesis: response surface modeling and kinetics |
publisher |
Wiley |
publishDate |
2010 |
url |
http://dx.doi.org/10.1002/biot.201000063 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fbiot.201000063 https://onlinelibrary.wiley.com/doi/pdf/10.1002/biot.201000063 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Biotechnology Journal volume 5, issue 8, page 848-855 ISSN 1860-6768 1860-7314 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/biot.201000063 |
container_title |
Biotechnology Journal |
container_volume |
5 |
container_issue |
8 |
container_start_page |
848 |
op_container_end_page |
855 |
_version_ |
1809823780458987520 |