Water‐in‐ionic liquid microemulsion‐based organogels as novel matrices for enzyme immobilization

• Published in: Biotechnology Journal
• Main Authors: Pavlidis, Ioannis V., Tzafestas, Kyriakos, Stamatis, Haralambos
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wiley 2010
• Subjects: Antarc*; Antarctica
• Online Access: http://dx.doi.org/10.1002/biot.201000052; https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fbiot.201000052; https://onlinelibrary.wiley.com/doi/pdf/10.1002/biot.201000052

Abstract The use of water‐in‐ionic liquid microemulsion‐based organogels (w/IL MBGs) as novel supports for the immobilization of lipase B from Candida antarctica and lipase from Chromobacterium viscosum was investigated. These novel lipase‐containing w/IL MBGs can be effectively used as solid phase biocatalysts in various polar and non‐polar organic solvents or ILs, exhibiting up to 4.4‐fold higher esterification activity compared to water‐in‐oil microemulsion‐based organogels. The immobilized lipases retain their activity for several hours at 70°C, while their half life time is up to 25‐fold higher compared to that observed in w/IL microemulsions. Fourier‐transform infrared spectroscopy data indicate that immobilized lipases adopt a more rigid structure, referring to the structure in aqueous solution, which is in correlation with their enhanced catalytic behavior observed.