Optimization of peroxynitrite–luminol chemiluminescence system for detecting peroxynitrite in cell culture solution exposed to carbon disulphide
Abstract We established a peroxynitrite–luminol chemiluminescence system for detecting peroxynitrite in cell culture solution exposed to carbon disulphide (CS 2 ). Three factors, including exposure time to ozone (Factor A), volume of peroxynitrite (ONOO − ) solution (Factor B) and luminol concentrat...
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crwiley:10.1002/bio.734 2024-06-09T07:45:20+00:00 Optimization of peroxynitrite–luminol chemiluminescence system for detecting peroxynitrite in cell culture solution exposed to carbon disulphide Chen, Shao L. Jian, Le Lang, Hui Q. 2003 http://dx.doi.org/10.1002/bio.734 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fbio.734 https://onlinelibrary.wiley.com/doi/full/10.1002/bio.734 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Luminescence volume 18, issue 5, page 249-253 ISSN 1522-7235 1522-7243 journal-article 2003 crwiley https://doi.org/10.1002/bio.734 2024-05-16T14:25:50Z Abstract We established a peroxynitrite–luminol chemiluminescence system for detecting peroxynitrite in cell culture solution exposed to carbon disulphide (CS 2 ). Three factors, including exposure time to ozone (Factor A), volume of peroxynitrite (ONOO − ) solution (Factor B) and luminol concentrations (Factor C) at three levels were selected and the combinations were in accordance with orthogonal design L 9 (3 4 ). Peroxynitrite was generated from the reaction of ozone and 0.01 mol/L sodium azide (NaN 3 ) dissolved in carbonic acid buffer solution (pH 11), and it was reacted with luminol to yield chemiluminescence. The peak value, peak time and kinetic curve of the light emission were observed. The selected combination conditions were 50 s ozone, 800 µL peroxynitrite and 0.001 mol/L luminol solution. Cell culture solution with CS 2 enhanced the emission intensity of chemiluminescence ( F = 8.38, p = 0.018) and shortened the peak time to chemiluminescence ( F = 139.00, p = 0.0001). The data demonstrated that this luminol chemiluminescence system is suitable for detecting peroxynitrite in cell culture solutions for evaluating the effect of CS 2 on endothelial cells. Copyright © 2003 John Wiley & Sons, Ltd. Article in Journal/Newspaper Carbonic acid Wiley Online Library Luminescence 18 5 249 253 |
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description |
Abstract We established a peroxynitrite–luminol chemiluminescence system for detecting peroxynitrite in cell culture solution exposed to carbon disulphide (CS 2 ). Three factors, including exposure time to ozone (Factor A), volume of peroxynitrite (ONOO − ) solution (Factor B) and luminol concentrations (Factor C) at three levels were selected and the combinations were in accordance with orthogonal design L 9 (3 4 ). Peroxynitrite was generated from the reaction of ozone and 0.01 mol/L sodium azide (NaN 3 ) dissolved in carbonic acid buffer solution (pH 11), and it was reacted with luminol to yield chemiluminescence. The peak value, peak time and kinetic curve of the light emission were observed. The selected combination conditions were 50 s ozone, 800 µL peroxynitrite and 0.001 mol/L luminol solution. Cell culture solution with CS 2 enhanced the emission intensity of chemiluminescence ( F = 8.38, p = 0.018) and shortened the peak time to chemiluminescence ( F = 139.00, p = 0.0001). The data demonstrated that this luminol chemiluminescence system is suitable for detecting peroxynitrite in cell culture solutions for evaluating the effect of CS 2 on endothelial cells. Copyright © 2003 John Wiley & Sons, Ltd. |
format |
Article in Journal/Newspaper |
author |
Chen, Shao L. Jian, Le Lang, Hui Q. |
spellingShingle |
Chen, Shao L. Jian, Le Lang, Hui Q. Optimization of peroxynitrite–luminol chemiluminescence system for detecting peroxynitrite in cell culture solution exposed to carbon disulphide |
author_facet |
Chen, Shao L. Jian, Le Lang, Hui Q. |
author_sort |
Chen, Shao L. |
title |
Optimization of peroxynitrite–luminol chemiluminescence system for detecting peroxynitrite in cell culture solution exposed to carbon disulphide |
title_short |
Optimization of peroxynitrite–luminol chemiluminescence system for detecting peroxynitrite in cell culture solution exposed to carbon disulphide |
title_full |
Optimization of peroxynitrite–luminol chemiluminescence system for detecting peroxynitrite in cell culture solution exposed to carbon disulphide |
title_fullStr |
Optimization of peroxynitrite–luminol chemiluminescence system for detecting peroxynitrite in cell culture solution exposed to carbon disulphide |
title_full_unstemmed |
Optimization of peroxynitrite–luminol chemiluminescence system for detecting peroxynitrite in cell culture solution exposed to carbon disulphide |
title_sort |
optimization of peroxynitrite–luminol chemiluminescence system for detecting peroxynitrite in cell culture solution exposed to carbon disulphide |
publisher |
Wiley |
publishDate |
2003 |
url |
http://dx.doi.org/10.1002/bio.734 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fbio.734 https://onlinelibrary.wiley.com/doi/full/10.1002/bio.734 |
genre |
Carbonic acid |
genre_facet |
Carbonic acid |
op_source |
Luminescence volume 18, issue 5, page 249-253 ISSN 1522-7235 1522-7243 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/bio.734 |
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Luminescence |
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253 |
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