The roles of the two highly unstable components F and P involved in the bioluminescence of euphausiid shrimps
Abstract Bioluminescence of euphausiids takes place when a fluorescent tetrapyrrole F and a highly unstable protein P react in the presence of oxygen. A previous study on the euphausiid Meganyctiphanes norvegica indicated that F acts as a catalyst and P is consumed in the luminescence reaction, diff...
Published in: | Journal of Bioluminescence and Chemiluminescence |
---|---|
Main Author: | |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Wiley
1995
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1002/bio.1170100205 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fbio.1170100205 https://onlinelibrary.wiley.com/doi/pdf/10.1002/bio.1170100205 |
id |
crwiley:10.1002/bio.1170100205 |
---|---|
record_format |
openpolar |
spelling |
crwiley:10.1002/bio.1170100205 2024-06-23T07:54:32+00:00 The roles of the two highly unstable components F and P involved in the bioluminescence of euphausiid shrimps Shimomura, Osamu 1995 http://dx.doi.org/10.1002/bio.1170100205 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fbio.1170100205 https://onlinelibrary.wiley.com/doi/pdf/10.1002/bio.1170100205 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Bioluminescence and Chemiluminescence volume 10, issue 2, page 91-101 ISSN 0884-3996 1099-1271 journal-article 1995 crwiley https://doi.org/10.1002/bio.1170100205 2024-06-04T06:44:44Z Abstract Bioluminescence of euphausiids takes place when a fluorescent tetrapyrrole F and a highly unstable protein P react in the presence of oxygen. A previous study on the euphausiid Meganyctiphanes norvegica indicated that F acts as a catalyst and P is consumed in the luminescence reaction, differing from the luminescence system of dinoflagellates in which a tetrapyrrole luciferin, nearly identical to F, is enzymatically oxidized in the presence of dinoflagellate luciferase. In the present study, P was extracted from Euphausia pacifica as well as from M. norvegica , then purified separately by affinity chromatography on a column of biliverdin–Sepharose 4B, completing the whole process in less than 5h. The samples of P obtained from both species had a molecular weight of 600,000, a purity of about 80%, and a specific activity 50–100 times greater than that previously found. The activity of P rapidly decreased in solutions, even at 0°C, and the inactivation of P derived from M. norvegica was more than four times faster than that derived from E. pacifica . The kinetics of the luminescence reaction was investigated with F and P whose concentrations were systematically varied. The reaction was characteristically slow and involved two different reaction rates; the turnover number at 0°C was 30/h for the initial 20 min and 20/h after the initial 1 h. The total light emitted in a 50‐h period indicated that the bioluminescence quantum yield of F was about 0.6 at 0°C, and P recycled many times in the luminescence reaction. Thus, the present results conclusively show that F is a luciferin and P is a luciferase of an unusually slow‐working type, contrary to early report. Article in Journal/Newspaper Meganyctiphanes norvegica Wiley Online Library Journal of Bioluminescence and Chemiluminescence 10 2 91 101 |
institution |
Open Polar |
collection |
Wiley Online Library |
op_collection_id |
crwiley |
language |
English |
description |
Abstract Bioluminescence of euphausiids takes place when a fluorescent tetrapyrrole F and a highly unstable protein P react in the presence of oxygen. A previous study on the euphausiid Meganyctiphanes norvegica indicated that F acts as a catalyst and P is consumed in the luminescence reaction, differing from the luminescence system of dinoflagellates in which a tetrapyrrole luciferin, nearly identical to F, is enzymatically oxidized in the presence of dinoflagellate luciferase. In the present study, P was extracted from Euphausia pacifica as well as from M. norvegica , then purified separately by affinity chromatography on a column of biliverdin–Sepharose 4B, completing the whole process in less than 5h. The samples of P obtained from both species had a molecular weight of 600,000, a purity of about 80%, and a specific activity 50–100 times greater than that previously found. The activity of P rapidly decreased in solutions, even at 0°C, and the inactivation of P derived from M. norvegica was more than four times faster than that derived from E. pacifica . The kinetics of the luminescence reaction was investigated with F and P whose concentrations were systematically varied. The reaction was characteristically slow and involved two different reaction rates; the turnover number at 0°C was 30/h for the initial 20 min and 20/h after the initial 1 h. The total light emitted in a 50‐h period indicated that the bioluminescence quantum yield of F was about 0.6 at 0°C, and P recycled many times in the luminescence reaction. Thus, the present results conclusively show that F is a luciferin and P is a luciferase of an unusually slow‐working type, contrary to early report. |
format |
Article in Journal/Newspaper |
author |
Shimomura, Osamu |
spellingShingle |
Shimomura, Osamu The roles of the two highly unstable components F and P involved in the bioluminescence of euphausiid shrimps |
author_facet |
Shimomura, Osamu |
author_sort |
Shimomura, Osamu |
title |
The roles of the two highly unstable components F and P involved in the bioluminescence of euphausiid shrimps |
title_short |
The roles of the two highly unstable components F and P involved in the bioluminescence of euphausiid shrimps |
title_full |
The roles of the two highly unstable components F and P involved in the bioluminescence of euphausiid shrimps |
title_fullStr |
The roles of the two highly unstable components F and P involved in the bioluminescence of euphausiid shrimps |
title_full_unstemmed |
The roles of the two highly unstable components F and P involved in the bioluminescence of euphausiid shrimps |
title_sort |
roles of the two highly unstable components f and p involved in the bioluminescence of euphausiid shrimps |
publisher |
Wiley |
publishDate |
1995 |
url |
http://dx.doi.org/10.1002/bio.1170100205 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fbio.1170100205 https://onlinelibrary.wiley.com/doi/pdf/10.1002/bio.1170100205 |
genre |
Meganyctiphanes norvegica |
genre_facet |
Meganyctiphanes norvegica |
op_source |
Journal of Bioluminescence and Chemiluminescence volume 10, issue 2, page 91-101 ISSN 0884-3996 1099-1271 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/bio.1170100205 |
container_title |
Journal of Bioluminescence and Chemiluminescence |
container_volume |
10 |
container_issue |
2 |
container_start_page |
91 |
op_container_end_page |
101 |
_version_ |
1802646721957724160 |