Lateral Mobility of Pyrene in Model Membranes of Phospholipids with Different Chain Length
Abstract The coefficients of lateral diffusion, D p , of pyrene in lipid bilayers of several 1,2‐diacyl‐phosphatidyl cholines (distearyllecithin (DSL), di‐palmitoyllecithin (DPL), dimyristoyllecithin (DML) and dilauryllecithin (DLL) above the corresponding lipid phase transition were determined. D p...
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crwiley:10.1002/bbpc.19740780930 2024-06-02T08:05:49+00:00 Lateral Mobility of Pyrene in Model Membranes of Phospholipids with Different Chain Length Galla, H.‐J. Sackmann, E. 1974 http://dx.doi.org/10.1002/bbpc.19740780930 https://onlinelibrary.wiley.com/doi/pdf/10.1002/bbpc.19740780930 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Berichte der Bunsengesellschaft für physikalische Chemie volume 78, issue 9, page 949-953 ISSN 0005-9021 0005-9021 journal-article 1974 crwiley https://doi.org/10.1002/bbpc.19740780930 2024-05-06T07:02:12Z Abstract The coefficients of lateral diffusion, D p , of pyrene in lipid bilayers of several 1,2‐diacyl‐phosphatidyl cholines (distearyllecithin (DSL), di‐palmitoyllecithin (DPL), dimyristoyllecithin (DML) and dilauryllecithin (DLL) above the corresponding lipid phase transition were determined. D p was obtained from the second order rate constant, k a , of the pyrene excimer formation. It is found that the lateral mobility of an apolar molecule (such as pyrene) in the hydrophobic region of lipid lamella does not depend on the hydrocarbon chain length ( D p ≈︁ 2 · 10 −7 cm 2 /sec at 60°C). The pyren mobility is characterized by an activation energy of Δ E p + ≈︁ 10 kcal/mol. The experimental data are interpreted in terms of a simple model that is based on the assumption that the hopping frequency of a diffusing particle is determined by the rate of formation of free volume in the hydrocarbon moiety of the membrane. The latter is created by thermal activation of rotational isomers (essentially gtg‐kinks) in the hydrocarbon chains. The hopping frequency of the pyrene molecule is related to the jump frequency of the kinks along the hydrocarbon chains. Article in Journal/Newspaper DML Wiley Online Library Berichte der Bunsengesellschaft für physikalische Chemie 78 9 949 953 |
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Wiley Online Library |
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English |
description |
Abstract The coefficients of lateral diffusion, D p , of pyrene in lipid bilayers of several 1,2‐diacyl‐phosphatidyl cholines (distearyllecithin (DSL), di‐palmitoyllecithin (DPL), dimyristoyllecithin (DML) and dilauryllecithin (DLL) above the corresponding lipid phase transition were determined. D p was obtained from the second order rate constant, k a , of the pyrene excimer formation. It is found that the lateral mobility of an apolar molecule (such as pyrene) in the hydrophobic region of lipid lamella does not depend on the hydrocarbon chain length ( D p ≈︁ 2 · 10 −7 cm 2 /sec at 60°C). The pyren mobility is characterized by an activation energy of Δ E p + ≈︁ 10 kcal/mol. The experimental data are interpreted in terms of a simple model that is based on the assumption that the hopping frequency of a diffusing particle is determined by the rate of formation of free volume in the hydrocarbon moiety of the membrane. The latter is created by thermal activation of rotational isomers (essentially gtg‐kinks) in the hydrocarbon chains. The hopping frequency of the pyrene molecule is related to the jump frequency of the kinks along the hydrocarbon chains. |
format |
Article in Journal/Newspaper |
author |
Galla, H.‐J. Sackmann, E. |
spellingShingle |
Galla, H.‐J. Sackmann, E. Lateral Mobility of Pyrene in Model Membranes of Phospholipids with Different Chain Length |
author_facet |
Galla, H.‐J. Sackmann, E. |
author_sort |
Galla, H.‐J. |
title |
Lateral Mobility of Pyrene in Model Membranes of Phospholipids with Different Chain Length |
title_short |
Lateral Mobility of Pyrene in Model Membranes of Phospholipids with Different Chain Length |
title_full |
Lateral Mobility of Pyrene in Model Membranes of Phospholipids with Different Chain Length |
title_fullStr |
Lateral Mobility of Pyrene in Model Membranes of Phospholipids with Different Chain Length |
title_full_unstemmed |
Lateral Mobility of Pyrene in Model Membranes of Phospholipids with Different Chain Length |
title_sort |
lateral mobility of pyrene in model membranes of phospholipids with different chain length |
publisher |
Wiley |
publishDate |
1974 |
url |
http://dx.doi.org/10.1002/bbpc.19740780930 https://onlinelibrary.wiley.com/doi/pdf/10.1002/bbpc.19740780930 |
genre |
DML |
genre_facet |
DML |
op_source |
Berichte der Bunsengesellschaft für physikalische Chemie volume 78, issue 9, page 949-953 ISSN 0005-9021 0005-9021 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/bbpc.19740780930 |
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Berichte der Bunsengesellschaft für physikalische Chemie |
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78 |
container_issue |
9 |
container_start_page |
949 |
op_container_end_page |
953 |
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1800750694528974848 |