HYDROGEN PEROXIDE AND ECDYSONE IN THE CRYOPROTECTIVE DEHYDRATION STRATEGY OF Megaphorura Arctica (ONYCHIURIDAE: COLLEMBOLA)
The Arctic springtail, Megaphorura arctica, survives sub‐zero temperatures in a dehydrated state via trehalose‐dependent cryoprotective dehydration. Regulation of trehalose biosynthesis is complex; based in part on studies in yeast and fungi, its connection with oxidative stress caused by exposure o...
Published in: | Archives of Insect Biochemistry and Physiology |
---|---|
Main Authors: | , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Wiley
2012
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1002/arch.21073 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Farch.21073 https://onlinelibrary.wiley.com/doi/pdf/10.1002/arch.21073 |
id |
crwiley:10.1002/arch.21073 |
---|---|
record_format |
openpolar |
spelling |
crwiley:10.1002/arch.21073 2024-10-13T14:05:01+00:00 HYDROGEN PEROXIDE AND ECDYSONE IN THE CRYOPROTECTIVE DEHYDRATION STRATEGY OF Megaphorura Arctica (ONYCHIURIDAE: COLLEMBOLA) Grubor‐Lajšić, Gordana Petri, Edward T. Kojić, Danijela Purać, Jelena Popović, Željko D. Worland, Roger M. Clark, Melody S. Mojović, Miloš Blagojević, Duško P. 2012 http://dx.doi.org/10.1002/arch.21073 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Farch.21073 https://onlinelibrary.wiley.com/doi/pdf/10.1002/arch.21073 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Archives of Insect Biochemistry and Physiology volume 82, issue 2, page 59-70 ISSN 0739-4462 1520-6327 journal-article 2012 crwiley https://doi.org/10.1002/arch.21073 2024-09-17T04:47:35Z The Arctic springtail, Megaphorura arctica, survives sub‐zero temperatures in a dehydrated state via trehalose‐dependent cryoprotective dehydration. Regulation of trehalose biosynthesis is complex; based in part on studies in yeast and fungi, its connection with oxidative stress caused by exposure of cells to oxidants, such as hydrogen peroxide (H 2 O 2 ), or dehydration, is well documented. In this respect, we measured the amount of H 2 O 2 and antioxidant enzyme activities (superoxide dismutases: copper, zinc—CuZnSOD and manganese containing–MnSOD, and catalase—CAT), as the regulatory components determining H 2 O 2 concentrations, in Arctic springtails incubated at 5 °C (control) versus −2 °C (threshold temperature for trehalose biosynthesis). Because ecdysone also stimulates trehalose production in insects and regulates the expression of genes involved in redox homeostasis and antioxidant protection in Drosophila, we measured the levels of the active physiological form of ecdysone—20‐hydroxyecdysone (20‐HE). Significantly elevated H 2 O 2 and 20‐HE levels were observed in M. arctica incubated at −2 °C, supporting a link between ecdysone, H 2 O 2 , and trehalose levels during cryoprotective dehydration. CAT activity was found to be significantly lower in M. arctica incubated at −2 °C versus 5 °C, suggesting reduced H 2 O 2 breakdown. Furthermore, measurement of the free radical composition in Arctic springtails incubated at 5 °C (controls) versus −2 °C by Electron Paramagnetic Resonance spectroscopy revealed melanin‐derived free radicals at −2 °C, perhaps an additional source of H 2 O 2 . Our results suggest that H 2 O 2 and ecdysone play important roles in the cryoprotective dehydration process in M. arctica, linked with the regulation of trehalose biosynthesis. Article in Journal/Newspaper Arctic Springtail Wiley Online Library Arctic Archives of Insect Biochemistry and Physiology 82 2 59 70 |
institution |
Open Polar |
collection |
Wiley Online Library |
op_collection_id |
crwiley |
language |
English |
description |
The Arctic springtail, Megaphorura arctica, survives sub‐zero temperatures in a dehydrated state via trehalose‐dependent cryoprotective dehydration. Regulation of trehalose biosynthesis is complex; based in part on studies in yeast and fungi, its connection with oxidative stress caused by exposure of cells to oxidants, such as hydrogen peroxide (H 2 O 2 ), or dehydration, is well documented. In this respect, we measured the amount of H 2 O 2 and antioxidant enzyme activities (superoxide dismutases: copper, zinc—CuZnSOD and manganese containing–MnSOD, and catalase—CAT), as the regulatory components determining H 2 O 2 concentrations, in Arctic springtails incubated at 5 °C (control) versus −2 °C (threshold temperature for trehalose biosynthesis). Because ecdysone also stimulates trehalose production in insects and regulates the expression of genes involved in redox homeostasis and antioxidant protection in Drosophila, we measured the levels of the active physiological form of ecdysone—20‐hydroxyecdysone (20‐HE). Significantly elevated H 2 O 2 and 20‐HE levels were observed in M. arctica incubated at −2 °C, supporting a link between ecdysone, H 2 O 2 , and trehalose levels during cryoprotective dehydration. CAT activity was found to be significantly lower in M. arctica incubated at −2 °C versus 5 °C, suggesting reduced H 2 O 2 breakdown. Furthermore, measurement of the free radical composition in Arctic springtails incubated at 5 °C (controls) versus −2 °C by Electron Paramagnetic Resonance spectroscopy revealed melanin‐derived free radicals at −2 °C, perhaps an additional source of H 2 O 2 . Our results suggest that H 2 O 2 and ecdysone play important roles in the cryoprotective dehydration process in M. arctica, linked with the regulation of trehalose biosynthesis. |
format |
Article in Journal/Newspaper |
author |
Grubor‐Lajšić, Gordana Petri, Edward T. Kojić, Danijela Purać, Jelena Popović, Željko D. Worland, Roger M. Clark, Melody S. Mojović, Miloš Blagojević, Duško P. |
spellingShingle |
Grubor‐Lajšić, Gordana Petri, Edward T. Kojić, Danijela Purać, Jelena Popović, Željko D. Worland, Roger M. Clark, Melody S. Mojović, Miloš Blagojević, Duško P. HYDROGEN PEROXIDE AND ECDYSONE IN THE CRYOPROTECTIVE DEHYDRATION STRATEGY OF Megaphorura Arctica (ONYCHIURIDAE: COLLEMBOLA) |
author_facet |
Grubor‐Lajšić, Gordana Petri, Edward T. Kojić, Danijela Purać, Jelena Popović, Željko D. Worland, Roger M. Clark, Melody S. Mojović, Miloš Blagojević, Duško P. |
author_sort |
Grubor‐Lajšić, Gordana |
title |
HYDROGEN PEROXIDE AND ECDYSONE IN THE CRYOPROTECTIVE DEHYDRATION STRATEGY OF Megaphorura Arctica (ONYCHIURIDAE: COLLEMBOLA) |
title_short |
HYDROGEN PEROXIDE AND ECDYSONE IN THE CRYOPROTECTIVE DEHYDRATION STRATEGY OF Megaphorura Arctica (ONYCHIURIDAE: COLLEMBOLA) |
title_full |
HYDROGEN PEROXIDE AND ECDYSONE IN THE CRYOPROTECTIVE DEHYDRATION STRATEGY OF Megaphorura Arctica (ONYCHIURIDAE: COLLEMBOLA) |
title_fullStr |
HYDROGEN PEROXIDE AND ECDYSONE IN THE CRYOPROTECTIVE DEHYDRATION STRATEGY OF Megaphorura Arctica (ONYCHIURIDAE: COLLEMBOLA) |
title_full_unstemmed |
HYDROGEN PEROXIDE AND ECDYSONE IN THE CRYOPROTECTIVE DEHYDRATION STRATEGY OF Megaphorura Arctica (ONYCHIURIDAE: COLLEMBOLA) |
title_sort |
hydrogen peroxide and ecdysone in the cryoprotective dehydration strategy of megaphorura arctica (onychiuridae: collembola) |
publisher |
Wiley |
publishDate |
2012 |
url |
http://dx.doi.org/10.1002/arch.21073 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Farch.21073 https://onlinelibrary.wiley.com/doi/pdf/10.1002/arch.21073 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Springtail |
genre_facet |
Arctic Springtail |
op_source |
Archives of Insect Biochemistry and Physiology volume 82, issue 2, page 59-70 ISSN 0739-4462 1520-6327 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/arch.21073 |
container_title |
Archives of Insect Biochemistry and Physiology |
container_volume |
82 |
container_issue |
2 |
container_start_page |
59 |
op_container_end_page |
70 |
_version_ |
1812810869566865408 |