Liver of juvenile atlantic salmon, Salmo salar L.: A light, transmission, and scanning electron microscopic study, with special reference to the sinusoid
Abstract Background : This report provides a detailed description of sinusoidal and perisinusoidal structures in the normal liver of the juvenile Atlantic salmon ( Salmo salar L.), a teleost species. Methods : The liver was studied by light, transmission, and scanning electron microscopy, and organ...
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crwiley:10.1002/ar.1092400302 2024-06-02T08:03:20+00:00 Liver of juvenile atlantic salmon, Salmo salar L.: A light, transmission, and scanning electron microscopic study, with special reference to the sinusoid Speilberg, Lars Evensen, Øystein Nafstad, Per 1994 http://dx.doi.org/10.1002/ar.1092400302 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Far.1092400302 https://onlinelibrary.wiley.com/doi/pdf/10.1002/ar.1092400302 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor The Anatomical Record volume 240, issue 3, page 291-307 ISSN 0003-276X 1097-0185 journal-article 1994 crwiley https://doi.org/10.1002/ar.1092400302 2024-05-03T11:34:05Z Abstract Background : This report provides a detailed description of sinusoidal and perisinusoidal structures in the normal liver of the juvenile Atlantic salmon ( Salmo salar L.), a teleost species. Methods : The liver was studied by light, transmission, and scanning electron microscopy, and organ specimens were sampled after retrograde, whole‐body perfusion through the dorsal aorta using 3% glutaraldehyde. Detailed characterization of perisinusoidal stellate cells also included immunohistochemical staining for desmin and evaluation of autofluorescence of the same cells upon excitation in ultraviolet (UV) light. Results : The sinusoid is lined by one cell type only: the endothelial cell. No intraluminal pit cells or Kupffer cells are present. The space of Disse contains reticulin fibres, visualized by Gomori's silver stain, and perisinusoidal stellate cells (PSC). PSC exhibited autofluorescence in UV light, indicating that these cells store vitamin A in cytoplasmic lipid droplets. Immunohistochemically, PSC were found negative for desmin. The space of Disse, extending deep down between adjacent hepatocytes, receives long, slender microvilli from parenchymal cells. In addition to scattered macrophages, interhepatocytic cells (IHC) are found perisinusoidally. Hepatocytes of Atlantic salmon form branching and anastomosing tubules. Conclusions : The sinusoids of Atlantic salmon liver are lined by a fenestrated endothelium, with PSC located in the space of Disse, with macrophages and IHC as inhabitants of the interhepatocytic space. IHC show ultrastructural similarities to mammalian pit cells and teleostean large granular lymphocytes, as well as to piscine monocytes. PSC might be storage cells for vitamin A in Atlantic salmon as shown by autofluorescence in these cells, while immunohistochemical studies indicate that desmin does not seem to be an adequate immunohistochemical marker for PSC in the juvenile Atlantic salmon. Methodologically, fixation for electron microscopy was performed by a new and convenient ... Article in Journal/Newspaper Atlantic salmon Salmo salar Wiley Online Library The Anatomical Record 240 3 291 307 |
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Open Polar |
collection |
Wiley Online Library |
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crwiley |
language |
English |
description |
Abstract Background : This report provides a detailed description of sinusoidal and perisinusoidal structures in the normal liver of the juvenile Atlantic salmon ( Salmo salar L.), a teleost species. Methods : The liver was studied by light, transmission, and scanning electron microscopy, and organ specimens were sampled after retrograde, whole‐body perfusion through the dorsal aorta using 3% glutaraldehyde. Detailed characterization of perisinusoidal stellate cells also included immunohistochemical staining for desmin and evaluation of autofluorescence of the same cells upon excitation in ultraviolet (UV) light. Results : The sinusoid is lined by one cell type only: the endothelial cell. No intraluminal pit cells or Kupffer cells are present. The space of Disse contains reticulin fibres, visualized by Gomori's silver stain, and perisinusoidal stellate cells (PSC). PSC exhibited autofluorescence in UV light, indicating that these cells store vitamin A in cytoplasmic lipid droplets. Immunohistochemically, PSC were found negative for desmin. The space of Disse, extending deep down between adjacent hepatocytes, receives long, slender microvilli from parenchymal cells. In addition to scattered macrophages, interhepatocytic cells (IHC) are found perisinusoidally. Hepatocytes of Atlantic salmon form branching and anastomosing tubules. Conclusions : The sinusoids of Atlantic salmon liver are lined by a fenestrated endothelium, with PSC located in the space of Disse, with macrophages and IHC as inhabitants of the interhepatocytic space. IHC show ultrastructural similarities to mammalian pit cells and teleostean large granular lymphocytes, as well as to piscine monocytes. PSC might be storage cells for vitamin A in Atlantic salmon as shown by autofluorescence in these cells, while immunohistochemical studies indicate that desmin does not seem to be an adequate immunohistochemical marker for PSC in the juvenile Atlantic salmon. Methodologically, fixation for electron microscopy was performed by a new and convenient ... |
format |
Article in Journal/Newspaper |
author |
Speilberg, Lars Evensen, Øystein Nafstad, Per |
spellingShingle |
Speilberg, Lars Evensen, Øystein Nafstad, Per Liver of juvenile atlantic salmon, Salmo salar L.: A light, transmission, and scanning electron microscopic study, with special reference to the sinusoid |
author_facet |
Speilberg, Lars Evensen, Øystein Nafstad, Per |
author_sort |
Speilberg, Lars |
title |
Liver of juvenile atlantic salmon, Salmo salar L.: A light, transmission, and scanning electron microscopic study, with special reference to the sinusoid |
title_short |
Liver of juvenile atlantic salmon, Salmo salar L.: A light, transmission, and scanning electron microscopic study, with special reference to the sinusoid |
title_full |
Liver of juvenile atlantic salmon, Salmo salar L.: A light, transmission, and scanning electron microscopic study, with special reference to the sinusoid |
title_fullStr |
Liver of juvenile atlantic salmon, Salmo salar L.: A light, transmission, and scanning electron microscopic study, with special reference to the sinusoid |
title_full_unstemmed |
Liver of juvenile atlantic salmon, Salmo salar L.: A light, transmission, and scanning electron microscopic study, with special reference to the sinusoid |
title_sort |
liver of juvenile atlantic salmon, salmo salar l.: a light, transmission, and scanning electron microscopic study, with special reference to the sinusoid |
publisher |
Wiley |
publishDate |
1994 |
url |
http://dx.doi.org/10.1002/ar.1092400302 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Far.1092400302 https://onlinelibrary.wiley.com/doi/pdf/10.1002/ar.1092400302 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
The Anatomical Record volume 240, issue 3, page 291-307 ISSN 0003-276X 1097-0185 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/ar.1092400302 |
container_title |
The Anatomical Record |
container_volume |
240 |
container_issue |
3 |
container_start_page |
291 |
op_container_end_page |
307 |
_version_ |
1800747846785302528 |