Ultrastructural cytochemistry of aortic microfibrils in the arctic lamprey, Lampetra japonica
Abstract In the ventral aorta of lamprey, microfibrils are major components of the extracellilar matrix. With special refeence to these microfibrils, we have cytochemically exmined the ventral aorta, utilizaing the tannic acid (pH 7.0)‐uranyl acetate (TA‐UA) method, elastase en bloc digestion, Thiér...
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crwiley:10.1002/ar.1092230207 2024-06-02T08:02:13+00:00 Ultrastructural cytochemistry of aortic microfibrils in the arctic lamprey, Lampetra japonica Isokawa, Keitaro Takagi, Minoru Toda, Yoshihisa 1989 http://dx.doi.org/10.1002/ar.1092230207 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Far.1092230207 https://onlinelibrary.wiley.com/doi/pdf/10.1002/ar.1092230207 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor The Anatomical Record volume 223, issue 2, page 158-164 ISSN 0003-276X 1097-0185 journal-article 1989 crwiley https://doi.org/10.1002/ar.1092230207 2024-05-03T11:52:18Z Abstract In the ventral aorta of lamprey, microfibrils are major components of the extracellilar matrix. With special refeence to these microfibrils, we have cytochemically exmined the ventral aorta, utilizaing the tannic acid (pH 7.0)‐uranyl acetate (TA‐UA) method, elastase en bloc digestion, Thiéry's periodis acid‐thiocarbohydrazide‐silver proteinate (PA‐TCH‐SP) method, and ferritin‐ or horseradish peroxidase‐labeledd concanacalin A (Con A) methods. The lamprey microfirils were strongly stained with PA‐TCH‐SP and both Con A methods, but did not show TA‐UA staining nor elastase sensitivity. These cytochemical properties of lamprey microfibrils are identical with those of mammalian elastin‐associated microfibrils. On the other hand, in spite of extensive examination, TA‐UA positive and elastase‐sensitive extracellular components were not found, so that lamprey ventral aorta does not appear to contain elasin. These results indicate that lamprey lamprey aortic connective tissue contains microfibrils as elastic components, but deposition of amorphous elastin dose not occur. Article in Journal/Newspaper Arctic Wiley Online Library Arctic The Anatomical Record 223 2 158 164 |
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Wiley Online Library |
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English |
description |
Abstract In the ventral aorta of lamprey, microfibrils are major components of the extracellilar matrix. With special refeence to these microfibrils, we have cytochemically exmined the ventral aorta, utilizaing the tannic acid (pH 7.0)‐uranyl acetate (TA‐UA) method, elastase en bloc digestion, Thiéry's periodis acid‐thiocarbohydrazide‐silver proteinate (PA‐TCH‐SP) method, and ferritin‐ or horseradish peroxidase‐labeledd concanacalin A (Con A) methods. The lamprey microfirils were strongly stained with PA‐TCH‐SP and both Con A methods, but did not show TA‐UA staining nor elastase sensitivity. These cytochemical properties of lamprey microfibrils are identical with those of mammalian elastin‐associated microfibrils. On the other hand, in spite of extensive examination, TA‐UA positive and elastase‐sensitive extracellular components were not found, so that lamprey ventral aorta does not appear to contain elasin. These results indicate that lamprey lamprey aortic connective tissue contains microfibrils as elastic components, but deposition of amorphous elastin dose not occur. |
format |
Article in Journal/Newspaper |
author |
Isokawa, Keitaro Takagi, Minoru Toda, Yoshihisa |
spellingShingle |
Isokawa, Keitaro Takagi, Minoru Toda, Yoshihisa Ultrastructural cytochemistry of aortic microfibrils in the arctic lamprey, Lampetra japonica |
author_facet |
Isokawa, Keitaro Takagi, Minoru Toda, Yoshihisa |
author_sort |
Isokawa, Keitaro |
title |
Ultrastructural cytochemistry of aortic microfibrils in the arctic lamprey, Lampetra japonica |
title_short |
Ultrastructural cytochemistry of aortic microfibrils in the arctic lamprey, Lampetra japonica |
title_full |
Ultrastructural cytochemistry of aortic microfibrils in the arctic lamprey, Lampetra japonica |
title_fullStr |
Ultrastructural cytochemistry of aortic microfibrils in the arctic lamprey, Lampetra japonica |
title_full_unstemmed |
Ultrastructural cytochemistry of aortic microfibrils in the arctic lamprey, Lampetra japonica |
title_sort |
ultrastructural cytochemistry of aortic microfibrils in the arctic lamprey, lampetra japonica |
publisher |
Wiley |
publishDate |
1989 |
url |
http://dx.doi.org/10.1002/ar.1092230207 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Far.1092230207 https://onlinelibrary.wiley.com/doi/pdf/10.1002/ar.1092230207 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
The Anatomical Record volume 223, issue 2, page 158-164 ISSN 0003-276X 1097-0185 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/ar.1092230207 |
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The Anatomical Record |
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223 |
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2 |
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158 |
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164 |
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1800746710539960320 |