A quantitative PCR‐based environmental DNA assay for detecting Atlantic salmon ( Salmo salar L.)
Abstract The Atlantic salmon ( Salmo salar L.) has worldwide ecological, cultural, and economic importance. The species has undergone extensive decline across its native range, yet concerns have been raised about its invasive potential in the Pacific. Knowledge on the distribution of this species is...
Published in: | Aquatic Conservation: Marine and Freshwater Ecosystems |
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crwiley:10.1002/aqc.2931 2024-05-19T07:37:31+00:00 A quantitative PCR‐based environmental DNA assay for detecting Atlantic salmon ( Salmo salar L.) Atkinson, Siobhán Carlsson, Jeanette E.L. Ball, Bernard Egan, Damian Kelly‐Quinn, Mary Whelan, Ken Carlsson, Jens Atlantic Salmon Trust and Irish Environmental Protection Agency 2018 http://dx.doi.org/10.1002/aqc.2931 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Faqc.2931 https://onlinelibrary.wiley.com/doi/pdf/10.1002/aqc.2931 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Aquatic Conservation: Marine and Freshwater Ecosystems volume 28, issue 5, page 1238-1243 ISSN 1052-7613 1099-0755 journal-article 2018 crwiley https://doi.org/10.1002/aqc.2931 2024-04-25T08:27:34Z Abstract The Atlantic salmon ( Salmo salar L.) has worldwide ecological, cultural, and economic importance. The species has undergone extensive decline across its native range, yet concerns have been raised about its invasive potential in the Pacific. Knowledge on the distribution of this species is vital for addressing conservation goals. This study presents an environmental DNA assay to detect S. salar in water samples, using quantitative polymerase chain reaction technology. Species‐specific primers and a minor groove binding probe were designed for the assay, based on the mitochondrial cytochrome oxidase I gene. The results of this study indicate that environmental DNA is a highly effective tool for detecting S. salar in situ, and could provide an alternative, non‐invasive method for determining the distribution of this species. Article in Journal/Newspaper Atlantic salmon Salmo salar Wiley Online Library Aquatic Conservation: Marine and Freshwater Ecosystems 28 5 1238 1243 |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Abstract The Atlantic salmon ( Salmo salar L.) has worldwide ecological, cultural, and economic importance. The species has undergone extensive decline across its native range, yet concerns have been raised about its invasive potential in the Pacific. Knowledge on the distribution of this species is vital for addressing conservation goals. This study presents an environmental DNA assay to detect S. salar in water samples, using quantitative polymerase chain reaction technology. Species‐specific primers and a minor groove binding probe were designed for the assay, based on the mitochondrial cytochrome oxidase I gene. The results of this study indicate that environmental DNA is a highly effective tool for detecting S. salar in situ, and could provide an alternative, non‐invasive method for determining the distribution of this species. |
author2 |
Atlantic Salmon Trust and Irish Environmental Protection Agency |
format |
Article in Journal/Newspaper |
author |
Atkinson, Siobhán Carlsson, Jeanette E.L. Ball, Bernard Egan, Damian Kelly‐Quinn, Mary Whelan, Ken Carlsson, Jens |
spellingShingle |
Atkinson, Siobhán Carlsson, Jeanette E.L. Ball, Bernard Egan, Damian Kelly‐Quinn, Mary Whelan, Ken Carlsson, Jens A quantitative PCR‐based environmental DNA assay for detecting Atlantic salmon ( Salmo salar L.) |
author_facet |
Atkinson, Siobhán Carlsson, Jeanette E.L. Ball, Bernard Egan, Damian Kelly‐Quinn, Mary Whelan, Ken Carlsson, Jens |
author_sort |
Atkinson, Siobhán |
title |
A quantitative PCR‐based environmental DNA assay for detecting Atlantic salmon ( Salmo salar L.) |
title_short |
A quantitative PCR‐based environmental DNA assay for detecting Atlantic salmon ( Salmo salar L.) |
title_full |
A quantitative PCR‐based environmental DNA assay for detecting Atlantic salmon ( Salmo salar L.) |
title_fullStr |
A quantitative PCR‐based environmental DNA assay for detecting Atlantic salmon ( Salmo salar L.) |
title_full_unstemmed |
A quantitative PCR‐based environmental DNA assay for detecting Atlantic salmon ( Salmo salar L.) |
title_sort |
quantitative pcr‐based environmental dna assay for detecting atlantic salmon ( salmo salar l.) |
publisher |
Wiley |
publishDate |
2018 |
url |
http://dx.doi.org/10.1002/aqc.2931 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Faqc.2931 https://onlinelibrary.wiley.com/doi/pdf/10.1002/aqc.2931 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Aquatic Conservation: Marine and Freshwater Ecosystems volume 28, issue 5, page 1238-1243 ISSN 1052-7613 1099-0755 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/aqc.2931 |
container_title |
Aquatic Conservation: Marine and Freshwater Ecosystems |
container_volume |
28 |
container_issue |
5 |
container_start_page |
1238 |
op_container_end_page |
1243 |
_version_ |
1799476836896342016 |