Prediction and comparison of textural properties of magnetic copolymer supports for enzyme immobilization
Abstract Magnetic polymers supports have proven to be valuable materials for enzyme immobilization, as they allow recovering the catalyst by magnetic separation, precluding the need for costly and time‐consuming separation steps. In this study, magnetic copolymer supports were synthesized using styr...
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crwiley:10.1002/app.49258 2024-06-02T07:58:14+00:00 Prediction and comparison of textural properties of magnetic copolymer supports for enzyme immobilization Rosa, Cintia Maria Rodrigues da Silva, Mateus Vinícius Casagrande de Aguiar, Leandro Gonçalves de Castro, Heizir Ferreira Freitas, Larissa Coordenação de Aperfeiçoamento de Pessoal de Nível Superior Fundação de Amparo à Pesquisa do Estado de São Paulo 2020 http://dx.doi.org/10.1002/app.49258 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fapp.49258 https://onlinelibrary.wiley.com/doi/pdf/10.1002/app.49258 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/app.49258 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Applied Polymer Science volume 137, issue 41 ISSN 0021-8995 1097-4628 journal-article 2020 crwiley https://doi.org/10.1002/app.49258 2024-05-03T12:03:10Z Abstract Magnetic polymers supports have proven to be valuable materials for enzyme immobilization, as they allow recovering the catalyst by magnetic separation, precluding the need for costly and time‐consuming separation steps. In this study, magnetic copolymer supports were synthesized using styrene (STY) and different crosslinking agents (divinylbenzene, ethylene glycol dimethacrylate, or triethylene glycol dimethacrylate) and initiators (azobisisobutyronitrile or benzoyl peroxide) and used to immobilize Candida antarctica lipase B (CALB). The aim was to obtain biocatalysts with high enzymatic activity and satisfactory morphological properties for use in biotransformation reactions. Two morphological properties known to influence the immobilization yield were taken into consideration, specific surface area, and swelling index. Experimental data were compared to the predictions of a model based on molar balance, method of moments, numerical fractionation, and elementary gel structures. The high correlation ( R 2 = 0.9974) between experimental and predicted values demonstrated the suitability of the model for estimating the textural properties of enzyme supports. CALB was successfully immobilized, showing high hydrolytic activity (500–700 U g −1 ) and good thermal stability at 50°C. CALB/STY‐EGDMA‐M was 14 times more stable than free CALB. The results confirm the efficiency of the immobilization method and the suitability of the copolymers for enzyme immobilization. Article in Journal/Newspaper Antarc* Antarctica Wiley Online Library Journal of Applied Polymer Science 137 41 |
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English |
description |
Abstract Magnetic polymers supports have proven to be valuable materials for enzyme immobilization, as they allow recovering the catalyst by magnetic separation, precluding the need for costly and time‐consuming separation steps. In this study, magnetic copolymer supports were synthesized using styrene (STY) and different crosslinking agents (divinylbenzene, ethylene glycol dimethacrylate, or triethylene glycol dimethacrylate) and initiators (azobisisobutyronitrile or benzoyl peroxide) and used to immobilize Candida antarctica lipase B (CALB). The aim was to obtain biocatalysts with high enzymatic activity and satisfactory morphological properties for use in biotransformation reactions. Two morphological properties known to influence the immobilization yield were taken into consideration, specific surface area, and swelling index. Experimental data were compared to the predictions of a model based on molar balance, method of moments, numerical fractionation, and elementary gel structures. The high correlation ( R 2 = 0.9974) between experimental and predicted values demonstrated the suitability of the model for estimating the textural properties of enzyme supports. CALB was successfully immobilized, showing high hydrolytic activity (500–700 U g −1 ) and good thermal stability at 50°C. CALB/STY‐EGDMA‐M was 14 times more stable than free CALB. The results confirm the efficiency of the immobilization method and the suitability of the copolymers for enzyme immobilization. |
author2 |
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior Fundação de Amparo à Pesquisa do Estado de São Paulo |
format |
Article in Journal/Newspaper |
author |
Rosa, Cintia Maria Rodrigues da Silva, Mateus Vinícius Casagrande de Aguiar, Leandro Gonçalves de Castro, Heizir Ferreira Freitas, Larissa |
spellingShingle |
Rosa, Cintia Maria Rodrigues da Silva, Mateus Vinícius Casagrande de Aguiar, Leandro Gonçalves de Castro, Heizir Ferreira Freitas, Larissa Prediction and comparison of textural properties of magnetic copolymer supports for enzyme immobilization |
author_facet |
Rosa, Cintia Maria Rodrigues da Silva, Mateus Vinícius Casagrande de Aguiar, Leandro Gonçalves de Castro, Heizir Ferreira Freitas, Larissa |
author_sort |
Rosa, Cintia Maria Rodrigues |
title |
Prediction and comparison of textural properties of magnetic copolymer supports for enzyme immobilization |
title_short |
Prediction and comparison of textural properties of magnetic copolymer supports for enzyme immobilization |
title_full |
Prediction and comparison of textural properties of magnetic copolymer supports for enzyme immobilization |
title_fullStr |
Prediction and comparison of textural properties of magnetic copolymer supports for enzyme immobilization |
title_full_unstemmed |
Prediction and comparison of textural properties of magnetic copolymer supports for enzyme immobilization |
title_sort |
prediction and comparison of textural properties of magnetic copolymer supports for enzyme immobilization |
publisher |
Wiley |
publishDate |
2020 |
url |
http://dx.doi.org/10.1002/app.49258 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fapp.49258 https://onlinelibrary.wiley.com/doi/pdf/10.1002/app.49258 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/app.49258 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Journal of Applied Polymer Science volume 137, issue 41 ISSN 0021-8995 1097-4628 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/app.49258 |
container_title |
Journal of Applied Polymer Science |
container_volume |
137 |
container_issue |
41 |
_version_ |
1800741520183132160 |