Pilot‐scale development of core–shell polymer supports for the immobilization of recombinant lipase B from Candida antarctica and their application in the production of ethyl esters from residual fatty acids
ABSTRACT A recombinant lipase B from Candida antarctica (LipB) in Pichia pastoris was synthesized through submerged fermentation using crude glycerin as substrate. The immobilization of this enzyme on the core–shell polymeric supports is an effective alternative for its application. The supports wit...
Published in: | Journal of Applied Polymer Science |
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Online Access: | http://dx.doi.org/10.1002/app.46727 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fapp.46727 https://onlinelibrary.wiley.com/doi/pdf/10.1002/app.46727 |
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crwiley:10.1002/app.46727 2024-06-02T07:58:21+00:00 Pilot‐scale development of core–shell polymer supports for the immobilization of recombinant lipase B from Candida antarctica and their application in the production of ethyl esters from residual fatty acids Cipolatti, Eliane Pereira Pinto, Martina Costa Cerqueira Robert, Julia de Macedo da Silva, Tabita Pizoni Beralto, Thamires da Costa Santos, Jorge G. F. de Castro, Rui de Paula Vieira Fernandez‐Lafuente, Roberto Manoel, Evelin Andrade Pinto, José Carlos Freire, Denise Maria Guimarães CNPq CAPES FAPERJ MINECO 2018 http://dx.doi.org/10.1002/app.46727 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fapp.46727 https://onlinelibrary.wiley.com/doi/pdf/10.1002/app.46727 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Applied Polymer Science volume 135, issue 40 ISSN 0021-8995 1097-4628 journal-article 2018 crwiley https://doi.org/10.1002/app.46727 2024-05-03T11:53:26Z ABSTRACT A recombinant lipase B from Candida antarctica (LipB) in Pichia pastoris was synthesized through submerged fermentation using crude glycerin as substrate. The immobilization of this enzyme on the core–shell polymeric supports is an effective alternative for its application. The supports with distinct levels of hydrophobicity were produced through combined suspension and emulsion polymerization in pilot scale. Particles with distinct compositions were synthesized (PMMA/PMMA; PMMA ‐co‐ DVB/PMMA ‐co‐ DVB; and PS ‐co‐ DVB/PS ‐co‐ DVB) and employed on the immobilization of the produced lipase (LipB) and the commercial enzyme (CalB). The morphological properties (specific area, average pore diameter, specific volume of pores, and hydrophobicity level) and the influence of the polymerization conditions on the morphology of the supports were studied. The thermal stability of such biocatalysts was also investigated in the presence of calcium cation (Ca +2 ), maintained 100% of the activity after 3 h at 50°C when the PMMA ‐co‐ DVB/PMMA ‐co‐ DVB was employed. The synthesized enzyme and supports manufactured in pilot scale were employed successfully for production of esters using residual fatty acids as substrates, adding value to these raw materials and increasing the ranges of possible applications. Article in Journal/Newspaper Antarc* Antarctica Wiley Online Library Journal of Applied Polymer Science 135 40 46727 |
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Wiley Online Library |
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crwiley |
language |
English |
description |
ABSTRACT A recombinant lipase B from Candida antarctica (LipB) in Pichia pastoris was synthesized through submerged fermentation using crude glycerin as substrate. The immobilization of this enzyme on the core–shell polymeric supports is an effective alternative for its application. The supports with distinct levels of hydrophobicity were produced through combined suspension and emulsion polymerization in pilot scale. Particles with distinct compositions were synthesized (PMMA/PMMA; PMMA ‐co‐ DVB/PMMA ‐co‐ DVB; and PS ‐co‐ DVB/PS ‐co‐ DVB) and employed on the immobilization of the produced lipase (LipB) and the commercial enzyme (CalB). The morphological properties (specific area, average pore diameter, specific volume of pores, and hydrophobicity level) and the influence of the polymerization conditions on the morphology of the supports were studied. The thermal stability of such biocatalysts was also investigated in the presence of calcium cation (Ca +2 ), maintained 100% of the activity after 3 h at 50°C when the PMMA ‐co‐ DVB/PMMA ‐co‐ DVB was employed. The synthesized enzyme and supports manufactured in pilot scale were employed successfully for production of esters using residual fatty acids as substrates, adding value to these raw materials and increasing the ranges of possible applications. |
author2 |
CNPq CAPES FAPERJ MINECO |
format |
Article in Journal/Newspaper |
author |
Cipolatti, Eliane Pereira Pinto, Martina Costa Cerqueira Robert, Julia de Macedo da Silva, Tabita Pizoni Beralto, Thamires da Costa Santos, Jorge G. F. de Castro, Rui de Paula Vieira Fernandez‐Lafuente, Roberto Manoel, Evelin Andrade Pinto, José Carlos Freire, Denise Maria Guimarães |
spellingShingle |
Cipolatti, Eliane Pereira Pinto, Martina Costa Cerqueira Robert, Julia de Macedo da Silva, Tabita Pizoni Beralto, Thamires da Costa Santos, Jorge G. F. de Castro, Rui de Paula Vieira Fernandez‐Lafuente, Roberto Manoel, Evelin Andrade Pinto, José Carlos Freire, Denise Maria Guimarães Pilot‐scale development of core–shell polymer supports for the immobilization of recombinant lipase B from Candida antarctica and their application in the production of ethyl esters from residual fatty acids |
author_facet |
Cipolatti, Eliane Pereira Pinto, Martina Costa Cerqueira Robert, Julia de Macedo da Silva, Tabita Pizoni Beralto, Thamires da Costa Santos, Jorge G. F. de Castro, Rui de Paula Vieira Fernandez‐Lafuente, Roberto Manoel, Evelin Andrade Pinto, José Carlos Freire, Denise Maria Guimarães |
author_sort |
Cipolatti, Eliane Pereira |
title |
Pilot‐scale development of core–shell polymer supports for the immobilization of recombinant lipase B from Candida antarctica and their application in the production of ethyl esters from residual fatty acids |
title_short |
Pilot‐scale development of core–shell polymer supports for the immobilization of recombinant lipase B from Candida antarctica and their application in the production of ethyl esters from residual fatty acids |
title_full |
Pilot‐scale development of core–shell polymer supports for the immobilization of recombinant lipase B from Candida antarctica and their application in the production of ethyl esters from residual fatty acids |
title_fullStr |
Pilot‐scale development of core–shell polymer supports for the immobilization of recombinant lipase B from Candida antarctica and their application in the production of ethyl esters from residual fatty acids |
title_full_unstemmed |
Pilot‐scale development of core–shell polymer supports for the immobilization of recombinant lipase B from Candida antarctica and their application in the production of ethyl esters from residual fatty acids |
title_sort |
pilot‐scale development of core–shell polymer supports for the immobilization of recombinant lipase b from candida antarctica and their application in the production of ethyl esters from residual fatty acids |
publisher |
Wiley |
publishDate |
2018 |
url |
http://dx.doi.org/10.1002/app.46727 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fapp.46727 https://onlinelibrary.wiley.com/doi/pdf/10.1002/app.46727 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Journal of Applied Polymer Science volume 135, issue 40 ISSN 0021-8995 1097-4628 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/app.46727 |
container_title |
Journal of Applied Polymer Science |
container_volume |
135 |
container_issue |
40 |
container_start_page |
46727 |
_version_ |
1800741674287104000 |