Methylation of arsenic by anaerobic microbial consortia isolated from lake sediment
Abstract Anaerobic enrichment cultures, isolated from arsenic–contaminated lake sediment in the Canadian sub–arctic and grown in five selective media, methylated arsenate/arsenite to produce mono−, di− and tri–methyl arsenicals. The extent of methylation and methylarsenic species produced varied wit...
Published in: | Applied Organometallic Chemistry |
---|---|
Main Authors: | , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Wiley
1994
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1002/aoc.590080416 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Faoc.590080416 https://onlinelibrary.wiley.com/doi/pdf/10.1002/aoc.590080416 |
id |
crwiley:10.1002/aoc.590080416 |
---|---|
record_format |
openpolar |
spelling |
crwiley:10.1002/aoc.590080416 2024-09-09T19:24:56+00:00 Methylation of arsenic by anaerobic microbial consortia isolated from lake sediment Bright, D. A. Brock, S. Reimer, K. J. Cullen, W. R. Hewitt, G. M. Jafaar, J. 1994 http://dx.doi.org/10.1002/aoc.590080416 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Faoc.590080416 https://onlinelibrary.wiley.com/doi/pdf/10.1002/aoc.590080416 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Applied Organometallic Chemistry volume 8, issue 4, page 415-422 ISSN 0268-2605 1099-0739 journal-article 1994 crwiley https://doi.org/10.1002/aoc.590080416 2024-06-20T04:23:07Z Abstract Anaerobic enrichment cultures, isolated from arsenic–contaminated lake sediment in the Canadian sub–arctic and grown in five selective media, methylated arsenate/arsenite to produce mono−, di− and tri–methyl arsenicals. The extent of methylation and methylarsenic species produced varied with the type of enrichment. Iron–reducing, manganese–reducing, sulfate–reducing and broad–spectrum anaerobic heterotrophic mixed cultures all produced methylarsenicals. Sulfate–reducing cultures produced higher concentrations of methylarsenicals (especially trimethyl species) than iron‐ or maganese–reducers. There is evidence that several of the methylarsenicals, which were hydride–reactive at pH 6, were methylarsenic(III) thiols. The organoarsenicals produced by enrichment cultures were the same as those detected in the porewater of the lake sediments used to initiate the enrichment cultures. Overall, this study demonstrates that microbes from anaerobic lake sediments can methylate (and demethylate) arsenic, a capability shared by manganese−, iron−, and sulfate–reducing microbial consortia. Article in Journal/Newspaper Arctic Wiley Online Library Arctic Applied Organometallic Chemistry 8 4 415 422 |
institution |
Open Polar |
collection |
Wiley Online Library |
op_collection_id |
crwiley |
language |
English |
description |
Abstract Anaerobic enrichment cultures, isolated from arsenic–contaminated lake sediment in the Canadian sub–arctic and grown in five selective media, methylated arsenate/arsenite to produce mono−, di− and tri–methyl arsenicals. The extent of methylation and methylarsenic species produced varied with the type of enrichment. Iron–reducing, manganese–reducing, sulfate–reducing and broad–spectrum anaerobic heterotrophic mixed cultures all produced methylarsenicals. Sulfate–reducing cultures produced higher concentrations of methylarsenicals (especially trimethyl species) than iron‐ or maganese–reducers. There is evidence that several of the methylarsenicals, which were hydride–reactive at pH 6, were methylarsenic(III) thiols. The organoarsenicals produced by enrichment cultures were the same as those detected in the porewater of the lake sediments used to initiate the enrichment cultures. Overall, this study demonstrates that microbes from anaerobic lake sediments can methylate (and demethylate) arsenic, a capability shared by manganese−, iron−, and sulfate–reducing microbial consortia. |
format |
Article in Journal/Newspaper |
author |
Bright, D. A. Brock, S. Reimer, K. J. Cullen, W. R. Hewitt, G. M. Jafaar, J. |
spellingShingle |
Bright, D. A. Brock, S. Reimer, K. J. Cullen, W. R. Hewitt, G. M. Jafaar, J. Methylation of arsenic by anaerobic microbial consortia isolated from lake sediment |
author_facet |
Bright, D. A. Brock, S. Reimer, K. J. Cullen, W. R. Hewitt, G. M. Jafaar, J. |
author_sort |
Bright, D. A. |
title |
Methylation of arsenic by anaerobic microbial consortia isolated from lake sediment |
title_short |
Methylation of arsenic by anaerobic microbial consortia isolated from lake sediment |
title_full |
Methylation of arsenic by anaerobic microbial consortia isolated from lake sediment |
title_fullStr |
Methylation of arsenic by anaerobic microbial consortia isolated from lake sediment |
title_full_unstemmed |
Methylation of arsenic by anaerobic microbial consortia isolated from lake sediment |
title_sort |
methylation of arsenic by anaerobic microbial consortia isolated from lake sediment |
publisher |
Wiley |
publishDate |
1994 |
url |
http://dx.doi.org/10.1002/aoc.590080416 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Faoc.590080416 https://onlinelibrary.wiley.com/doi/pdf/10.1002/aoc.590080416 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Applied Organometallic Chemistry volume 8, issue 4, page 415-422 ISSN 0268-2605 1099-0739 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/aoc.590080416 |
container_title |
Applied Organometallic Chemistry |
container_volume |
8 |
container_issue |
4 |
container_start_page |
415 |
op_container_end_page |
422 |
_version_ |
1809894755112321024 |