Integrating Enzymes with Supramolecular Polymers for Recyclable Photobiocatalytic Catalysis
Abstract Chemical modifications of enzymes excel in the realm of enzyme engineering due to its directness, robustness, and efficiency; however, challenges persist in devising versatile and effective strategies. In this study, we introduce a supramolecular modification methodology that amalgamates a...
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Online Access: | http://dx.doi.org/10.1002/ange.202400105 https://onlinelibrary.wiley.com/doi/pdf/10.1002/ange.202400105 |
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crwiley:10.1002/ange.202400105 2024-06-02T07:58:07+00:00 Integrating Enzymes with Supramolecular Polymers for Recyclable Photobiocatalytic Catalysis Ouyang, Jingping Zhang, Zhenfang Li, Jian Wu, Changzhu Danmarks Frie Forskningsfond Novo Nordisk Fonden Carlsbergfondet 2024 http://dx.doi.org/10.1002/ange.202400105 https://onlinelibrary.wiley.com/doi/pdf/10.1002/ange.202400105 en eng Wiley http://creativecommons.org/licenses/by/4.0/ Angewandte Chemie volume 136, issue 16 ISSN 0044-8249 1521-3757 journal-article 2024 crwiley https://doi.org/10.1002/ange.202400105 2024-05-03T11:11:31Z Abstract Chemical modifications of enzymes excel in the realm of enzyme engineering due to its directness, robustness, and efficiency; however, challenges persist in devising versatile and effective strategies. In this study, we introduce a supramolecular modification methodology that amalgamates a supramolecular polymer with Candida antarctica lipase B (CalB) to create supramolecular enzymes (SupEnzyme). This approach features the straightforward preparation of a supramolecular amphiphilic polymer (β‐CD@SMA), which was subsequently conjugated to the enzyme, resulting in a SupEnzyme capable of self‐assembly into supramolecular nanoparticles. The resulting SupEnzyme nanoparticles can form micron‐scale supramolecular aggregates through supramolecular and electrostatic interactions with guest entities, thus enhancing catalyst recycling. Remarkably, these aggregates maintain 80 % activity after seven cycles, outperforming Novozym 435. Additionally, they can effectively initiate photobiocatalytic cascade reactions using guest photocatalysts. As a consequence, our SupEnzyme methodology exhibits noteworthy adaptability in enzyme modification, presenting a versatile platform for various polymer, enzyme, and biocompatible catalyst pairings, with potential applications in the fields of chemistry and biology. Article in Journal/Newspaper Antarc* Antarctica Wiley Online Library Angewandte Chemie |
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Wiley Online Library |
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English |
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Abstract Chemical modifications of enzymes excel in the realm of enzyme engineering due to its directness, robustness, and efficiency; however, challenges persist in devising versatile and effective strategies. In this study, we introduce a supramolecular modification methodology that amalgamates a supramolecular polymer with Candida antarctica lipase B (CalB) to create supramolecular enzymes (SupEnzyme). This approach features the straightforward preparation of a supramolecular amphiphilic polymer (β‐CD@SMA), which was subsequently conjugated to the enzyme, resulting in a SupEnzyme capable of self‐assembly into supramolecular nanoparticles. The resulting SupEnzyme nanoparticles can form micron‐scale supramolecular aggregates through supramolecular and electrostatic interactions with guest entities, thus enhancing catalyst recycling. Remarkably, these aggregates maintain 80 % activity after seven cycles, outperforming Novozym 435. Additionally, they can effectively initiate photobiocatalytic cascade reactions using guest photocatalysts. As a consequence, our SupEnzyme methodology exhibits noteworthy adaptability in enzyme modification, presenting a versatile platform for various polymer, enzyme, and biocompatible catalyst pairings, with potential applications in the fields of chemistry and biology. |
author2 |
Danmarks Frie Forskningsfond Novo Nordisk Fonden Carlsbergfondet |
format |
Article in Journal/Newspaper |
author |
Ouyang, Jingping Zhang, Zhenfang Li, Jian Wu, Changzhu |
spellingShingle |
Ouyang, Jingping Zhang, Zhenfang Li, Jian Wu, Changzhu Integrating Enzymes with Supramolecular Polymers for Recyclable Photobiocatalytic Catalysis |
author_facet |
Ouyang, Jingping Zhang, Zhenfang Li, Jian Wu, Changzhu |
author_sort |
Ouyang, Jingping |
title |
Integrating Enzymes with Supramolecular Polymers for Recyclable Photobiocatalytic Catalysis |
title_short |
Integrating Enzymes with Supramolecular Polymers for Recyclable Photobiocatalytic Catalysis |
title_full |
Integrating Enzymes with Supramolecular Polymers for Recyclable Photobiocatalytic Catalysis |
title_fullStr |
Integrating Enzymes with Supramolecular Polymers for Recyclable Photobiocatalytic Catalysis |
title_full_unstemmed |
Integrating Enzymes with Supramolecular Polymers for Recyclable Photobiocatalytic Catalysis |
title_sort |
integrating enzymes with supramolecular polymers for recyclable photobiocatalytic catalysis |
publisher |
Wiley |
publishDate |
2024 |
url |
http://dx.doi.org/10.1002/ange.202400105 https://onlinelibrary.wiley.com/doi/pdf/10.1002/ange.202400105 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Angewandte Chemie volume 136, issue 16 ISSN 0044-8249 1521-3757 |
op_rights |
http://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1002/ange.202400105 |
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Angewandte Chemie |
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