A preliminary analysis of the DNA and diet of the extinct Beothuk: A systematic approach to ancient human DNA
Abstract We have used a systematic protocol for extracting, quantitating, sexing and validating ancient human mitochondrial and nuclear DNA of one male and one female Beothuk, a Native American population from Newfoundland, which became extinct ∼180 years ago. They carried mtDNA haplotypes, which fa...
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crwiley:10.1002/ajpa.20536 2024-06-02T08:04:17+00:00 A preliminary analysis of the DNA and diet of the extinct Beothuk: A systematic approach to ancient human DNA Kuch, Melanie Gröcke, Darren R. Knyf, Martin C. Gilbert, M. Thomas P. Younghusband, Ban Young, Terry Marshall, Ingeborg Willerslev, Eske Stoneking, Mark Poinar, Hendrik 2007 http://dx.doi.org/10.1002/ajpa.20536 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fajpa.20536 https://onlinelibrary.wiley.com/doi/pdf/10.1002/ajpa.20536 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor American Journal of Physical Anthropology volume 132, issue 4, page 594-604 ISSN 0002-9483 1096-8644 journal-article 2007 crwiley https://doi.org/10.1002/ajpa.20536 2024-05-03T10:37:22Z Abstract We have used a systematic protocol for extracting, quantitating, sexing and validating ancient human mitochondrial and nuclear DNA of one male and one female Beothuk, a Native American population from Newfoundland, which became extinct ∼180 years ago. They carried mtDNA haplotypes, which fall within haplogroups X and C, consistent with Northeastern Native populations today. In addition we have sexed the male using a novel‐sexing assay and confirmed the authenticity of his Y chromosome with the presence of the Native American specific Y‐QM3 single nucleotide polymorphism (SNP). This is the first ancient nuclear SNP typed from a Native population in the Americas. In addition, using the same teeth we conducted a stable isotopes analysis of collagen and dentine to show that both individuals relied on marine sources (fresh and salt water fish, seals) with no hierarchy seen between them, and that their water sources were pooled or stored water. Both mtDNA sequence data and Y SNP data hint at possible gene flow or a common ancestral population for both the Beothuk and the current day Mikmaq, but more importantly the data do not lend credence to the proposed idea that the Beothuk (specifically, Nonosabasut) were of admixed (European‐Native American) descent. We also analyzed patterns of DNA damage in the clones of authentic mtDNA sequences; there is no tendency for DNA damage to occur preferentially at previously defined mutational hotspots, suggesting that such mutational hotspots are not hypervariable because they are more prone to damage. Am J Phys Anthropol, 2007. © 2007 Wiley‐Liss, Inc. Article in Journal/Newspaper Beothuk Newfoundland Wiley Online Library American Journal of Physical Anthropology 132 4 594 604 |
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Wiley Online Library |
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English |
description |
Abstract We have used a systematic protocol for extracting, quantitating, sexing and validating ancient human mitochondrial and nuclear DNA of one male and one female Beothuk, a Native American population from Newfoundland, which became extinct ∼180 years ago. They carried mtDNA haplotypes, which fall within haplogroups X and C, consistent with Northeastern Native populations today. In addition we have sexed the male using a novel‐sexing assay and confirmed the authenticity of his Y chromosome with the presence of the Native American specific Y‐QM3 single nucleotide polymorphism (SNP). This is the first ancient nuclear SNP typed from a Native population in the Americas. In addition, using the same teeth we conducted a stable isotopes analysis of collagen and dentine to show that both individuals relied on marine sources (fresh and salt water fish, seals) with no hierarchy seen between them, and that their water sources were pooled or stored water. Both mtDNA sequence data and Y SNP data hint at possible gene flow or a common ancestral population for both the Beothuk and the current day Mikmaq, but more importantly the data do not lend credence to the proposed idea that the Beothuk (specifically, Nonosabasut) were of admixed (European‐Native American) descent. We also analyzed patterns of DNA damage in the clones of authentic mtDNA sequences; there is no tendency for DNA damage to occur preferentially at previously defined mutational hotspots, suggesting that such mutational hotspots are not hypervariable because they are more prone to damage. Am J Phys Anthropol, 2007. © 2007 Wiley‐Liss, Inc. |
format |
Article in Journal/Newspaper |
author |
Kuch, Melanie Gröcke, Darren R. Knyf, Martin C. Gilbert, M. Thomas P. Younghusband, Ban Young, Terry Marshall, Ingeborg Willerslev, Eske Stoneking, Mark Poinar, Hendrik |
spellingShingle |
Kuch, Melanie Gröcke, Darren R. Knyf, Martin C. Gilbert, M. Thomas P. Younghusband, Ban Young, Terry Marshall, Ingeborg Willerslev, Eske Stoneking, Mark Poinar, Hendrik A preliminary analysis of the DNA and diet of the extinct Beothuk: A systematic approach to ancient human DNA |
author_facet |
Kuch, Melanie Gröcke, Darren R. Knyf, Martin C. Gilbert, M. Thomas P. Younghusband, Ban Young, Terry Marshall, Ingeborg Willerslev, Eske Stoneking, Mark Poinar, Hendrik |
author_sort |
Kuch, Melanie |
title |
A preliminary analysis of the DNA and diet of the extinct Beothuk: A systematic approach to ancient human DNA |
title_short |
A preliminary analysis of the DNA and diet of the extinct Beothuk: A systematic approach to ancient human DNA |
title_full |
A preliminary analysis of the DNA and diet of the extinct Beothuk: A systematic approach to ancient human DNA |
title_fullStr |
A preliminary analysis of the DNA and diet of the extinct Beothuk: A systematic approach to ancient human DNA |
title_full_unstemmed |
A preliminary analysis of the DNA and diet of the extinct Beothuk: A systematic approach to ancient human DNA |
title_sort |
preliminary analysis of the dna and diet of the extinct beothuk: a systematic approach to ancient human dna |
publisher |
Wiley |
publishDate |
2007 |
url |
http://dx.doi.org/10.1002/ajpa.20536 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fajpa.20536 https://onlinelibrary.wiley.com/doi/pdf/10.1002/ajpa.20536 |
genre |
Beothuk Newfoundland |
genre_facet |
Beothuk Newfoundland |
op_source |
American Journal of Physical Anthropology volume 132, issue 4, page 594-604 ISSN 0002-9483 1096-8644 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/ajpa.20536 |
container_title |
American Journal of Physical Anthropology |
container_volume |
132 |
container_issue |
4 |
container_start_page |
594 |
op_container_end_page |
604 |
_version_ |
1800748919147200512 |