RNAlater facilitates remote sampling of aquaculture Atlantic salmon liver for proteomic analysis
Abstract During sample collection and transport, high‐quality nucleic acids, proteins and metabolites are preserved by snap‐freezing (SF) samples in liquid nitrogen or dry ice. In remote aquaculture facilities, SF materials are not readily available and can be hazardous during sampling and transport...
Published in: | Aquaculture, Fish and Fisheries |
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crwiley:10.1002/aff2.73 2024-06-02T08:03:40+00:00 RNAlater facilitates remote sampling of aquaculture Atlantic salmon liver for proteomic analysis Rusu, Anca G. Broadbent, James A. Wade, Nicholas M. Simon, Cedric J. Rombenso, Artur N. Osborne, Simone A. Mendoza‐Porras, Omar Commonwealth Scientific and Industrial Research Organisation 2022 http://dx.doi.org/10.1002/aff2.73 https://onlinelibrary.wiley.com/doi/pdf/10.1002/aff2.73 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/aff2.73 en eng Wiley http://creativecommons.org/licenses/by/4.0/ Aquaculture, Fish and Fisheries volume 2, issue 6, page 578-586 ISSN 2693-8847 2693-8847 journal-article 2022 crwiley https://doi.org/10.1002/aff2.73 2024-05-03T11:13:12Z Abstract During sample collection and transport, high‐quality nucleic acids, proteins and metabolites are preserved by snap‐freezing (SF) samples in liquid nitrogen or dry ice. In remote aquaculture facilities, SF materials are not readily available and can be hazardous during sampling and transport. RNAlater is widely used in aquaculture for transcriptome studies, but its effect on proteome stability needs further investigation. Here, we used proteomics to demonstrate that RNAlater (RL) preserved liver samples similarly to SF samples. Additionally, 69 proteins (∼2.6%) comprising ribosomal proteins, transcription cofactors, translational factors, proteases and transmembrane proteins were significantly less abundant in the RL proteome. These proteins are expected to be denatured by RL and are cellular components of ribosomes and nucleosomes and involved in proteolysis, transcription and translation. We also demonstrated that RL did not influence abundance of important proteins originally identified in liver samples from salmon reared under heat stress, hypoxia or specific feeding regimes. These findings suggest that preserving samples in RL is suitable for proteomics and offer a safer and effective preservation technology for use in remote aquaculture locations. Article in Journal/Newspaper Atlantic salmon Wiley Online Library Aquaculture, Fish and Fisheries 2 6 578 586 |
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Wiley Online Library |
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English |
description |
Abstract During sample collection and transport, high‐quality nucleic acids, proteins and metabolites are preserved by snap‐freezing (SF) samples in liquid nitrogen or dry ice. In remote aquaculture facilities, SF materials are not readily available and can be hazardous during sampling and transport. RNAlater is widely used in aquaculture for transcriptome studies, but its effect on proteome stability needs further investigation. Here, we used proteomics to demonstrate that RNAlater (RL) preserved liver samples similarly to SF samples. Additionally, 69 proteins (∼2.6%) comprising ribosomal proteins, transcription cofactors, translational factors, proteases and transmembrane proteins were significantly less abundant in the RL proteome. These proteins are expected to be denatured by RL and are cellular components of ribosomes and nucleosomes and involved in proteolysis, transcription and translation. We also demonstrated that RL did not influence abundance of important proteins originally identified in liver samples from salmon reared under heat stress, hypoxia or specific feeding regimes. These findings suggest that preserving samples in RL is suitable for proteomics and offer a safer and effective preservation technology for use in remote aquaculture locations. |
author2 |
Commonwealth Scientific and Industrial Research Organisation |
format |
Article in Journal/Newspaper |
author |
Rusu, Anca G. Broadbent, James A. Wade, Nicholas M. Simon, Cedric J. Rombenso, Artur N. Osborne, Simone A. Mendoza‐Porras, Omar |
spellingShingle |
Rusu, Anca G. Broadbent, James A. Wade, Nicholas M. Simon, Cedric J. Rombenso, Artur N. Osborne, Simone A. Mendoza‐Porras, Omar RNAlater facilitates remote sampling of aquaculture Atlantic salmon liver for proteomic analysis |
author_facet |
Rusu, Anca G. Broadbent, James A. Wade, Nicholas M. Simon, Cedric J. Rombenso, Artur N. Osborne, Simone A. Mendoza‐Porras, Omar |
author_sort |
Rusu, Anca G. |
title |
RNAlater facilitates remote sampling of aquaculture Atlantic salmon liver for proteomic analysis |
title_short |
RNAlater facilitates remote sampling of aquaculture Atlantic salmon liver for proteomic analysis |
title_full |
RNAlater facilitates remote sampling of aquaculture Atlantic salmon liver for proteomic analysis |
title_fullStr |
RNAlater facilitates remote sampling of aquaculture Atlantic salmon liver for proteomic analysis |
title_full_unstemmed |
RNAlater facilitates remote sampling of aquaculture Atlantic salmon liver for proteomic analysis |
title_sort |
rnalater facilitates remote sampling of aquaculture atlantic salmon liver for proteomic analysis |
publisher |
Wiley |
publishDate |
2022 |
url |
http://dx.doi.org/10.1002/aff2.73 https://onlinelibrary.wiley.com/doi/pdf/10.1002/aff2.73 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/aff2.73 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
Aquaculture, Fish and Fisheries volume 2, issue 6, page 578-586 ISSN 2693-8847 2693-8847 |
op_rights |
http://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1002/aff2.73 |
container_title |
Aquaculture, Fish and Fisheries |
container_volume |
2 |
container_issue |
6 |
container_start_page |
578 |
op_container_end_page |
586 |
_version_ |
1800748256210190336 |