Rapid detection of heat stress biomarkers in Atlantic salmon ( Salmo salar) liver using targeted proteomics
Abstract Most fish are ectothermic; therefore, their physiology is significantly affected by temperature. Aquaculture fish have limited ability to avoid elevated water temperatures, with impacts increasing as a result of climate change. To date, quantifying gene expression has been proposed to monit...
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crwiley:10.1002/aff2.147 2024-06-02T08:03:43+00:00 Rapid detection of heat stress biomarkers in Atlantic salmon ( Salmo salar) liver using targeted proteomics Mendoza‐Porras, Omar Rusu, Anca G. Stratford, Christopher Wade, Nicholas M. Cooperative Research Centres, Australian Government Department of Industry 2023 http://dx.doi.org/10.1002/aff2.147 https://onlinelibrary.wiley.com/doi/pdf/10.1002/aff2.147 en eng Wiley http://creativecommons.org/licenses/by/4.0/ Aquaculture, Fish and Fisheries volume 4, issue 1 ISSN 2693-8847 2693-8847 journal-article 2023 crwiley https://doi.org/10.1002/aff2.147 2024-05-03T10:51:35Z Abstract Most fish are ectothermic; therefore, their physiology is significantly affected by temperature. Aquaculture fish have limited ability to avoid elevated water temperatures, with impacts increasing as a result of climate change. To date, quantifying gene expression has been proposed to monitor heat stress in salmon liver. This study aimed to establish a faster multiplexed proteomics method to measure the abundance of thermal stress biomarkers in liver of salmon reared at 15°C or 20°C. Moreover, this study aimed to determine the effects that sample pooling, and data normalisation using housekeeping (HK) protein peptides would exert over the statistical significance of these thermal stress markers. A multiple reaction monitoring (MRM) mass spectrometry method, comprised 45 peptides derived from thermal stress markers and 10 peptides from HK proteins, was applied to measure these markers in liver of salmon reared at 15°C or 20°C. When samples were processed individually, 34 peptides were significant between salmon livers at 15°C or 20°C. In pooled samples, this decreased to five significant peptides. Peptides hprt1_HYADDLDR (hypoxanthine phosphoribosyl transferase) and gapdh_VPTPNVSVVDLTVR (glyceraldehyde‐3‐phosphate dehydrogenase) were the most stable and unstable HK protein peptides, respectively. When data was normalised with hprt1_HYADDLDR, 16 peptides were significant in individual samples and 13 in pooled samples. Significant peptides serpinh1a_ADLSNISGK, SerpinH1_TNSILFIGR, ela2_VVGGEDVR and gapdh_VPTPNVSVVDLTVR were common regardless of data strategy. A fast and reliable MRM method was established to validate thermal stress markers in salmon liver, where individual samples yielded better results than pooled samples. Sample pooling was only better when combined with normalisation as it validated twice the number of markers than sample pooling alone. This method could be applied to monitoring stress response in experiments involving feeding additives designed to mitigate thermal stress or in selective ... Article in Journal/Newspaper Atlantic salmon Salmo salar Wiley Online Library Aquaculture, Fish and Fisheries 4 1 |
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crwiley |
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English |
description |
Abstract Most fish are ectothermic; therefore, their physiology is significantly affected by temperature. Aquaculture fish have limited ability to avoid elevated water temperatures, with impacts increasing as a result of climate change. To date, quantifying gene expression has been proposed to monitor heat stress in salmon liver. This study aimed to establish a faster multiplexed proteomics method to measure the abundance of thermal stress biomarkers in liver of salmon reared at 15°C or 20°C. Moreover, this study aimed to determine the effects that sample pooling, and data normalisation using housekeeping (HK) protein peptides would exert over the statistical significance of these thermal stress markers. A multiple reaction monitoring (MRM) mass spectrometry method, comprised 45 peptides derived from thermal stress markers and 10 peptides from HK proteins, was applied to measure these markers in liver of salmon reared at 15°C or 20°C. When samples were processed individually, 34 peptides were significant between salmon livers at 15°C or 20°C. In pooled samples, this decreased to five significant peptides. Peptides hprt1_HYADDLDR (hypoxanthine phosphoribosyl transferase) and gapdh_VPTPNVSVVDLTVR (glyceraldehyde‐3‐phosphate dehydrogenase) were the most stable and unstable HK protein peptides, respectively. When data was normalised with hprt1_HYADDLDR, 16 peptides were significant in individual samples and 13 in pooled samples. Significant peptides serpinh1a_ADLSNISGK, SerpinH1_TNSILFIGR, ela2_VVGGEDVR and gapdh_VPTPNVSVVDLTVR were common regardless of data strategy. A fast and reliable MRM method was established to validate thermal stress markers in salmon liver, where individual samples yielded better results than pooled samples. Sample pooling was only better when combined with normalisation as it validated twice the number of markers than sample pooling alone. This method could be applied to monitoring stress response in experiments involving feeding additives designed to mitigate thermal stress or in selective ... |
author2 |
Cooperative Research Centres, Australian Government Department of Industry |
format |
Article in Journal/Newspaper |
author |
Mendoza‐Porras, Omar Rusu, Anca G. Stratford, Christopher Wade, Nicholas M. |
spellingShingle |
Mendoza‐Porras, Omar Rusu, Anca G. Stratford, Christopher Wade, Nicholas M. Rapid detection of heat stress biomarkers in Atlantic salmon ( Salmo salar) liver using targeted proteomics |
author_facet |
Mendoza‐Porras, Omar Rusu, Anca G. Stratford, Christopher Wade, Nicholas M. |
author_sort |
Mendoza‐Porras, Omar |
title |
Rapid detection of heat stress biomarkers in Atlantic salmon ( Salmo salar) liver using targeted proteomics |
title_short |
Rapid detection of heat stress biomarkers in Atlantic salmon ( Salmo salar) liver using targeted proteomics |
title_full |
Rapid detection of heat stress biomarkers in Atlantic salmon ( Salmo salar) liver using targeted proteomics |
title_fullStr |
Rapid detection of heat stress biomarkers in Atlantic salmon ( Salmo salar) liver using targeted proteomics |
title_full_unstemmed |
Rapid detection of heat stress biomarkers in Atlantic salmon ( Salmo salar) liver using targeted proteomics |
title_sort |
rapid detection of heat stress biomarkers in atlantic salmon ( salmo salar) liver using targeted proteomics |
publisher |
Wiley |
publishDate |
2023 |
url |
http://dx.doi.org/10.1002/aff2.147 https://onlinelibrary.wiley.com/doi/pdf/10.1002/aff2.147 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Aquaculture, Fish and Fisheries volume 4, issue 1 ISSN 2693-8847 2693-8847 |
op_rights |
http://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1002/aff2.147 |
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Aquaculture, Fish and Fisheries |
container_volume |
4 |
container_issue |
1 |
_version_ |
1800748320415547392 |