Characterization of Common Minke Whale ( Balaenoptera Acutorostrata) Cell Lines Immortalized with the Expression of Cell Cycle Regulators
Abstract Primary cultured cells cannot proliferate infinite. The overcoming of this limit can be classified as immortalization. Bypass of p16 senescence protein induces efficient immortalization various types of mammalians is previously reported. However, the Cetacea species is not known. Here, that...
Published in: | Advanced Biology |
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Language: | English |
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Online Access: | http://dx.doi.org/10.1002/adbi.202300227 https://onlinelibrary.wiley.com/doi/pdf/10.1002/adbi.202300227 |
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crwiley:10.1002/adbi.202300227 2024-06-02T08:03:54+00:00 Characterization of Common Minke Whale ( Balaenoptera Acutorostrata) Cell Lines Immortalized with the Expression of Cell Cycle Regulators Sekine, Aya Yasunaga, Genta Kumamoto, Soichiro Fujibayashi, So Munirah, Izzah Bai, Lanlan Tani, Tetsuya Sugano, Eriko Tomita, Hiroshi Ozaki, Taku Kiyono, Tohru Inoue‐Murayama, Miho Fukuda, Tomokazu Japan Society for the Promotion of Science NODAI Genome Research Center, Tokyo University of Agriculture Iwate University 2023 http://dx.doi.org/10.1002/adbi.202300227 https://onlinelibrary.wiley.com/doi/pdf/10.1002/adbi.202300227 en eng Wiley http://creativecommons.org/licenses/by/4.0/ Advanced Biology volume 8, issue 3 ISSN 2701-0198 2701-0198 journal-article 2023 crwiley https://doi.org/10.1002/adbi.202300227 2024-05-03T11:40:06Z Abstract Primary cultured cells cannot proliferate infinite. The overcoming of this limit can be classified as immortalization. Bypass of p16 senescence protein induces efficient immortalization various types of mammalians is previously reported. However, the Cetacea species is not known. Here, that common minke whale‐derived cells can be immortalized with a combination of human genes, mutant cyclin‐dependent kinase 4 (CDK4 R24C ), cyclin D1, and Telomerase Reverse Transcriptase (TERT) is reported. These results indicate that the function of cell cycle regulators in premature senescence is evolutionarily conserved. This study describes the conserved roles of cell cycle regulators in the immortalization of cells from humans to Cetacea species. Furthermore, using RNA‐seq based on next‐generation sequencing, the gene expression profiles of immortalized cells are compared with parental cells as well as those immortalized with SV40 large T antigen, which is once a popular method for cellular immortalization. The profiling results show that newly established common minke‐whale‐derived immortaliozed cells have completely different profiles from SV40 cells. This result indicates that the expression of mutant CDK4, cyclin D1, and TERT enables to establish immortalized cell lines with different biological nature from SV40 expressing cells. Article in Journal/Newspaper Balaenoptera acutorostrata minke whale Wiley Online Library Advanced Biology |
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Open Polar |
collection |
Wiley Online Library |
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crwiley |
language |
English |
description |
Abstract Primary cultured cells cannot proliferate infinite. The overcoming of this limit can be classified as immortalization. Bypass of p16 senescence protein induces efficient immortalization various types of mammalians is previously reported. However, the Cetacea species is not known. Here, that common minke whale‐derived cells can be immortalized with a combination of human genes, mutant cyclin‐dependent kinase 4 (CDK4 R24C ), cyclin D1, and Telomerase Reverse Transcriptase (TERT) is reported. These results indicate that the function of cell cycle regulators in premature senescence is evolutionarily conserved. This study describes the conserved roles of cell cycle regulators in the immortalization of cells from humans to Cetacea species. Furthermore, using RNA‐seq based on next‐generation sequencing, the gene expression profiles of immortalized cells are compared with parental cells as well as those immortalized with SV40 large T antigen, which is once a popular method for cellular immortalization. The profiling results show that newly established common minke‐whale‐derived immortaliozed cells have completely different profiles from SV40 cells. This result indicates that the expression of mutant CDK4, cyclin D1, and TERT enables to establish immortalized cell lines with different biological nature from SV40 expressing cells. |
author2 |
Japan Society for the Promotion of Science NODAI Genome Research Center, Tokyo University of Agriculture Iwate University |
format |
Article in Journal/Newspaper |
author |
Sekine, Aya Yasunaga, Genta Kumamoto, Soichiro Fujibayashi, So Munirah, Izzah Bai, Lanlan Tani, Tetsuya Sugano, Eriko Tomita, Hiroshi Ozaki, Taku Kiyono, Tohru Inoue‐Murayama, Miho Fukuda, Tomokazu |
spellingShingle |
Sekine, Aya Yasunaga, Genta Kumamoto, Soichiro Fujibayashi, So Munirah, Izzah Bai, Lanlan Tani, Tetsuya Sugano, Eriko Tomita, Hiroshi Ozaki, Taku Kiyono, Tohru Inoue‐Murayama, Miho Fukuda, Tomokazu Characterization of Common Minke Whale ( Balaenoptera Acutorostrata) Cell Lines Immortalized with the Expression of Cell Cycle Regulators |
author_facet |
Sekine, Aya Yasunaga, Genta Kumamoto, Soichiro Fujibayashi, So Munirah, Izzah Bai, Lanlan Tani, Tetsuya Sugano, Eriko Tomita, Hiroshi Ozaki, Taku Kiyono, Tohru Inoue‐Murayama, Miho Fukuda, Tomokazu |
author_sort |
Sekine, Aya |
title |
Characterization of Common Minke Whale ( Balaenoptera Acutorostrata) Cell Lines Immortalized with the Expression of Cell Cycle Regulators |
title_short |
Characterization of Common Minke Whale ( Balaenoptera Acutorostrata) Cell Lines Immortalized with the Expression of Cell Cycle Regulators |
title_full |
Characterization of Common Minke Whale ( Balaenoptera Acutorostrata) Cell Lines Immortalized with the Expression of Cell Cycle Regulators |
title_fullStr |
Characterization of Common Minke Whale ( Balaenoptera Acutorostrata) Cell Lines Immortalized with the Expression of Cell Cycle Regulators |
title_full_unstemmed |
Characterization of Common Minke Whale ( Balaenoptera Acutorostrata) Cell Lines Immortalized with the Expression of Cell Cycle Regulators |
title_sort |
characterization of common minke whale ( balaenoptera acutorostrata) cell lines immortalized with the expression of cell cycle regulators |
publisher |
Wiley |
publishDate |
2023 |
url |
http://dx.doi.org/10.1002/adbi.202300227 https://onlinelibrary.wiley.com/doi/pdf/10.1002/adbi.202300227 |
genre |
Balaenoptera acutorostrata minke whale |
genre_facet |
Balaenoptera acutorostrata minke whale |
op_source |
Advanced Biology volume 8, issue 3 ISSN 2701-0198 2701-0198 |
op_rights |
http://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1002/adbi.202300227 |
container_title |
Advanced Biology |
_version_ |
1800748507004403712 |