The gastric mucosa of Atlantic salmon ( Salmo salar) is abundant in highly active chitinases
Atlantic salmon ( Salmo salar ) possesses a genome containing 10 genes encoding chitinases, yet their functional roles remain poorly understood. In other fish species, chitinases have been primarily linked to digestion, but also to other functions, as chitinase‐encoding genes are transcribed in a va...
Published in: | FEBS Open Bio |
---|---|
Main Authors: | , , , |
Other Authors: | , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Wiley
2023
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1002/2211-5463.13694 https://febs.onlinelibrary.wiley.com/doi/pdf/10.1002/2211-5463.13694 |
id |
crwiley:10.1002/2211-5463.13694 |
---|---|
record_format |
openpolar |
spelling |
crwiley:10.1002/2211-5463.13694 2024-06-02T08:03:26+00:00 The gastric mucosa of Atlantic salmon ( Salmo salar) is abundant in highly active chitinases Mengkrog Holen, Matilde Tuveng, Tina Rise Kent, Matthew Peter Vaaje‐Kolstad, Gustav Norges Miljø- og Biovitenskapelige Universitet Norges Forskningsråd 2023 http://dx.doi.org/10.1002/2211-5463.13694 https://febs.onlinelibrary.wiley.com/doi/pdf/10.1002/2211-5463.13694 en eng Wiley http://creativecommons.org/licenses/by/4.0/ FEBS Open Bio volume 14, issue 1, page 23-36 ISSN 2211-5463 2211-5463 journal-article 2023 crwiley https://doi.org/10.1002/2211-5463.13694 2024-05-03T11:43:25Z Atlantic salmon ( Salmo salar ) possesses a genome containing 10 genes encoding chitinases, yet their functional roles remain poorly understood. In other fish species, chitinases have been primarily linked to digestion, but also to other functions, as chitinase‐encoding genes are transcribed in a variety of non‐digestive organs. In this study, we investigated the properties of two chitinases belonging to the family 18 glycoside hydrolase group, namely Chia.3 and Chia.4, both isolated from the stomach mucosa. Chia.3 and Chia.4, exhibiting 95% sequence identity, proved inseparable using conventional chromatographic methods, necessitating their purification as a chitinase pair. Biochemical analysis revealed sustained chitinolytic activity against β‐chitin for up to 24 h, spanning a pH range of 2 to 6. Moreover, subsequent in vitro investigations established that this chitinase pair efficiently degrades diverse chitin‐containing substrates into chitobiose, highlighting the potential of Atlantic salmon to utilize novel chitin‐containing feed sources. Analysis of the gastric matrix proteome demonstrates that the chitinases are secreted and rank among the most abundant proteins in the gastric matrix. This finding correlates well with the previously observed high transcription of the corresponding chitinase genes in Atlantic salmon stomach tissue. By shedding light on the secreted chitinases in the Atlantic salmon's stomach mucosa and elucidating their functional characteristics, this study enhances our understanding of chitinase biology in this species. Moreover, the observed capacity to effectively degrade chitin‐containing materials implies the potential utilization of alternative feed sources rich in chitin, offering promising prospects for sustainable aquaculture practices. Article in Journal/Newspaper Atlantic salmon Salmo salar Wiley Online Library FEBS Open Bio |
institution |
Open Polar |
collection |
Wiley Online Library |
op_collection_id |
crwiley |
language |
English |
description |
Atlantic salmon ( Salmo salar ) possesses a genome containing 10 genes encoding chitinases, yet their functional roles remain poorly understood. In other fish species, chitinases have been primarily linked to digestion, but also to other functions, as chitinase‐encoding genes are transcribed in a variety of non‐digestive organs. In this study, we investigated the properties of two chitinases belonging to the family 18 glycoside hydrolase group, namely Chia.3 and Chia.4, both isolated from the stomach mucosa. Chia.3 and Chia.4, exhibiting 95% sequence identity, proved inseparable using conventional chromatographic methods, necessitating their purification as a chitinase pair. Biochemical analysis revealed sustained chitinolytic activity against β‐chitin for up to 24 h, spanning a pH range of 2 to 6. Moreover, subsequent in vitro investigations established that this chitinase pair efficiently degrades diverse chitin‐containing substrates into chitobiose, highlighting the potential of Atlantic salmon to utilize novel chitin‐containing feed sources. Analysis of the gastric matrix proteome demonstrates that the chitinases are secreted and rank among the most abundant proteins in the gastric matrix. This finding correlates well with the previously observed high transcription of the corresponding chitinase genes in Atlantic salmon stomach tissue. By shedding light on the secreted chitinases in the Atlantic salmon's stomach mucosa and elucidating their functional characteristics, this study enhances our understanding of chitinase biology in this species. Moreover, the observed capacity to effectively degrade chitin‐containing materials implies the potential utilization of alternative feed sources rich in chitin, offering promising prospects for sustainable aquaculture practices. |
author2 |
Norges Miljø- og Biovitenskapelige Universitet Norges Forskningsråd |
format |
Article in Journal/Newspaper |
author |
Mengkrog Holen, Matilde Tuveng, Tina Rise Kent, Matthew Peter Vaaje‐Kolstad, Gustav |
spellingShingle |
Mengkrog Holen, Matilde Tuveng, Tina Rise Kent, Matthew Peter Vaaje‐Kolstad, Gustav The gastric mucosa of Atlantic salmon ( Salmo salar) is abundant in highly active chitinases |
author_facet |
Mengkrog Holen, Matilde Tuveng, Tina Rise Kent, Matthew Peter Vaaje‐Kolstad, Gustav |
author_sort |
Mengkrog Holen, Matilde |
title |
The gastric mucosa of Atlantic salmon ( Salmo salar) is abundant in highly active chitinases |
title_short |
The gastric mucosa of Atlantic salmon ( Salmo salar) is abundant in highly active chitinases |
title_full |
The gastric mucosa of Atlantic salmon ( Salmo salar) is abundant in highly active chitinases |
title_fullStr |
The gastric mucosa of Atlantic salmon ( Salmo salar) is abundant in highly active chitinases |
title_full_unstemmed |
The gastric mucosa of Atlantic salmon ( Salmo salar) is abundant in highly active chitinases |
title_sort |
gastric mucosa of atlantic salmon ( salmo salar) is abundant in highly active chitinases |
publisher |
Wiley |
publishDate |
2023 |
url |
http://dx.doi.org/10.1002/2211-5463.13694 https://febs.onlinelibrary.wiley.com/doi/pdf/10.1002/2211-5463.13694 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
FEBS Open Bio volume 14, issue 1, page 23-36 ISSN 2211-5463 2211-5463 |
op_rights |
http://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1002/2211-5463.13694 |
container_title |
FEBS Open Bio |
_version_ |
1800747946392682496 |