Mechanisms to Explain the Elemental Composition of the Initial Aragonite Shell of Larval Oysters
Abstract Calcifying organisms face increasing stress from the changing carbonate chemistry of an acidifying ocean, particularly bivalve larvae that live in upwelling regions of the world, such as the coastal and estuarine waters of Oregon (USA). Arguably the first and most significant developmental...
Published in: | Geochemistry, Geophysics, Geosystems |
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crwiley:10.1002/2017gc007133 2024-09-15T18:03:16+00:00 Mechanisms to Explain the Elemental Composition of the Initial Aragonite Shell of Larval Oysters Haley, Brian A. Hales, Burke Brunner, Elizabeth L. Kovalchik, Kevin Waldbusser, George G. NSF 2018 http://dx.doi.org/10.1002/2017gc007133 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2F2017GC007133 https://agupubs.onlinelibrary.wiley.com/doi/am-pdf/10.1002/2017GC007133 https://agupubs.onlinelibrary.wiley.com/doi/pdf/10.1002/2017GC007133 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#am http://onlinelibrary.wiley.com/termsAndConditions#vor Geochemistry, Geophysics, Geosystems volume 19, issue 4, page 1064-1079 ISSN 1525-2027 1525-2027 journal-article 2018 crwiley https://doi.org/10.1002/2017gc007133 2024-08-13T04:14:00Z Abstract Calcifying organisms face increasing stress from the changing carbonate chemistry of an acidifying ocean, particularly bivalve larvae that live in upwelling regions of the world, such as the coastal and estuarine waters of Oregon (USA). Arguably the first and most significant developmental hurdle faced by larval oysters is formation of their initial prodissoconch I (PDI) shell, upon which further ontological development depends. We measured the minor metal compositions (Sr/Ca, Mg/Ca) of this aragonitic PDI shell and of post‐PDI larval Crassostrea gigas shell, as well as the water they were reared in, over ∼20 days for a May and an August cohort in 2011, during which time there was no period of carbonate under‐saturation. After testing various methods, we successfully isolated the shell from organic tissue using a 5% active chlorine bleach solution. Elemental compositions (Sr, Mg, C, N) of the shells post‐treatment showed that shell Sr/Ca ranged from 1.55 to 1.82 mmol/mol; Mg/Ca from 0.60 to 1.11 mmol/mol, similar to the few comparable published data for larval oyster aragonite compositions. We compare these data in light of possible biomineralization mechanisms: an amorphous calcium carbonate (ACC) path, an intercellular path, and a direct‐from‐seawater path to shell formation via biologically induced inorganic precipitation of aragonite. The last option provides a mechanistic explanation for: (1) the accelerated precipitation rates of biogenic calcification in the absence of a calcifying fluid; (2) consistently elevated precipitation rates at varying ambient‐water saturation states; and (3) the high Ca‐selectivity of the early larval calcification despite rapid precipitation rates. Article in Journal/Newspaper Crassostrea gigas Wiley Online Library Geochemistry, Geophysics, Geosystems 19 4 1064 1079 |
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Wiley Online Library |
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English |
description |
Abstract Calcifying organisms face increasing stress from the changing carbonate chemistry of an acidifying ocean, particularly bivalve larvae that live in upwelling regions of the world, such as the coastal and estuarine waters of Oregon (USA). Arguably the first and most significant developmental hurdle faced by larval oysters is formation of their initial prodissoconch I (PDI) shell, upon which further ontological development depends. We measured the minor metal compositions (Sr/Ca, Mg/Ca) of this aragonitic PDI shell and of post‐PDI larval Crassostrea gigas shell, as well as the water they were reared in, over ∼20 days for a May and an August cohort in 2011, during which time there was no period of carbonate under‐saturation. After testing various methods, we successfully isolated the shell from organic tissue using a 5% active chlorine bleach solution. Elemental compositions (Sr, Mg, C, N) of the shells post‐treatment showed that shell Sr/Ca ranged from 1.55 to 1.82 mmol/mol; Mg/Ca from 0.60 to 1.11 mmol/mol, similar to the few comparable published data for larval oyster aragonite compositions. We compare these data in light of possible biomineralization mechanisms: an amorphous calcium carbonate (ACC) path, an intercellular path, and a direct‐from‐seawater path to shell formation via biologically induced inorganic precipitation of aragonite. The last option provides a mechanistic explanation for: (1) the accelerated precipitation rates of biogenic calcification in the absence of a calcifying fluid; (2) consistently elevated precipitation rates at varying ambient‐water saturation states; and (3) the high Ca‐selectivity of the early larval calcification despite rapid precipitation rates. |
author2 |
NSF |
format |
Article in Journal/Newspaper |
author |
Haley, Brian A. Hales, Burke Brunner, Elizabeth L. Kovalchik, Kevin Waldbusser, George G. |
spellingShingle |
Haley, Brian A. Hales, Burke Brunner, Elizabeth L. Kovalchik, Kevin Waldbusser, George G. Mechanisms to Explain the Elemental Composition of the Initial Aragonite Shell of Larval Oysters |
author_facet |
Haley, Brian A. Hales, Burke Brunner, Elizabeth L. Kovalchik, Kevin Waldbusser, George G. |
author_sort |
Haley, Brian A. |
title |
Mechanisms to Explain the Elemental Composition of the Initial Aragonite Shell of Larval Oysters |
title_short |
Mechanisms to Explain the Elemental Composition of the Initial Aragonite Shell of Larval Oysters |
title_full |
Mechanisms to Explain the Elemental Composition of the Initial Aragonite Shell of Larval Oysters |
title_fullStr |
Mechanisms to Explain the Elemental Composition of the Initial Aragonite Shell of Larval Oysters |
title_full_unstemmed |
Mechanisms to Explain the Elemental Composition of the Initial Aragonite Shell of Larval Oysters |
title_sort |
mechanisms to explain the elemental composition of the initial aragonite shell of larval oysters |
publisher |
Wiley |
publishDate |
2018 |
url |
http://dx.doi.org/10.1002/2017gc007133 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2F2017GC007133 https://agupubs.onlinelibrary.wiley.com/doi/am-pdf/10.1002/2017GC007133 https://agupubs.onlinelibrary.wiley.com/doi/pdf/10.1002/2017GC007133 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Geochemistry, Geophysics, Geosystems volume 19, issue 4, page 1064-1079 ISSN 1525-2027 1525-2027 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#am http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/2017gc007133 |
container_title |
Geochemistry, Geophysics, Geosystems |
container_volume |
19 |
container_issue |
4 |
container_start_page |
1064 |
op_container_end_page |
1079 |
_version_ |
1810440787772571648 |