Potential of temperature- and salinity-driven shifts in diatom compatible solute concentrations to impact biogeochemical cycling within sea ice
Sea-ice algae are an important source of primary production in polar regions, yet we have limited understanding of their responses to the seasonal cycling of temperature and salinity. Using a targeted liquid chromatography-mass spectrometry-based metabolomics approach, we found that axenic cultures...
Published in: | Elementa: Science of the Anthropocene |
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Format: | Article in Journal/Newspaper |
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University of California Press
2020
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Online Access: | http://dx.doi.org/10.1525/elementa.421 http://online.ucpress.edu/elementa/article-pdf/doi/10.1525/elementa.421/441575/421-7239-1-pb.pdf |
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crunicaliforniap:10.1525/elementa.421 2024-09-30T14:25:34+00:00 Potential of temperature- and salinity-driven shifts in diatom compatible solute concentrations to impact biogeochemical cycling within sea ice Dawson, Hannah M. Heal, Katherine R. Boysen, Angela K. Carlson, Laura T. Ingalls, Anitra E. Young, Jodi N. Helmig, Detlev Arrigo, Kevin 2020 http://dx.doi.org/10.1525/elementa.421 http://online.ucpress.edu/elementa/article-pdf/doi/10.1525/elementa.421/441575/421-7239-1-pb.pdf en eng University of California Press http://creativecommons.org/licenses/by/4.0/ Elementa: Science of the Anthropocene volume 8 ISSN 2325-1026 journal-article 2020 crunicaliforniap https://doi.org/10.1525/elementa.421 2024-09-05T05:01:59Z Sea-ice algae are an important source of primary production in polar regions, yet we have limited understanding of their responses to the seasonal cycling of temperature and salinity. Using a targeted liquid chromatography-mass spectrometry-based metabolomics approach, we found that axenic cultures of the Antarctic sea-ice diatom, Nitzschia lecointei, displayed large differences in their metabolomes when grown in a matrix of conditions that included temperatures of –1 and 4°C, and salinities of 32 and 41, despite relatively small changes in growth rate. Temperature exerted a greater effect than salinity on cellular metabolite pool sizes, though the N- or S-containing compatible solutes, 2, 3-dihydroxypropane-1-sulfonate (DHPS), glycine betaine (GBT), dimethylsulfoniopropionate (DMSP), and proline responded strongly to both temperature and salinity, suggesting complexity in their control. We saw the largest (> 4-fold) response to salinity for proline. DHPS, a rarely studied but potential compatible solute, had the highest intracellular concentrations among all compatible solutes of ~85 mM. When comparing the culture findings to natural Arctic sea-ice diatom communities, we found extensive overlap in metabolite profiles, highlighting the relevance of culture-based studies to probe environmental questions. Large changes in sea-ice diatom metabolomes and compatible solutes over a seasonal cycle could be significant components of biogeochemical cycling within sea ice. Article in Journal/Newspaper Antarc* Antarctic Arctic ice algae Sea ice University of California Press Arctic Antarctic The Antarctic Elementa: Science of the Anthropocene 8 |
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Open Polar |
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University of California Press |
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crunicaliforniap |
language |
English |
description |
Sea-ice algae are an important source of primary production in polar regions, yet we have limited understanding of their responses to the seasonal cycling of temperature and salinity. Using a targeted liquid chromatography-mass spectrometry-based metabolomics approach, we found that axenic cultures of the Antarctic sea-ice diatom, Nitzschia lecointei, displayed large differences in their metabolomes when grown in a matrix of conditions that included temperatures of –1 and 4°C, and salinities of 32 and 41, despite relatively small changes in growth rate. Temperature exerted a greater effect than salinity on cellular metabolite pool sizes, though the N- or S-containing compatible solutes, 2, 3-dihydroxypropane-1-sulfonate (DHPS), glycine betaine (GBT), dimethylsulfoniopropionate (DMSP), and proline responded strongly to both temperature and salinity, suggesting complexity in their control. We saw the largest (> 4-fold) response to salinity for proline. DHPS, a rarely studied but potential compatible solute, had the highest intracellular concentrations among all compatible solutes of ~85 mM. When comparing the culture findings to natural Arctic sea-ice diatom communities, we found extensive overlap in metabolite profiles, highlighting the relevance of culture-based studies to probe environmental questions. Large changes in sea-ice diatom metabolomes and compatible solutes over a seasonal cycle could be significant components of biogeochemical cycling within sea ice. |
author2 |
Helmig, Detlev Arrigo, Kevin |
format |
Article in Journal/Newspaper |
author |
Dawson, Hannah M. Heal, Katherine R. Boysen, Angela K. Carlson, Laura T. Ingalls, Anitra E. Young, Jodi N. |
spellingShingle |
Dawson, Hannah M. Heal, Katherine R. Boysen, Angela K. Carlson, Laura T. Ingalls, Anitra E. Young, Jodi N. Potential of temperature- and salinity-driven shifts in diatom compatible solute concentrations to impact biogeochemical cycling within sea ice |
author_facet |
Dawson, Hannah M. Heal, Katherine R. Boysen, Angela K. Carlson, Laura T. Ingalls, Anitra E. Young, Jodi N. |
author_sort |
Dawson, Hannah M. |
title |
Potential of temperature- and salinity-driven shifts in diatom compatible solute concentrations to impact biogeochemical cycling within sea ice |
title_short |
Potential of temperature- and salinity-driven shifts in diatom compatible solute concentrations to impact biogeochemical cycling within sea ice |
title_full |
Potential of temperature- and salinity-driven shifts in diatom compatible solute concentrations to impact biogeochemical cycling within sea ice |
title_fullStr |
Potential of temperature- and salinity-driven shifts in diatom compatible solute concentrations to impact biogeochemical cycling within sea ice |
title_full_unstemmed |
Potential of temperature- and salinity-driven shifts in diatom compatible solute concentrations to impact biogeochemical cycling within sea ice |
title_sort |
potential of temperature- and salinity-driven shifts in diatom compatible solute concentrations to impact biogeochemical cycling within sea ice |
publisher |
University of California Press |
publishDate |
2020 |
url |
http://dx.doi.org/10.1525/elementa.421 http://online.ucpress.edu/elementa/article-pdf/doi/10.1525/elementa.421/441575/421-7239-1-pb.pdf |
geographic |
Arctic Antarctic The Antarctic |
geographic_facet |
Arctic Antarctic The Antarctic |
genre |
Antarc* Antarctic Arctic ice algae Sea ice |
genre_facet |
Antarc* Antarctic Arctic ice algae Sea ice |
op_source |
Elementa: Science of the Anthropocene volume 8 ISSN 2325-1026 |
op_rights |
http://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1525/elementa.421 |
container_title |
Elementa: Science of the Anthropocene |
container_volume |
8 |
_version_ |
1811645563324596224 |