Characterization of Crustacyanin-A2 Subunit as a Component of the Organic Matrix of Gastroliths from the Crayfish Cherax quadricarinatus

• Published in: MRS Proceedings
• Main Authors: Luquet, Gilles, Le Roy, Nathalie, Zanella-Cléon, Isabelle, Becchi, Michel, Bucarey, Sergio, Fernandez, Maria Soledad, Arias, Jose Luis, Guichard, Nathalie, Marie, Benjamin, Marin, Frédéric
• Format: Article in Journal/Newspaper
• Language: English
• Published: Springer Science and Business Media LLC 2009
• Subjects: General Engineering; Homarus gammarus
• Online Access: http://dx.doi.org/10.1557/proc-1187-kk05-03; https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S1946427400012045

Abstract Like the lobsters, some terrestrial crabs and other crayfishes, the Australian red claw crayfish, Cherax quadricarinatus , elaborates in its stomach wall calcium storage structures called gastroliths. For understanding the cyclic elaboration and stabilization of these amorphous calcified structures, we studied the organic matrix (OM) of these paired biomineralizations. After decalcification with acetic acid, we analysed the proteinaceous components of an acetic acid-insoluble fraction by two-dimensional electrophoresis. Nine spots were digested by trpsin and the tryptic peptides were sequenced by nanoLC-nanoESI-MS/MS mass spectrometry. About 100 peptidic sequences were compared to sequences previously registered in the databases. Seven of the partially sequenced organic matrix polypeptides are probably new proteins. Another one corresponds to the previously sequenced protein, GAP65, from Cherax quadricarinatus and the last one, which migrates in electrophoresis at around 25 kDa, presents strong homology with the crustacyanin-A2 subunit of Homarus gammarus .