The infection of reintroduced ruminants – Bison bonasus and Alces alces – with Anaplasma phagocytophilum in northern Poland
Abstract The north-eastern part of Poland is considered an area of high risk for infection with tick-borne diseases, including with human granulocytic ehrlichiosis (HGE) agents. The etiological agent of HGE is Anaplasma phagocytophilum. As the animal reservoir for A. phagocytophilum in the environme...
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crspringernat:10.1515/ap-2015-0091 2023-05-15T13:13:00+02:00 The infection of reintroduced ruminants – Bison bonasus and Alces alces – with Anaplasma phagocytophilum in northern Poland Karbowiak, Grzegorz Víchová, Bronislava Werszko, Joanna Demiaszkiewicz, Aleksander W. Pyziel, Anna M. Sytykiewicz, Hubert Szewczyk, Tomasz Peťko, Branislav 2015 http://dx.doi.org/10.1515/ap-2015-0091 https://www.degruyter.com/view/j/ap.2015.60.issue-4/ap-2015-0091/ap-2015-0091.pdf unknown Springer Science and Business Media LLC Acta Parasitologica volume 60, issue 4 ISSN 1896-1851 1230-2821 Parasitology journal-article 2015 crspringernat https://doi.org/10.1515/ap-2015-0091 2022-01-04T14:53:18Z Abstract The north-eastern part of Poland is considered an area of high risk for infection with tick-borne diseases, including with human granulocytic ehrlichiosis (HGE) agents. The etiological agent of HGE is Anaplasma phagocytophilum. As the animal reservoir for A. phagocytophilum in the environment serve the species from Cervidae and Bovidae families. European bison (Bison bonasus) and elk (Alces alces) are the big ruminant species, reintroduced to the forests of Middle Europe after many decades of absence. In the foci of zoonotic diseases they are able to play a role as natural reservoir to pathogens, however, their status as protected animals means their study has been rare and fragmentary. The studies of B. bonasus were conducted in Białowieża Primeval Forest and A. alces in Biebrza National Park. PCR amplifications were performed using primers amplifing the end of the groES gene, the intergenic spacer and approximately two-thirds of the groEL gene in the first round, and primers that span a 395-bp region of the groEL gene were used in the second round. The positive results were obtained in B. bonasus and A. alces, the prevalence of infection was 66.7 and 20.0%, respectively. Randomly selected samples were sequenced, sequences were compared with GenBank entries using Blast N2.2.13 and determined as A. phagocytophilum. The results presented herein are the first record of the presence of Anaplasma phagocytophilum in A. alces, and at the same time confirm the previous observations regarding the infection of B. bonasus with A. phagocytophilum. Article in Journal/Newspaper Alces alces Springer Nature (via Crossref) Acta Parasitologica 60 4 |
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Parasitology |
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Parasitology Karbowiak, Grzegorz Víchová, Bronislava Werszko, Joanna Demiaszkiewicz, Aleksander W. Pyziel, Anna M. Sytykiewicz, Hubert Szewczyk, Tomasz Peťko, Branislav The infection of reintroduced ruminants – Bison bonasus and Alces alces – with Anaplasma phagocytophilum in northern Poland |
topic_facet |
Parasitology |
description |
Abstract The north-eastern part of Poland is considered an area of high risk for infection with tick-borne diseases, including with human granulocytic ehrlichiosis (HGE) agents. The etiological agent of HGE is Anaplasma phagocytophilum. As the animal reservoir for A. phagocytophilum in the environment serve the species from Cervidae and Bovidae families. European bison (Bison bonasus) and elk (Alces alces) are the big ruminant species, reintroduced to the forests of Middle Europe after many decades of absence. In the foci of zoonotic diseases they are able to play a role as natural reservoir to pathogens, however, their status as protected animals means their study has been rare and fragmentary. The studies of B. bonasus were conducted in Białowieża Primeval Forest and A. alces in Biebrza National Park. PCR amplifications were performed using primers amplifing the end of the groES gene, the intergenic spacer and approximately two-thirds of the groEL gene in the first round, and primers that span a 395-bp region of the groEL gene were used in the second round. The positive results were obtained in B. bonasus and A. alces, the prevalence of infection was 66.7 and 20.0%, respectively. Randomly selected samples were sequenced, sequences were compared with GenBank entries using Blast N2.2.13 and determined as A. phagocytophilum. The results presented herein are the first record of the presence of Anaplasma phagocytophilum in A. alces, and at the same time confirm the previous observations regarding the infection of B. bonasus with A. phagocytophilum. |
format |
Article in Journal/Newspaper |
author |
Karbowiak, Grzegorz Víchová, Bronislava Werszko, Joanna Demiaszkiewicz, Aleksander W. Pyziel, Anna M. Sytykiewicz, Hubert Szewczyk, Tomasz Peťko, Branislav |
author_facet |
Karbowiak, Grzegorz Víchová, Bronislava Werszko, Joanna Demiaszkiewicz, Aleksander W. Pyziel, Anna M. Sytykiewicz, Hubert Szewczyk, Tomasz Peťko, Branislav |
author_sort |
Karbowiak, Grzegorz |
title |
The infection of reintroduced ruminants – Bison bonasus and Alces alces – with Anaplasma phagocytophilum in northern Poland |
title_short |
The infection of reintroduced ruminants – Bison bonasus and Alces alces – with Anaplasma phagocytophilum in northern Poland |
title_full |
The infection of reintroduced ruminants – Bison bonasus and Alces alces – with Anaplasma phagocytophilum in northern Poland |
title_fullStr |
The infection of reintroduced ruminants – Bison bonasus and Alces alces – with Anaplasma phagocytophilum in northern Poland |
title_full_unstemmed |
The infection of reintroduced ruminants – Bison bonasus and Alces alces – with Anaplasma phagocytophilum in northern Poland |
title_sort |
infection of reintroduced ruminants – bison bonasus and alces alces – with anaplasma phagocytophilum in northern poland |
publisher |
Springer Science and Business Media LLC |
publishDate |
2015 |
url |
http://dx.doi.org/10.1515/ap-2015-0091 https://www.degruyter.com/view/j/ap.2015.60.issue-4/ap-2015-0091/ap-2015-0091.pdf |
genre |
Alces alces |
genre_facet |
Alces alces |
op_source |
Acta Parasitologica volume 60, issue 4 ISSN 1896-1851 1230-2821 |
op_doi |
https://doi.org/10.1515/ap-2015-0091 |
container_title |
Acta Parasitologica |
container_volume |
60 |
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4 |
_version_ |
1766255421214949376 |