Roles of three putative salmon louse (Lepeophtheirus salmonis) prostaglandin E2 synthases in physiology and host–parasite interactions
Abstract Background The salmon louse ( Lepeophtheirus salmonis ) is a parasite of salmonid fish. Atlantic salmon ( Salmo salar ) exhibit only a limited and ineffective immune response when infested with this parasite. Prostaglandins (PGs) have many biological functions in both invertebrates and vert...
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crspringernat:10.1186/s13071-021-04690-w 2023-05-15T15:32:51+02:00 Roles of three putative salmon louse (Lepeophtheirus salmonis) prostaglandin E2 synthases in physiology and host–parasite interactions Dalvin, Sussie Eichner, Christiane Dondrup, Michael Øvergård, Aina-Cathrine Research Council Norway 2021 http://dx.doi.org/10.1186/s13071-021-04690-w https://link.springer.com/content/pdf/10.1186/s13071-021-04690-w.pdf https://link.springer.com/article/10.1186/s13071-021-04690-w/fulltext.html en eng Springer Science and Business Media LLC https://creativecommons.org/licenses/by/4.0 https://creativecommons.org/licenses/by/4.0 CC-BY Parasites & Vectors volume 14, issue 1 ISSN 1756-3305 Infectious Diseases Parasitology journal-article 2021 crspringernat https://doi.org/10.1186/s13071-021-04690-w 2022-01-04T13:17:08Z Abstract Background The salmon louse ( Lepeophtheirus salmonis ) is a parasite of salmonid fish. Atlantic salmon ( Salmo salar ) exhibit only a limited and ineffective immune response when infested with this parasite. Prostaglandins (PGs) have many biological functions in both invertebrates and vertebrates, one of which is the regulation of immune responses. This has led to the suggestion that prostaglandin E 2 (PGE 2 ) is important in the salmon louse host–parasite interaction, although studies of a salmon louse prostaglandin E 2 synthase ( PGES ) 2 gene have not enabled conformation of this hypothesis. The aim of the present study was, therefore, to characterize two additional PGES-like genes. Methods Lepeophtheirus salmonis microsomal glutathione S -transferase 1 like ( LsMGST1L ) and LsPGES3L were investigated by sequencing, phylogenetics, transcript localization and expression studies. Moreover, the function of these putative PGES genes in addition to the previously identified LsPGES2 gene was analyzed in double stranded (ds) RNA-mediated knockdown (KD) salmon louse. Results Analysis of the three putative LsPGES genes showed a rather constitutive transcript level throughout development from nauplius to the adult stages, and in a range of tissues, with the highest levels in the ovaries or gut. DsRNA-mediated KD of these transcripts did not produce any characteristic changes in phenotype, and KD animals displayed a normal reproductive output. The ability of the parasite to infect or modulate the immune response of the host fish was also not affected by KD. Conclusions Salmon louse prostaglandins may play endogenous roles in the management of reproduction and oxidative stress and may be a product of salmon louse blood digestions. Graphical Abstract Article in Journal/Newspaper Atlantic salmon Salmo salar Springer Nature (via Crossref) Parasites & Vectors 14 1 |
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Springer Nature (via Crossref) |
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English |
topic |
Infectious Diseases Parasitology |
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Infectious Diseases Parasitology Dalvin, Sussie Eichner, Christiane Dondrup, Michael Øvergård, Aina-Cathrine Roles of three putative salmon louse (Lepeophtheirus salmonis) prostaglandin E2 synthases in physiology and host–parasite interactions |
topic_facet |
Infectious Diseases Parasitology |
description |
Abstract Background The salmon louse ( Lepeophtheirus salmonis ) is a parasite of salmonid fish. Atlantic salmon ( Salmo salar ) exhibit only a limited and ineffective immune response when infested with this parasite. Prostaglandins (PGs) have many biological functions in both invertebrates and vertebrates, one of which is the regulation of immune responses. This has led to the suggestion that prostaglandin E 2 (PGE 2 ) is important in the salmon louse host–parasite interaction, although studies of a salmon louse prostaglandin E 2 synthase ( PGES ) 2 gene have not enabled conformation of this hypothesis. The aim of the present study was, therefore, to characterize two additional PGES-like genes. Methods Lepeophtheirus salmonis microsomal glutathione S -transferase 1 like ( LsMGST1L ) and LsPGES3L were investigated by sequencing, phylogenetics, transcript localization and expression studies. Moreover, the function of these putative PGES genes in addition to the previously identified LsPGES2 gene was analyzed in double stranded (ds) RNA-mediated knockdown (KD) salmon louse. Results Analysis of the three putative LsPGES genes showed a rather constitutive transcript level throughout development from nauplius to the adult stages, and in a range of tissues, with the highest levels in the ovaries or gut. DsRNA-mediated KD of these transcripts did not produce any characteristic changes in phenotype, and KD animals displayed a normal reproductive output. The ability of the parasite to infect or modulate the immune response of the host fish was also not affected by KD. Conclusions Salmon louse prostaglandins may play endogenous roles in the management of reproduction and oxidative stress and may be a product of salmon louse blood digestions. Graphical Abstract |
author2 |
Research Council Norway |
format |
Article in Journal/Newspaper |
author |
Dalvin, Sussie Eichner, Christiane Dondrup, Michael Øvergård, Aina-Cathrine |
author_facet |
Dalvin, Sussie Eichner, Christiane Dondrup, Michael Øvergård, Aina-Cathrine |
author_sort |
Dalvin, Sussie |
title |
Roles of three putative salmon louse (Lepeophtheirus salmonis) prostaglandin E2 synthases in physiology and host–parasite interactions |
title_short |
Roles of three putative salmon louse (Lepeophtheirus salmonis) prostaglandin E2 synthases in physiology and host–parasite interactions |
title_full |
Roles of three putative salmon louse (Lepeophtheirus salmonis) prostaglandin E2 synthases in physiology and host–parasite interactions |
title_fullStr |
Roles of three putative salmon louse (Lepeophtheirus salmonis) prostaglandin E2 synthases in physiology and host–parasite interactions |
title_full_unstemmed |
Roles of three putative salmon louse (Lepeophtheirus salmonis) prostaglandin E2 synthases in physiology and host–parasite interactions |
title_sort |
roles of three putative salmon louse (lepeophtheirus salmonis) prostaglandin e2 synthases in physiology and host–parasite interactions |
publisher |
Springer Science and Business Media LLC |
publishDate |
2021 |
url |
http://dx.doi.org/10.1186/s13071-021-04690-w https://link.springer.com/content/pdf/10.1186/s13071-021-04690-w.pdf https://link.springer.com/article/10.1186/s13071-021-04690-w/fulltext.html |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Parasites & Vectors volume 14, issue 1 ISSN 1756-3305 |
op_rights |
https://creativecommons.org/licenses/by/4.0 https://creativecommons.org/licenses/by/4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1186/s13071-021-04690-w |
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14 |
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