Rickettsia spp. in bats of Romania: high prevalence of Rickettsia monacensis in two insectivorous bat species
Abstract Background Spotted fever group rickettsiae represent one of the most diverse groups of vector-borne bacteria, with several human pathogenic species showing an emerging trend worldwide. Most species are vectored by ticks (Ixodidae), with many zoonotic reservoir species among most terrestrial...
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crspringernat:10.1186/s13071-021-04592-x 2023-05-15T17:48:39+02:00 Rickettsia spp. in bats of Romania: high prevalence of Rickettsia monacensis in two insectivorous bat species Matei, Ioana A. Corduneanu, Alexandra Sándor, Attila D. Ionică, Angela Monica Panait, Luciana Kalmár, Zsuzsa Ivan, Talida Papuc, Ionel Bouari, Cosmina Fit, Nicodim Mihalca, Andrei Daniel CNCS-UEFISCDI János Bolyai Research Scholarship of Hungarian Academy of Science CNCS-UEFISCDI 2021 http://dx.doi.org/10.1186/s13071-021-04592-x https://link.springer.com/content/pdf/10.1186/s13071-021-04592-x.pdf https://link.springer.com/article/10.1186/s13071-021-04592-x/fulltext.html en eng Springer Science and Business Media LLC https://creativecommons.org/licenses/by/4.0 https://creativecommons.org/licenses/by/4.0 CC-BY Parasites & Vectors volume 14, issue 1 ISSN 1756-3305 Infectious Diseases Parasitology journal-article 2021 crspringernat https://doi.org/10.1186/s13071-021-04592-x 2022-01-04T16:10:57Z Abstract Background Spotted fever group rickettsiae represent one of the most diverse groups of vector-borne bacteria, with several human pathogenic species showing an emerging trend worldwide. Most species are vectored by ticks (Ixodidae), with many zoonotic reservoir species among most terrestrial vertebrate groups. While the reservoir competence of many different vertebrate species is well known (e.g. birds, rodents and dogs), studies on insectivorous bats have been rarely performed despite their high species diversity, ubiquitous urban presence and importance in harboring zoonotic disease agents. Romania has a high diversity and ubiquity of bats. Moreover, seven out of eight SFG rickettsiae species with zoonotic potential were previously reported in Romania. Based on this, the aim of this study was to detect Rickettsia species in tissue samples in bats. Methods Here we report a large-scale study (322 bats belonging to 20 species) on the presence of Rickettsia spp. in Romanian bat species. Tissue samples from insectivorous bats were tested for the presence of Rickettsia DNA using PCR detection amplifying a 381 bp fragment of the gltA gene. Positive results were sequenced to confirm the results. The obtained results were statistically analyzed by chi-squared independence test. Results Positive results were obtained in 14.6% of bat samples. Sequence analysis confirmed the presence of R. monacensis in two bat species ( Nyctalus noctula and Pipistrellus pipistrellus ) in two locations. Conclusion This study provides the first evidence of a possible involvement of these bat species in the epidemiology of Rickettsia spp., highlighting the importance of bats in natural cycles of these vector-borne pathogens. Graphical Abstract Article in Journal/Newspaper Nyctalus noctula Pipistrellus pipistrellus Springer Nature (via Crossref) Parasites & Vectors 14 1 |
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Springer Nature (via Crossref) |
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English |
topic |
Infectious Diseases Parasitology |
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Infectious Diseases Parasitology Matei, Ioana A. Corduneanu, Alexandra Sándor, Attila D. Ionică, Angela Monica Panait, Luciana Kalmár, Zsuzsa Ivan, Talida Papuc, Ionel Bouari, Cosmina Fit, Nicodim Mihalca, Andrei Daniel Rickettsia spp. in bats of Romania: high prevalence of Rickettsia monacensis in two insectivorous bat species |
topic_facet |
Infectious Diseases Parasitology |
description |
Abstract Background Spotted fever group rickettsiae represent one of the most diverse groups of vector-borne bacteria, with several human pathogenic species showing an emerging trend worldwide. Most species are vectored by ticks (Ixodidae), with many zoonotic reservoir species among most terrestrial vertebrate groups. While the reservoir competence of many different vertebrate species is well known (e.g. birds, rodents and dogs), studies on insectivorous bats have been rarely performed despite their high species diversity, ubiquitous urban presence and importance in harboring zoonotic disease agents. Romania has a high diversity and ubiquity of bats. Moreover, seven out of eight SFG rickettsiae species with zoonotic potential were previously reported in Romania. Based on this, the aim of this study was to detect Rickettsia species in tissue samples in bats. Methods Here we report a large-scale study (322 bats belonging to 20 species) on the presence of Rickettsia spp. in Romanian bat species. Tissue samples from insectivorous bats were tested for the presence of Rickettsia DNA using PCR detection amplifying a 381 bp fragment of the gltA gene. Positive results were sequenced to confirm the results. The obtained results were statistically analyzed by chi-squared independence test. Results Positive results were obtained in 14.6% of bat samples. Sequence analysis confirmed the presence of R. monacensis in two bat species ( Nyctalus noctula and Pipistrellus pipistrellus ) in two locations. Conclusion This study provides the first evidence of a possible involvement of these bat species in the epidemiology of Rickettsia spp., highlighting the importance of bats in natural cycles of these vector-borne pathogens. Graphical Abstract |
author2 |
CNCS-UEFISCDI János Bolyai Research Scholarship of Hungarian Academy of Science CNCS-UEFISCDI |
format |
Article in Journal/Newspaper |
author |
Matei, Ioana A. Corduneanu, Alexandra Sándor, Attila D. Ionică, Angela Monica Panait, Luciana Kalmár, Zsuzsa Ivan, Talida Papuc, Ionel Bouari, Cosmina Fit, Nicodim Mihalca, Andrei Daniel |
author_facet |
Matei, Ioana A. Corduneanu, Alexandra Sándor, Attila D. Ionică, Angela Monica Panait, Luciana Kalmár, Zsuzsa Ivan, Talida Papuc, Ionel Bouari, Cosmina Fit, Nicodim Mihalca, Andrei Daniel |
author_sort |
Matei, Ioana A. |
title |
Rickettsia spp. in bats of Romania: high prevalence of Rickettsia monacensis in two insectivorous bat species |
title_short |
Rickettsia spp. in bats of Romania: high prevalence of Rickettsia monacensis in two insectivorous bat species |
title_full |
Rickettsia spp. in bats of Romania: high prevalence of Rickettsia monacensis in two insectivorous bat species |
title_fullStr |
Rickettsia spp. in bats of Romania: high prevalence of Rickettsia monacensis in two insectivorous bat species |
title_full_unstemmed |
Rickettsia spp. in bats of Romania: high prevalence of Rickettsia monacensis in two insectivorous bat species |
title_sort |
rickettsia spp. in bats of romania: high prevalence of rickettsia monacensis in two insectivorous bat species |
publisher |
Springer Science and Business Media LLC |
publishDate |
2021 |
url |
http://dx.doi.org/10.1186/s13071-021-04592-x https://link.springer.com/content/pdf/10.1186/s13071-021-04592-x.pdf https://link.springer.com/article/10.1186/s13071-021-04592-x/fulltext.html |
genre |
Nyctalus noctula Pipistrellus pipistrellus |
genre_facet |
Nyctalus noctula Pipistrellus pipistrellus |
op_source |
Parasites & Vectors volume 14, issue 1 ISSN 1756-3305 |
op_rights |
https://creativecommons.org/licenses/by/4.0 https://creativecommons.org/licenses/by/4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1186/s13071-021-04592-x |
container_title |
Parasites & Vectors |
container_volume |
14 |
container_issue |
1 |
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1766154781026418688 |