First detection of Enterocytozoon bieneusi in whooper swans (Cygnus cygnus) in China
Abstract Background Enterocytozoon bieneusi is a parasite that infects humans and a wide range of other animals. The large migratory waterfowl, the whooper swan ( Cygnus cygnus ), travels through many cities during its migration and can spread parasites. Despite receiving increasing attention worldw...
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crspringernat:10.1186/s13071-020-3884-y 2023-05-15T15:59:48+02:00 First detection of Enterocytozoon bieneusi in whooper swans (Cygnus cygnus) in China Wang, Yuexin Zhang, Kaihui Zhang, Yifan Wang, Ke Gazizova, Azhar Wang, Luyang Cao, Letian Zhang, Yajun Huang, Jianying Cui, Yuan Zhang, Yuxi Zhang, Longxian Natural Science Foundation of Henan Province National Key Research and Development Program of China National Natural Science Foundation of China 2020 http://dx.doi.org/10.1186/s13071-020-3884-y http://link.springer.com/content/pdf/10.1186/s13071-020-3884-y.pdf http://link.springer.com/article/10.1186/s13071-020-3884-y/fulltext.html en eng Springer Science and Business Media LLC http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/ CC-BY Parasites & Vectors volume 13, issue 1 ISSN 1756-3305 Infectious Diseases Parasitology journal-article 2020 crspringernat https://doi.org/10.1186/s13071-020-3884-y 2022-01-04T10:35:09Z Abstract Background Enterocytozoon bieneusi is a parasite that infects humans and a wide range of other animals. The large migratory waterfowl, the whooper swan ( Cygnus cygnus ), travels through many cities during its migration and can spread parasites. Despite receiving increasing attention worldwide, there have been no reports of E. bieneusi infection occurring in C. cygnus . Therefore, this study aims to assess the prevalence and genetic characteristics of E. bieneusi in C. cygnus in Sanmenxia, China. Methods Altogether, 467 fresh fecal samples were collected in the Swan Wetland Park in Sanmenxia, China. Genomic DNA was extracted from fresh fecal samples ( n = 467) and E. bieneusi was identified by nested PCR amplification of the internal transcribed spacer (ITS) region. ITS-positive sequences were aligned and phylogenetically analyzed to determine the genotypes of E. bieneusi . Results The overall prevalence of E. bieneusi in C. cygnus was 7.49% (35/467). Sequencing of the 35 positive samples revealed eight known genotypes (EbpA, EbpC, Henan-III, Henan-IV, BEB6, CD9, Peru6 and PtEb IX) and three novel genotypes (CSW1, CSW2 and CSW3). The phylogenetic tree constructed from the ITS sequences showed that seven genotypes (Peru6, EbpA, EbpC, Henan-III, CSW3, Henan-IV and CSW1) clustered within the zoonotic Group 1 while the remaining novel genotype CSW2 clustered within Group 5. Conclusions To our knowledge, this is the first report of E. bieneusi in C. cygnus . Of public health significance, our results suggest that migratory C. cygnus might play an important role in the water-borne transmission of E. bieneusi . Effective strategies will be necessary to control E. bieneusi infection in C. cygnus , other animals and humans. Article in Journal/Newspaper Cygnus cygnus Whooper Swan Springer Nature (via Crossref) Parasites & Vectors 13 1 |
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Open Polar |
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Springer Nature (via Crossref) |
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English |
topic |
Infectious Diseases Parasitology |
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Infectious Diseases Parasitology Wang, Yuexin Zhang, Kaihui Zhang, Yifan Wang, Ke Gazizova, Azhar Wang, Luyang Cao, Letian Zhang, Yajun Huang, Jianying Cui, Yuan Zhang, Yuxi Zhang, Longxian First detection of Enterocytozoon bieneusi in whooper swans (Cygnus cygnus) in China |
topic_facet |
Infectious Diseases Parasitology |
description |
Abstract Background Enterocytozoon bieneusi is a parasite that infects humans and a wide range of other animals. The large migratory waterfowl, the whooper swan ( Cygnus cygnus ), travels through many cities during its migration and can spread parasites. Despite receiving increasing attention worldwide, there have been no reports of E. bieneusi infection occurring in C. cygnus . Therefore, this study aims to assess the prevalence and genetic characteristics of E. bieneusi in C. cygnus in Sanmenxia, China. Methods Altogether, 467 fresh fecal samples were collected in the Swan Wetland Park in Sanmenxia, China. Genomic DNA was extracted from fresh fecal samples ( n = 467) and E. bieneusi was identified by nested PCR amplification of the internal transcribed spacer (ITS) region. ITS-positive sequences were aligned and phylogenetically analyzed to determine the genotypes of E. bieneusi . Results The overall prevalence of E. bieneusi in C. cygnus was 7.49% (35/467). Sequencing of the 35 positive samples revealed eight known genotypes (EbpA, EbpC, Henan-III, Henan-IV, BEB6, CD9, Peru6 and PtEb IX) and three novel genotypes (CSW1, CSW2 and CSW3). The phylogenetic tree constructed from the ITS sequences showed that seven genotypes (Peru6, EbpA, EbpC, Henan-III, CSW3, Henan-IV and CSW1) clustered within the zoonotic Group 1 while the remaining novel genotype CSW2 clustered within Group 5. Conclusions To our knowledge, this is the first report of E. bieneusi in C. cygnus . Of public health significance, our results suggest that migratory C. cygnus might play an important role in the water-borne transmission of E. bieneusi . Effective strategies will be necessary to control E. bieneusi infection in C. cygnus , other animals and humans. |
author2 |
Natural Science Foundation of Henan Province National Key Research and Development Program of China National Natural Science Foundation of China |
format |
Article in Journal/Newspaper |
author |
Wang, Yuexin Zhang, Kaihui Zhang, Yifan Wang, Ke Gazizova, Azhar Wang, Luyang Cao, Letian Zhang, Yajun Huang, Jianying Cui, Yuan Zhang, Yuxi Zhang, Longxian |
author_facet |
Wang, Yuexin Zhang, Kaihui Zhang, Yifan Wang, Ke Gazizova, Azhar Wang, Luyang Cao, Letian Zhang, Yajun Huang, Jianying Cui, Yuan Zhang, Yuxi Zhang, Longxian |
author_sort |
Wang, Yuexin |
title |
First detection of Enterocytozoon bieneusi in whooper swans (Cygnus cygnus) in China |
title_short |
First detection of Enterocytozoon bieneusi in whooper swans (Cygnus cygnus) in China |
title_full |
First detection of Enterocytozoon bieneusi in whooper swans (Cygnus cygnus) in China |
title_fullStr |
First detection of Enterocytozoon bieneusi in whooper swans (Cygnus cygnus) in China |
title_full_unstemmed |
First detection of Enterocytozoon bieneusi in whooper swans (Cygnus cygnus) in China |
title_sort |
first detection of enterocytozoon bieneusi in whooper swans (cygnus cygnus) in china |
publisher |
Springer Science and Business Media LLC |
publishDate |
2020 |
url |
http://dx.doi.org/10.1186/s13071-020-3884-y http://link.springer.com/content/pdf/10.1186/s13071-020-3884-y.pdf http://link.springer.com/article/10.1186/s13071-020-3884-y/fulltext.html |
genre |
Cygnus cygnus Whooper Swan |
genre_facet |
Cygnus cygnus Whooper Swan |
op_source |
Parasites & Vectors volume 13, issue 1 ISSN 1756-3305 |
op_rights |
http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/ |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1186/s13071-020-3884-y |
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Parasites & Vectors |
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