Molecular characterization of ascaridoid parasites from captive wild carnivores in China using ribosomal and mitochondrial sequences
Abstract Background Despite the public health importance of toxocariasis/toxascariasis, only a few species of these ascaridoid parasites from wild canine and feline carnivores have been studied at the molecular level so far. Poor understanding of diversity, host distribution and the potential (zoono...
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Online Access: | http://dx.doi.org/10.1186/s13071-020-04254-4 https://link.springer.com/content/pdf/10.1186/s13071-020-04254-4.pdf https://link.springer.com/article/10.1186/s13071-020-04254-4/fulltext.html |
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crspringernat:10.1186/s13071-020-04254-4 2023-05-15T13:19:53+02:00 Molecular characterization of ascaridoid parasites from captive wild carnivores in China using ribosomal and mitochondrial sequences Xie, Yue Li, Yingxin Gu, Xiaobin Liu, Yunjian Zhou, Xuan Wang, Lu He, Ran Peng, Xuerong Yang, Guangyou Sichuan International Science and Technology Innovation Cooperation/Hong Kong, Macao and Taiwan Science and Technology Innovation Cooperation Project Key Open Laboratory of Conservation Biology of Rare Animals in Giant Panda National Park, State Forestry and Grassland Administration High-level Scientific Research Foundation for the Introduction of Talents of Sichuan Agricultural University 2020 http://dx.doi.org/10.1186/s13071-020-04254-4 https://link.springer.com/content/pdf/10.1186/s13071-020-04254-4.pdf https://link.springer.com/article/10.1186/s13071-020-04254-4/fulltext.html en eng Springer Science and Business Media LLC https://creativecommons.org/licenses/by/4.0 https://creativecommons.org/licenses/by/4.0 CC-BY Parasites & Vectors volume 13, issue 1 ISSN 1756-3305 Infectious Diseases Parasitology journal-article 2020 crspringernat https://doi.org/10.1186/s13071-020-04254-4 2022-01-04T16:50:12Z Abstract Background Despite the public health importance of toxocariasis/toxascariasis, only a few species of these ascaridoid parasites from wild canine and feline carnivores have been studied at the molecular level so far. Poor understanding of diversity, host distribution and the potential (zoonotic) transmission of the ascaridoid species among wild animals negatively affects their surveillance and control in natural settings. In this study, we updated previous knowledge by profiling the genetic diversity and phylogenetic relationships of ascaridoid species among eleven wild canine and feline animals on the basis of a combined analysis of the ribosomal internal transcribed spacer region (ITS) gene and the partial mitochondrial cytochrome c oxidase subunit 2 ( cox 2) and NADH dehydrogenase subunit 1 ( nad 1) genes. Results In total, three genetically distinct ascaridoid lineages were determined to be present among these wild carnivores sampled, including Toxocara canis in Alopex lagopus and Vulpes vulpes , Toxocara cati in Felis chaus , Prionailurus bengalensis and Catopuma temmincki and Toxascaris leonina in Canis lupus , Panthera tigris altaica , Panthera tigris amoyensis , Panthera tigris tigris , Panthera leo and Lynx lynx . Furthermore, it was evident that T. leonina lineage split into three well-supported subclades depending on their host species, i.e. wild felids, dogs and wolves and foxes, based on integrated genetic and phylogenetic evidence, supporting that a complex of T. leonina other than one species infecting these hosts. Conclusions These results provide new molecular insights into classification, phylogenetic relationships and epidemiological importance of ascaridoids from wild canids and felids and also highlight the complex of the taxonomy and genetics of Toxascaris in their wild and domestic carnivorous hosts. Article in Journal/Newspaper Alopex lagopus Canis lupus Lynx Springer Nature (via Crossref) Parasites & Vectors 13 1 |
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Springer Nature (via Crossref) |
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English |
topic |
Infectious Diseases Parasitology |
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Infectious Diseases Parasitology Xie, Yue Li, Yingxin Gu, Xiaobin Liu, Yunjian Zhou, Xuan Wang, Lu He, Ran Peng, Xuerong Yang, Guangyou Molecular characterization of ascaridoid parasites from captive wild carnivores in China using ribosomal and mitochondrial sequences |
topic_facet |
Infectious Diseases Parasitology |
description |
Abstract Background Despite the public health importance of toxocariasis/toxascariasis, only a few species of these ascaridoid parasites from wild canine and feline carnivores have been studied at the molecular level so far. Poor understanding of diversity, host distribution and the potential (zoonotic) transmission of the ascaridoid species among wild animals negatively affects their surveillance and control in natural settings. In this study, we updated previous knowledge by profiling the genetic diversity and phylogenetic relationships of ascaridoid species among eleven wild canine and feline animals on the basis of a combined analysis of the ribosomal internal transcribed spacer region (ITS) gene and the partial mitochondrial cytochrome c oxidase subunit 2 ( cox 2) and NADH dehydrogenase subunit 1 ( nad 1) genes. Results In total, three genetically distinct ascaridoid lineages were determined to be present among these wild carnivores sampled, including Toxocara canis in Alopex lagopus and Vulpes vulpes , Toxocara cati in Felis chaus , Prionailurus bengalensis and Catopuma temmincki and Toxascaris leonina in Canis lupus , Panthera tigris altaica , Panthera tigris amoyensis , Panthera tigris tigris , Panthera leo and Lynx lynx . Furthermore, it was evident that T. leonina lineage split into three well-supported subclades depending on their host species, i.e. wild felids, dogs and wolves and foxes, based on integrated genetic and phylogenetic evidence, supporting that a complex of T. leonina other than one species infecting these hosts. Conclusions These results provide new molecular insights into classification, phylogenetic relationships and epidemiological importance of ascaridoids from wild canids and felids and also highlight the complex of the taxonomy and genetics of Toxascaris in their wild and domestic carnivorous hosts. |
author2 |
Sichuan International Science and Technology Innovation Cooperation/Hong Kong, Macao and Taiwan Science and Technology Innovation Cooperation Project Key Open Laboratory of Conservation Biology of Rare Animals in Giant Panda National Park, State Forestry and Grassland Administration High-level Scientific Research Foundation for the Introduction of Talents of Sichuan Agricultural University |
format |
Article in Journal/Newspaper |
author |
Xie, Yue Li, Yingxin Gu, Xiaobin Liu, Yunjian Zhou, Xuan Wang, Lu He, Ran Peng, Xuerong Yang, Guangyou |
author_facet |
Xie, Yue Li, Yingxin Gu, Xiaobin Liu, Yunjian Zhou, Xuan Wang, Lu He, Ran Peng, Xuerong Yang, Guangyou |
author_sort |
Xie, Yue |
title |
Molecular characterization of ascaridoid parasites from captive wild carnivores in China using ribosomal and mitochondrial sequences |
title_short |
Molecular characterization of ascaridoid parasites from captive wild carnivores in China using ribosomal and mitochondrial sequences |
title_full |
Molecular characterization of ascaridoid parasites from captive wild carnivores in China using ribosomal and mitochondrial sequences |
title_fullStr |
Molecular characterization of ascaridoid parasites from captive wild carnivores in China using ribosomal and mitochondrial sequences |
title_full_unstemmed |
Molecular characterization of ascaridoid parasites from captive wild carnivores in China using ribosomal and mitochondrial sequences |
title_sort |
molecular characterization of ascaridoid parasites from captive wild carnivores in china using ribosomal and mitochondrial sequences |
publisher |
Springer Science and Business Media LLC |
publishDate |
2020 |
url |
http://dx.doi.org/10.1186/s13071-020-04254-4 https://link.springer.com/content/pdf/10.1186/s13071-020-04254-4.pdf https://link.springer.com/article/10.1186/s13071-020-04254-4/fulltext.html |
genre |
Alopex lagopus Canis lupus Lynx |
genre_facet |
Alopex lagopus Canis lupus Lynx |
op_source |
Parasites & Vectors volume 13, issue 1 ISSN 1756-3305 |
op_rights |
https://creativecommons.org/licenses/by/4.0 https://creativecommons.org/licenses/by/4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1186/s13071-020-04254-4 |
container_title |
Parasites & Vectors |
container_volume |
13 |
container_issue |
1 |
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1766350118751043584 |