Echinococcus multilocularis and Echinococcus granulosus in canines in North-Khorasan Province, northeastern Iran, identified using morphology and genetic characterization of mitochondrial DNA
Abstract Background Canids are definitive hosts of Echinococcus multilocularis and Echinococcus granulosus. This study aimed to survey these two Echinococcus species in canids of North-Khorasan Province, northeastern Iran, using morphological criteria and genetic characterization of mitochondrial DN...
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2019
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Online Access: | http://dx.doi.org/10.1186/s13071-019-3859-z http://link.springer.com/content/pdf/10.1186/s13071-019-3859-z.pdf http://link.springer.com/article/10.1186/s13071-019-3859-z/fulltext.html |
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crspringernat:10.1186/s13071-019-3859-z 2023-05-15T15:51:03+02:00 Echinococcus multilocularis and Echinococcus granulosus in canines in North-Khorasan Province, northeastern Iran, identified using morphology and genetic characterization of mitochondrial DNA Heidari, Zahra Sharbatkhori, Mitra Mobedi, Iraj Mirhendi, Seyed Hossein Nikmanesh, Bahram Sharifdini, Meysam Mohebali, Mehdi Zarei, Zabihollah Arzamani, Kourosh Kia, Eshrat Beigom Tehran University of Medical Sciences 2019 http://dx.doi.org/10.1186/s13071-019-3859-z http://link.springer.com/content/pdf/10.1186/s13071-019-3859-z.pdf http://link.springer.com/article/10.1186/s13071-019-3859-z/fulltext.html en eng Springer Science and Business Media LLC http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/ CC-BY Parasites & Vectors volume 12, issue 1 ISSN 1756-3305 Infectious Diseases Parasitology journal-article 2019 crspringernat https://doi.org/10.1186/s13071-019-3859-z 2022-01-04T14:12:36Z Abstract Background Canids are definitive hosts of Echinococcus multilocularis and Echinococcus granulosus. This study aimed to survey these two Echinococcus species in canids of North-Khorasan Province, northeastern Iran, using morphological criteria and genetic characterization of mitochondrial DNA. Methods The carcasses of 106 canids, namely 61 jackals ( Canis aureus ), 23 foxes ( Vulpes vulpes ), 19 dogs ( Canis familiaris ) and three wolves ( Canis lupus ) were collected from the study area in 2013–2014 and examined for Echinococcus species. Morphological features were assessed by microscopy of adult worms. For molecular characterization, DNA was extracted, mostly from the adult worms but also from eggs. DNA fragments of the cytochrome c oxidase subunit 1 ( cox 1) and NADH dehydrogenase subunit 1 ( nad 1) mitochondrial genes were amplified and sequenced. Sequences were aligned and compared with reference sequences. Intraspecific and interspecific diversity were calculated and phylogenetic analysis was performed. Results Overall, 9.4% of the canids (eight jackals and two foxes) were found infected with E. multilocularis by molecular methods, of which seven cases were also confirmed using morphological description of the adult worms. Echinococcus granulosus was found in 6.6% of the canines (four dogs, two jackals and one wolf) as determined by both molecular methods and adult cestode morphology. All E. granulosus isolates were identified as the G1 genotype. Comparative sequence analysis indicated 0–0.7% and 0% intraspecific divergence within E. granulosus isolates and 0% and 0–0.2% within E. multilocularis isolates for cox 1 and nad 1, respectively. Conclusions This study revealed the presence of E. multilocularis and E. granulosus in canids of North-Khorasan Province of Iran. Jackals were found infected with both E. multilocularis and E. granulosus , but infection with the former species was higher. Article in Journal/Newspaper Canis lupus Springer Nature (via Crossref) Parasites & Vectors 12 1 |
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Springer Nature (via Crossref) |
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English |
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Infectious Diseases Parasitology |
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Infectious Diseases Parasitology Heidari, Zahra Sharbatkhori, Mitra Mobedi, Iraj Mirhendi, Seyed Hossein Nikmanesh, Bahram Sharifdini, Meysam Mohebali, Mehdi Zarei, Zabihollah Arzamani, Kourosh Kia, Eshrat Beigom Echinococcus multilocularis and Echinococcus granulosus in canines in North-Khorasan Province, northeastern Iran, identified using morphology and genetic characterization of mitochondrial DNA |
topic_facet |
Infectious Diseases Parasitology |
description |
Abstract Background Canids are definitive hosts of Echinococcus multilocularis and Echinococcus granulosus. This study aimed to survey these two Echinococcus species in canids of North-Khorasan Province, northeastern Iran, using morphological criteria and genetic characterization of mitochondrial DNA. Methods The carcasses of 106 canids, namely 61 jackals ( Canis aureus ), 23 foxes ( Vulpes vulpes ), 19 dogs ( Canis familiaris ) and three wolves ( Canis lupus ) were collected from the study area in 2013–2014 and examined for Echinococcus species. Morphological features were assessed by microscopy of adult worms. For molecular characterization, DNA was extracted, mostly from the adult worms but also from eggs. DNA fragments of the cytochrome c oxidase subunit 1 ( cox 1) and NADH dehydrogenase subunit 1 ( nad 1) mitochondrial genes were amplified and sequenced. Sequences were aligned and compared with reference sequences. Intraspecific and interspecific diversity were calculated and phylogenetic analysis was performed. Results Overall, 9.4% of the canids (eight jackals and two foxes) were found infected with E. multilocularis by molecular methods, of which seven cases were also confirmed using morphological description of the adult worms. Echinococcus granulosus was found in 6.6% of the canines (four dogs, two jackals and one wolf) as determined by both molecular methods and adult cestode morphology. All E. granulosus isolates were identified as the G1 genotype. Comparative sequence analysis indicated 0–0.7% and 0% intraspecific divergence within E. granulosus isolates and 0% and 0–0.2% within E. multilocularis isolates for cox 1 and nad 1, respectively. Conclusions This study revealed the presence of E. multilocularis and E. granulosus in canids of North-Khorasan Province of Iran. Jackals were found infected with both E. multilocularis and E. granulosus , but infection with the former species was higher. |
author2 |
Tehran University of Medical Sciences |
format |
Article in Journal/Newspaper |
author |
Heidari, Zahra Sharbatkhori, Mitra Mobedi, Iraj Mirhendi, Seyed Hossein Nikmanesh, Bahram Sharifdini, Meysam Mohebali, Mehdi Zarei, Zabihollah Arzamani, Kourosh Kia, Eshrat Beigom |
author_facet |
Heidari, Zahra Sharbatkhori, Mitra Mobedi, Iraj Mirhendi, Seyed Hossein Nikmanesh, Bahram Sharifdini, Meysam Mohebali, Mehdi Zarei, Zabihollah Arzamani, Kourosh Kia, Eshrat Beigom |
author_sort |
Heidari, Zahra |
title |
Echinococcus multilocularis and Echinococcus granulosus in canines in North-Khorasan Province, northeastern Iran, identified using morphology and genetic characterization of mitochondrial DNA |
title_short |
Echinococcus multilocularis and Echinococcus granulosus in canines in North-Khorasan Province, northeastern Iran, identified using morphology and genetic characterization of mitochondrial DNA |
title_full |
Echinococcus multilocularis and Echinococcus granulosus in canines in North-Khorasan Province, northeastern Iran, identified using morphology and genetic characterization of mitochondrial DNA |
title_fullStr |
Echinococcus multilocularis and Echinococcus granulosus in canines in North-Khorasan Province, northeastern Iran, identified using morphology and genetic characterization of mitochondrial DNA |
title_full_unstemmed |
Echinococcus multilocularis and Echinococcus granulosus in canines in North-Khorasan Province, northeastern Iran, identified using morphology and genetic characterization of mitochondrial DNA |
title_sort |
echinococcus multilocularis and echinococcus granulosus in canines in north-khorasan province, northeastern iran, identified using morphology and genetic characterization of mitochondrial dna |
publisher |
Springer Science and Business Media LLC |
publishDate |
2019 |
url |
http://dx.doi.org/10.1186/s13071-019-3859-z http://link.springer.com/content/pdf/10.1186/s13071-019-3859-z.pdf http://link.springer.com/article/10.1186/s13071-019-3859-z/fulltext.html |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_source |
Parasites & Vectors volume 12, issue 1 ISSN 1756-3305 |
op_rights |
http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/ |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1186/s13071-019-3859-z |
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Parasites & Vectors |
container_volume |
12 |
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1 |
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1766386112753827840 |