Choline supplementation prevents diet induced gut mucosa lipid accumulation in post-smolt Atlantic salmon (Salmo salar L.)
Abstract Background Various intestinal morphological alterations have been reported in cultured fish fed diets with high contents of plant ingredients. Since 2000, salmon farmers have reported symptoms indicating an intestinal problem, which we suggest calling lipid malabsorption syndrome (LMS), cha...
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crspringernat:10.1186/s12917-020-2252-7 2023-05-15T15:31:17+02:00 Choline supplementation prevents diet induced gut mucosa lipid accumulation in post-smolt Atlantic salmon (Salmo salar L.) Hansen, Anne Kristine G. Kortner, Trond M. Krasnov, Aleksei Björkhem, Ingemar Penn, Michael Krogdahl, Åshild The Research Council of Norway BioMar 2020 http://dx.doi.org/10.1186/s12917-020-2252-7 http://link.springer.com/content/pdf/10.1186/s12917-020-2252-7.pdf http://link.springer.com/article/10.1186/s12917-020-2252-7/fulltext.html en eng Springer Science and Business Media LLC http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/ CC-BY BMC Veterinary Research volume 16, issue 1 ISSN 1746-6148 General Veterinary General Medicine journal-article 2020 crspringernat https://doi.org/10.1186/s12917-020-2252-7 2022-01-04T11:01:04Z Abstract Background Various intestinal morphological alterations have been reported in cultured fish fed diets with high contents of plant ingredients. Since 2000, salmon farmers have reported symptoms indicating an intestinal problem, which we suggest calling lipid malabsorption syndrome (LMS), characterized by pale and foamy appearance of the enterocytes of the pyloric caeca, the result of lipid accumulation. The objective of the present study was to investigate if insufficient dietary choline may be a key component in development of the LMS. Results The results showed that Atlantic salmon ( Salmo salar ), average weight 362 g, fed a plant based diet for 79 days developed signs of LMS. In fish fed a similar diet supplemented with 0.4% choline chloride no signs of LMS were seen. The relative weight of the pyloric caeca was 40% lower, reflecting 65% less triacylglycerol content and histologically normal gut mucosa. Choline supplementation further increased specific fish growth by 18%. The concomitant alterations in intestinal gene expression related to phosphatidylcholine synthesis ( chk and pcyt1a ), cholesterol transport ( abcg5 and npc1l1 ), lipid metabolism and transport ( mgat2a and fabp2 ) and lipoprotein formation ( apoA1 and apoAIV ) confirmed the importance of choline in lipid turnover in the intestine and its ability to prevent LMS. Another important observation was the apparent correlation between plin2 expression and degree of enterocyte hyper-vacuolation observed in the current study, which suggests that plin2 may serve as a marker for intestinal lipid accumulation and steatosis in fish. Future research should be conducted to strengthen the knowledge of choline’s critical role in lipid transport, phospholipid synthesis and lipoprotein secretion to improve formulations of plant based diets for larger fish and to prevent LMS. Conclusions Choline prevents excessive lipid accumulation in the proximal intestine and is essential for Atlantic salmon in seawater. Article in Journal/Newspaper Atlantic salmon Salmo salar Springer Nature (via Crossref) BMC Veterinary Research 16 1 |
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English |
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General Veterinary General Medicine |
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General Veterinary General Medicine Hansen, Anne Kristine G. Kortner, Trond M. Krasnov, Aleksei Björkhem, Ingemar Penn, Michael Krogdahl, Åshild Choline supplementation prevents diet induced gut mucosa lipid accumulation in post-smolt Atlantic salmon (Salmo salar L.) |
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General Veterinary General Medicine |
description |
Abstract Background Various intestinal morphological alterations have been reported in cultured fish fed diets with high contents of plant ingredients. Since 2000, salmon farmers have reported symptoms indicating an intestinal problem, which we suggest calling lipid malabsorption syndrome (LMS), characterized by pale and foamy appearance of the enterocytes of the pyloric caeca, the result of lipid accumulation. The objective of the present study was to investigate if insufficient dietary choline may be a key component in development of the LMS. Results The results showed that Atlantic salmon ( Salmo salar ), average weight 362 g, fed a plant based diet for 79 days developed signs of LMS. In fish fed a similar diet supplemented with 0.4% choline chloride no signs of LMS were seen. The relative weight of the pyloric caeca was 40% lower, reflecting 65% less triacylglycerol content and histologically normal gut mucosa. Choline supplementation further increased specific fish growth by 18%. The concomitant alterations in intestinal gene expression related to phosphatidylcholine synthesis ( chk and pcyt1a ), cholesterol transport ( abcg5 and npc1l1 ), lipid metabolism and transport ( mgat2a and fabp2 ) and lipoprotein formation ( apoA1 and apoAIV ) confirmed the importance of choline in lipid turnover in the intestine and its ability to prevent LMS. Another important observation was the apparent correlation between plin2 expression and degree of enterocyte hyper-vacuolation observed in the current study, which suggests that plin2 may serve as a marker for intestinal lipid accumulation and steatosis in fish. Future research should be conducted to strengthen the knowledge of choline’s critical role in lipid transport, phospholipid synthesis and lipoprotein secretion to improve formulations of plant based diets for larger fish and to prevent LMS. Conclusions Choline prevents excessive lipid accumulation in the proximal intestine and is essential for Atlantic salmon in seawater. |
author2 |
The Research Council of Norway BioMar |
format |
Article in Journal/Newspaper |
author |
Hansen, Anne Kristine G. Kortner, Trond M. Krasnov, Aleksei Björkhem, Ingemar Penn, Michael Krogdahl, Åshild |
author_facet |
Hansen, Anne Kristine G. Kortner, Trond M. Krasnov, Aleksei Björkhem, Ingemar Penn, Michael Krogdahl, Åshild |
author_sort |
Hansen, Anne Kristine G. |
title |
Choline supplementation prevents diet induced gut mucosa lipid accumulation in post-smolt Atlantic salmon (Salmo salar L.) |
title_short |
Choline supplementation prevents diet induced gut mucosa lipid accumulation in post-smolt Atlantic salmon (Salmo salar L.) |
title_full |
Choline supplementation prevents diet induced gut mucosa lipid accumulation in post-smolt Atlantic salmon (Salmo salar L.) |
title_fullStr |
Choline supplementation prevents diet induced gut mucosa lipid accumulation in post-smolt Atlantic salmon (Salmo salar L.) |
title_full_unstemmed |
Choline supplementation prevents diet induced gut mucosa lipid accumulation in post-smolt Atlantic salmon (Salmo salar L.) |
title_sort |
choline supplementation prevents diet induced gut mucosa lipid accumulation in post-smolt atlantic salmon (salmo salar l.) |
publisher |
Springer Science and Business Media LLC |
publishDate |
2020 |
url |
http://dx.doi.org/10.1186/s12917-020-2252-7 http://link.springer.com/content/pdf/10.1186/s12917-020-2252-7.pdf http://link.springer.com/article/10.1186/s12917-020-2252-7/fulltext.html |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
BMC Veterinary Research volume 16, issue 1 ISSN 1746-6148 |
op_rights |
http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/ |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1186/s12917-020-2252-7 |
container_title |
BMC Veterinary Research |
container_volume |
16 |
container_issue |
1 |
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1766361768849833984 |