Expression of transport proteins in the rete mirabile of european silver and yellow eel
Abstract Background In physoclist fishes filling of the swimbladder requires acid secretion of gas gland cells to switch on the Root effect and subsequent countercurrent concentration of the initial gas partial pressure increase by back-diffusion of gas molecules in the rete mirabile. It is generall...
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crspringernat:10.1186/s12864-021-08180-2 2023-05-15T16:08:42+02:00 Expression of transport proteins in the rete mirabile of european silver and yellow eel Schneebauer, Gabriel Drechsel, Victoria Dirks, Ron Faserl, Klaus Sarg, Bettina Pelster, Bernd Austrian Science Foundation FWF FWF 2021 http://dx.doi.org/10.1186/s12864-021-08180-2 https://link.springer.com/content/pdf/10.1186/s12864-021-08180-2.pdf https://link.springer.com/article/10.1186/s12864-021-08180-2/fulltext.html en eng Springer Science and Business Media LLC https://creativecommons.org/licenses/by/4.0 https://creativecommons.org/licenses/by/4.0 CC-BY BMC Genomics volume 22, issue 1 ISSN 1471-2164 Genetics Biotechnology journal-article 2021 crspringernat https://doi.org/10.1186/s12864-021-08180-2 2022-01-04T07:15:29Z Abstract Background In physoclist fishes filling of the swimbladder requires acid secretion of gas gland cells to switch on the Root effect and subsequent countercurrent concentration of the initial gas partial pressure increase by back-diffusion of gas molecules in the rete mirabile. It is generally assumed that the rete mirabile functions as a passive exchanger, but a detailed analysis of lactate and water movements in the rete mirabile of the eel revealed that lactate is diffusing back in the rete. In the present study we therefore test the hypothesis that expression of transport proteins in rete capillaries allows for back-diffusion of ions and metabolites, which would support the countercurrent concentrating capacity of the rete mirabile. It is also assumed that in silver eels, the migratory stage of the eel, the expression of transport proteins would be enhanced. Results Analysis of the transcriptome and of the proteome of rete mirabile tissue of the European eel revealed the expression of a large number of membrane ion and metabolite transport proteins, including monocarboxylate and glucose transport proteins. In addition, ion channel proteins, Ca 2+ -ATPase, Na + /K + -ATPase and also F 1 F 0 -ATP synthase were detected. In contrast to our expectation in silver eels the expression of these transport proteins was not elevated as compared to yellow eels. A remarkable number of enzymes degrading reactive oxygen species (ROS) was detected in rete capillaries. Conclusions Our results reveal the expression of a large number of transport proteins in rete capillaries, so that the back diffusion of ions and metabolites, in particular lactate, may significantly enhance the countercurrent concentrating ability of the rete. Metabolic pathways allowing for aerobic generation of ATP supporting secondary active transport mechanisms are established. Rete tissue appears to be equipped with a high ROS defense capacity, preventing damage of the tissue due to the high oxygen partial pressures generated in the countercurrent system. Article in Journal/Newspaper European eel Springer Nature (via Crossref) BMC Genomics 22 1 |
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Springer Nature (via Crossref) |
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English |
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Genetics Biotechnology |
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Genetics Biotechnology Schneebauer, Gabriel Drechsel, Victoria Dirks, Ron Faserl, Klaus Sarg, Bettina Pelster, Bernd Expression of transport proteins in the rete mirabile of european silver and yellow eel |
topic_facet |
Genetics Biotechnology |
description |
Abstract Background In physoclist fishes filling of the swimbladder requires acid secretion of gas gland cells to switch on the Root effect and subsequent countercurrent concentration of the initial gas partial pressure increase by back-diffusion of gas molecules in the rete mirabile. It is generally assumed that the rete mirabile functions as a passive exchanger, but a detailed analysis of lactate and water movements in the rete mirabile of the eel revealed that lactate is diffusing back in the rete. In the present study we therefore test the hypothesis that expression of transport proteins in rete capillaries allows for back-diffusion of ions and metabolites, which would support the countercurrent concentrating capacity of the rete mirabile. It is also assumed that in silver eels, the migratory stage of the eel, the expression of transport proteins would be enhanced. Results Analysis of the transcriptome and of the proteome of rete mirabile tissue of the European eel revealed the expression of a large number of membrane ion and metabolite transport proteins, including monocarboxylate and glucose transport proteins. In addition, ion channel proteins, Ca 2+ -ATPase, Na + /K + -ATPase and also F 1 F 0 -ATP synthase were detected. In contrast to our expectation in silver eels the expression of these transport proteins was not elevated as compared to yellow eels. A remarkable number of enzymes degrading reactive oxygen species (ROS) was detected in rete capillaries. Conclusions Our results reveal the expression of a large number of transport proteins in rete capillaries, so that the back diffusion of ions and metabolites, in particular lactate, may significantly enhance the countercurrent concentrating ability of the rete. Metabolic pathways allowing for aerobic generation of ATP supporting secondary active transport mechanisms are established. Rete tissue appears to be equipped with a high ROS defense capacity, preventing damage of the tissue due to the high oxygen partial pressures generated in the countercurrent system. |
author2 |
Austrian Science Foundation FWF FWF |
format |
Article in Journal/Newspaper |
author |
Schneebauer, Gabriel Drechsel, Victoria Dirks, Ron Faserl, Klaus Sarg, Bettina Pelster, Bernd |
author_facet |
Schneebauer, Gabriel Drechsel, Victoria Dirks, Ron Faserl, Klaus Sarg, Bettina Pelster, Bernd |
author_sort |
Schneebauer, Gabriel |
title |
Expression of transport proteins in the rete mirabile of european silver and yellow eel |
title_short |
Expression of transport proteins in the rete mirabile of european silver and yellow eel |
title_full |
Expression of transport proteins in the rete mirabile of european silver and yellow eel |
title_fullStr |
Expression of transport proteins in the rete mirabile of european silver and yellow eel |
title_full_unstemmed |
Expression of transport proteins in the rete mirabile of european silver and yellow eel |
title_sort |
expression of transport proteins in the rete mirabile of european silver and yellow eel |
publisher |
Springer Science and Business Media LLC |
publishDate |
2021 |
url |
http://dx.doi.org/10.1186/s12864-021-08180-2 https://link.springer.com/content/pdf/10.1186/s12864-021-08180-2.pdf https://link.springer.com/article/10.1186/s12864-021-08180-2/fulltext.html |
genre |
European eel |
genre_facet |
European eel |
op_source |
BMC Genomics volume 22, issue 1 ISSN 1471-2164 |
op_rights |
https://creativecommons.org/licenses/by/4.0 https://creativecommons.org/licenses/by/4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1186/s12864-021-08180-2 |
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BMC Genomics |
container_volume |
22 |
container_issue |
1 |
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1766404721474535424 |