Comparative transcriptome analysis of the newly discovered insect vector of the pine wood nematode in China, revealing putative genes related to host plant adaptation
Abstract Background In many insect species, the larvae/nymphs are unable to disperse far from the oviposition site selected by adults. The Sakhalin pine sawyer Monochamus saltuarius (Gebler) is the newly discovered insect vector of the pine wood nematode ( Bursaphelenchus xylophilus ) in China. Adul...
Published in: | BMC Genomics |
---|---|
Main Authors: | , , , , , , |
Other Authors: | , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Springer Science and Business Media LLC
2021
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1186/s12864-021-07498-1 https://link.springer.com/content/pdf/10.1186/s12864-021-07498-1.pdf https://link.springer.com/article/10.1186/s12864-021-07498-1/fulltext.html |
id |
crspringernat:10.1186/s12864-021-07498-1 |
---|---|
record_format |
openpolar |
spelling |
crspringernat:10.1186/s12864-021-07498-1 2023-05-15T18:09:18+02:00 Comparative transcriptome analysis of the newly discovered insect vector of the pine wood nematode in China, revealing putative genes related to host plant adaptation Hou, Zehai Shi, Fengming Ge, Sixun Tao, Jing Ren, Lili Wu, Hao Zong, Shixiang Fundamental Research Funds for the Central Universities National Key Research and Development Program of China Major Project of Liaoning Province Programs for Fundamental Research and Development 2021 http://dx.doi.org/10.1186/s12864-021-07498-1 https://link.springer.com/content/pdf/10.1186/s12864-021-07498-1.pdf https://link.springer.com/article/10.1186/s12864-021-07498-1/fulltext.html en eng Springer Science and Business Media LLC http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/ CC-BY BMC Genomics volume 22, issue 1 ISSN 1471-2164 Genetics Biotechnology journal-article 2021 crspringernat https://doi.org/10.1186/s12864-021-07498-1 2022-01-04T16:04:30Z Abstract Background In many insect species, the larvae/nymphs are unable to disperse far from the oviposition site selected by adults. The Sakhalin pine sawyer Monochamus saltuarius (Gebler) is the newly discovered insect vector of the pine wood nematode ( Bursaphelenchus xylophilus ) in China. Adult M. saltuarius prefers to oviposit on the host plant Pinus koraiensis , rather than P. tabuliformis . However, the genetic basis of adaptation of the larvae of M. saltuarius with weaken dispersal ability to host environments selected by the adult is not well understood. Results In this study, the free amino and fatty acid composition and content of the host plants of M. saltuarius larvae, i.e., P. koraiensis and P. tabuliformis were investigated . Compared with P. koraiensis , P. tabuliformis had a substantially higher content of various free amino acids, while the opposite trend was detected for fatty acid content. The transcriptional profiles of larval populations feeding on P. koraiensis and P. tabuliformis were compared using PacBio Sequel II sequencing combined with Illumina sequencing. The results showed that genes relating to digestion, fatty acid synthesis, detoxification, oxidation-reduction, and stress response, as well as nutrients and energy sensing ability, were differentially expressed, possibly reflecting adaptive changes of M. saltuarius in response to different host diets. Additionally, genes coding for cuticle structure were differentially expressed, indicating that cuticle may be a potential target for plant defense. Differential regulation of genes related to the antibacterial and immune response were also observed, suggesting that larvae of M. saltuarius may have evolved adaptations to cope with bacterial challenges in their host environments. Conclusions The present study provides comprehensive transcriptome resource of M. saltuarius relating to host plant adaptation. Results from this study help to illustrate the fundamental relationship between transcriptional plasticity and adaptation mechanisms of insect herbivores to host plants. Article in Journal/Newspaper Sakhalin Springer Nature (via Crossref) BMC Genomics 22 1 |
institution |
Open Polar |
collection |
Springer Nature (via Crossref) |
op_collection_id |
crspringernat |
language |
English |
topic |
Genetics Biotechnology |
spellingShingle |
Genetics Biotechnology Hou, Zehai Shi, Fengming Ge, Sixun Tao, Jing Ren, Lili Wu, Hao Zong, Shixiang Comparative transcriptome analysis of the newly discovered insect vector of the pine wood nematode in China, revealing putative genes related to host plant adaptation |
topic_facet |
Genetics Biotechnology |
description |
Abstract Background In many insect species, the larvae/nymphs are unable to disperse far from the oviposition site selected by adults. The Sakhalin pine sawyer Monochamus saltuarius (Gebler) is the newly discovered insect vector of the pine wood nematode ( Bursaphelenchus xylophilus ) in China. Adult M. saltuarius prefers to oviposit on the host plant Pinus koraiensis , rather than P. tabuliformis . However, the genetic basis of adaptation of the larvae of M. saltuarius with weaken dispersal ability to host environments selected by the adult is not well understood. Results In this study, the free amino and fatty acid composition and content of the host plants of M. saltuarius larvae, i.e., P. koraiensis and P. tabuliformis were investigated . Compared with P. koraiensis , P. tabuliformis had a substantially higher content of various free amino acids, while the opposite trend was detected for fatty acid content. The transcriptional profiles of larval populations feeding on P. koraiensis and P. tabuliformis were compared using PacBio Sequel II sequencing combined with Illumina sequencing. The results showed that genes relating to digestion, fatty acid synthesis, detoxification, oxidation-reduction, and stress response, as well as nutrients and energy sensing ability, were differentially expressed, possibly reflecting adaptive changes of M. saltuarius in response to different host diets. Additionally, genes coding for cuticle structure were differentially expressed, indicating that cuticle may be a potential target for plant defense. Differential regulation of genes related to the antibacterial and immune response were also observed, suggesting that larvae of M. saltuarius may have evolved adaptations to cope with bacterial challenges in their host environments. Conclusions The present study provides comprehensive transcriptome resource of M. saltuarius relating to host plant adaptation. Results from this study help to illustrate the fundamental relationship between transcriptional plasticity and adaptation mechanisms of insect herbivores to host plants. |
author2 |
Fundamental Research Funds for the Central Universities National Key Research and Development Program of China Major Project of Liaoning Province Programs for Fundamental Research and Development |
format |
Article in Journal/Newspaper |
author |
Hou, Zehai Shi, Fengming Ge, Sixun Tao, Jing Ren, Lili Wu, Hao Zong, Shixiang |
author_facet |
Hou, Zehai Shi, Fengming Ge, Sixun Tao, Jing Ren, Lili Wu, Hao Zong, Shixiang |
author_sort |
Hou, Zehai |
title |
Comparative transcriptome analysis of the newly discovered insect vector of the pine wood nematode in China, revealing putative genes related to host plant adaptation |
title_short |
Comparative transcriptome analysis of the newly discovered insect vector of the pine wood nematode in China, revealing putative genes related to host plant adaptation |
title_full |
Comparative transcriptome analysis of the newly discovered insect vector of the pine wood nematode in China, revealing putative genes related to host plant adaptation |
title_fullStr |
Comparative transcriptome analysis of the newly discovered insect vector of the pine wood nematode in China, revealing putative genes related to host plant adaptation |
title_full_unstemmed |
Comparative transcriptome analysis of the newly discovered insect vector of the pine wood nematode in China, revealing putative genes related to host plant adaptation |
title_sort |
comparative transcriptome analysis of the newly discovered insect vector of the pine wood nematode in china, revealing putative genes related to host plant adaptation |
publisher |
Springer Science and Business Media LLC |
publishDate |
2021 |
url |
http://dx.doi.org/10.1186/s12864-021-07498-1 https://link.springer.com/content/pdf/10.1186/s12864-021-07498-1.pdf https://link.springer.com/article/10.1186/s12864-021-07498-1/fulltext.html |
genre |
Sakhalin |
genre_facet |
Sakhalin |
op_source |
BMC Genomics volume 22, issue 1 ISSN 1471-2164 |
op_rights |
http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/ |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1186/s12864-021-07498-1 |
container_title |
BMC Genomics |
container_volume |
22 |
container_issue |
1 |
_version_ |
1766181786611613696 |