Transcriptomic analysis of dead end knockout testis reveals germ cell and gonadal somatic factors in Atlantic salmon
Abstract Background Sustainability challenges are currently hampering an increase in salmon production. Using sterile salmon can solve problems with precocious puberty and genetic introgression from farmed escapees to wild populations. Recently sterile salmon was produced by knocking out the germ ce...
Published in: | BMC Genomics |
---|---|
Main Authors: | , , , , , , |
Other Authors: | |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Springer Science and Business Media LLC
2020
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1186/s12864-020-6513-4 http://link.springer.com/content/pdf/10.1186/s12864-020-6513-4.pdf http://link.springer.com/article/10.1186/s12864-020-6513-4/fulltext.html |
id |
crspringernat:10.1186/s12864-020-6513-4 |
---|---|
record_format |
openpolar |
spelling |
crspringernat:10.1186/s12864-020-6513-4 2023-05-15T15:32:17+02:00 Transcriptomic analysis of dead end knockout testis reveals germ cell and gonadal somatic factors in Atlantic salmon Kleppe, Lene Edvardsen, Rolf Brudvik Furmanek, Tomasz Andersson, Eva Skaftnesmo, Kai Ove Thyri Segafredo, Frida Wargelius, Anna Norges Forskningsråd 2020 http://dx.doi.org/10.1186/s12864-020-6513-4 http://link.springer.com/content/pdf/10.1186/s12864-020-6513-4.pdf http://link.springer.com/article/10.1186/s12864-020-6513-4/fulltext.html en eng Springer Science and Business Media LLC http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/ CC-BY BMC Genomics volume 21, issue 1 ISSN 1471-2164 Genetics Biotechnology journal-article 2020 crspringernat https://doi.org/10.1186/s12864-020-6513-4 2022-01-04T16:31:00Z Abstract Background Sustainability challenges are currently hampering an increase in salmon production. Using sterile salmon can solve problems with precocious puberty and genetic introgression from farmed escapees to wild populations. Recently sterile salmon was produced by knocking out the germ cell-specific dead end ( dnd ). Several approaches may be applied to inhibit Dnd function, including gene knockout, knockdown or immunization. Since it is challenging to develop a successful treatment against a gene product already existing in the body, alternative targets are being explored. Germ cells are surrounded by, and dependent on, gonadal somatic cells. Targeting genes essential for the survival of gonadal somatic cells may be good alternative targets for sterility treatments. Our aim was to identify and characterize novel germ cell and gonadal somatic factors in Atlantic salmon. Results We have for the first time analysed RNA-sequencing data from germ cell-free (GCF)/ dnd knockout and wild type (WT) salmon testis and searched for genes preferentially expressed in either germ cells or gonadal somatic cells. To exclude genes with extra-gonadal expression, our dataset was merged with available multi-tissue transcriptome data. We identified 389 gonad specific genes, of which 194 were preferentially expressed within germ cells, and 11 were confined to gonadal somatic cells. Interestingly, 5 of the 11 gonadal somatic transcripts represented genes encoding secreted TGF-β factors; gsdf , inha , nodal and two bmp6-like genes, all representative vaccine targets. Of these, gsdf and inha had the highest transcript levels. Expression of gsdf and inha was further confirmed to be gonad specific, and their spatial expression was restricted to granulosa and Sertoli cells of the ovary and testis, respectively. Finally, we show that inha expression increases with puberty in both ovary and testis tissue, while gsdf expression does not change or decreases during puberty in ovary and testis tissue, respectively. Conclusions This study contributes with transcriptome data on salmon testis tissue with and without germ cells. We provide a list of novel and known germ cell- and gonad somatic specific transcripts, and show that the expression of two highly active gonadal somatic secreted TGF-β factors, gsdf and inha , are located within granulosa and Sertoli cells. Article in Journal/Newspaper Atlantic salmon Springer Nature (via Crossref) BMC Genomics 21 1 |
institution |
Open Polar |
collection |
Springer Nature (via Crossref) |
op_collection_id |
crspringernat |
language |
English |
topic |
Genetics Biotechnology |
spellingShingle |
Genetics Biotechnology Kleppe, Lene Edvardsen, Rolf Brudvik Furmanek, Tomasz Andersson, Eva Skaftnesmo, Kai Ove Thyri Segafredo, Frida Wargelius, Anna Transcriptomic analysis of dead end knockout testis reveals germ cell and gonadal somatic factors in Atlantic salmon |
topic_facet |
Genetics Biotechnology |
description |
Abstract Background Sustainability challenges are currently hampering an increase in salmon production. Using sterile salmon can solve problems with precocious puberty and genetic introgression from farmed escapees to wild populations. Recently sterile salmon was produced by knocking out the germ cell-specific dead end ( dnd ). Several approaches may be applied to inhibit Dnd function, including gene knockout, knockdown or immunization. Since it is challenging to develop a successful treatment against a gene product already existing in the body, alternative targets are being explored. Germ cells are surrounded by, and dependent on, gonadal somatic cells. Targeting genes essential for the survival of gonadal somatic cells may be good alternative targets for sterility treatments. Our aim was to identify and characterize novel germ cell and gonadal somatic factors in Atlantic salmon. Results We have for the first time analysed RNA-sequencing data from germ cell-free (GCF)/ dnd knockout and wild type (WT) salmon testis and searched for genes preferentially expressed in either germ cells or gonadal somatic cells. To exclude genes with extra-gonadal expression, our dataset was merged with available multi-tissue transcriptome data. We identified 389 gonad specific genes, of which 194 were preferentially expressed within germ cells, and 11 were confined to gonadal somatic cells. Interestingly, 5 of the 11 gonadal somatic transcripts represented genes encoding secreted TGF-β factors; gsdf , inha , nodal and two bmp6-like genes, all representative vaccine targets. Of these, gsdf and inha had the highest transcript levels. Expression of gsdf and inha was further confirmed to be gonad specific, and their spatial expression was restricted to granulosa and Sertoli cells of the ovary and testis, respectively. Finally, we show that inha expression increases with puberty in both ovary and testis tissue, while gsdf expression does not change or decreases during puberty in ovary and testis tissue, respectively. Conclusions This study contributes with transcriptome data on salmon testis tissue with and without germ cells. We provide a list of novel and known germ cell- and gonad somatic specific transcripts, and show that the expression of two highly active gonadal somatic secreted TGF-β factors, gsdf and inha , are located within granulosa and Sertoli cells. |
author2 |
Norges Forskningsråd |
format |
Article in Journal/Newspaper |
author |
Kleppe, Lene Edvardsen, Rolf Brudvik Furmanek, Tomasz Andersson, Eva Skaftnesmo, Kai Ove Thyri Segafredo, Frida Wargelius, Anna |
author_facet |
Kleppe, Lene Edvardsen, Rolf Brudvik Furmanek, Tomasz Andersson, Eva Skaftnesmo, Kai Ove Thyri Segafredo, Frida Wargelius, Anna |
author_sort |
Kleppe, Lene |
title |
Transcriptomic analysis of dead end knockout testis reveals germ cell and gonadal somatic factors in Atlantic salmon |
title_short |
Transcriptomic analysis of dead end knockout testis reveals germ cell and gonadal somatic factors in Atlantic salmon |
title_full |
Transcriptomic analysis of dead end knockout testis reveals germ cell and gonadal somatic factors in Atlantic salmon |
title_fullStr |
Transcriptomic analysis of dead end knockout testis reveals germ cell and gonadal somatic factors in Atlantic salmon |
title_full_unstemmed |
Transcriptomic analysis of dead end knockout testis reveals germ cell and gonadal somatic factors in Atlantic salmon |
title_sort |
transcriptomic analysis of dead end knockout testis reveals germ cell and gonadal somatic factors in atlantic salmon |
publisher |
Springer Science and Business Media LLC |
publishDate |
2020 |
url |
http://dx.doi.org/10.1186/s12864-020-6513-4 http://link.springer.com/content/pdf/10.1186/s12864-020-6513-4.pdf http://link.springer.com/article/10.1186/s12864-020-6513-4/fulltext.html |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
BMC Genomics volume 21, issue 1 ISSN 1471-2164 |
op_rights |
http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/ |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1186/s12864-020-6513-4 |
container_title |
BMC Genomics |
container_volume |
21 |
container_issue |
1 |
_version_ |
1766362802326339584 |