Parallel analysis of miRNAs and mRNAs suggests distinct regulatory networks in Crassostrea gigas infected by Ostreid herpesvirus 1
Abstract Background Since 2008, the aquaculture production of Crassostrea gigas was heavily affected by mass mortalities associated to Ostreid herpesvirus 1 (OsHV-1) microvariants worldwide. Transcriptomic studies revealed the major antiviral pathways of the oyster immune response while other findin...
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crspringernat:10.1186/s12864-020-07026-7 2023-05-15T15:58:17+02:00 Parallel analysis of miRNAs and mRNAs suggests distinct regulatory networks in Crassostrea gigas infected by Ostreid herpesvirus 1 Rosani, Umberto Abbadi, Miriam Green, Timothy Bai, Chang-Ming Turolla, Edoardo Arcangeli, Giuseppe Wegner, K. Mathias Venier, Paola 2020 http://dx.doi.org/10.1186/s12864-020-07026-7 https://link.springer.com/content/pdf/10.1186/s12864-020-07026-7.pdf https://link.springer.com/article/10.1186/s12864-020-07026-7/fulltext.html en eng Springer Science and Business Media LLC https://creativecommons.org/licenses/by/4.0 https://creativecommons.org/licenses/by/4.0 CC-BY BMC Genomics volume 21, issue 1 ISSN 1471-2164 Genetics Biotechnology journal-article 2020 crspringernat https://doi.org/10.1186/s12864-020-07026-7 2022-01-04T16:51:18Z Abstract Background Since 2008, the aquaculture production of Crassostrea gigas was heavily affected by mass mortalities associated to Ostreid herpesvirus 1 (OsHV-1) microvariants worldwide. Transcriptomic studies revealed the major antiviral pathways of the oyster immune response while other findings suggested that also small non-coding RNAs (sncRNA) such as microRNAs might act as key regulators of the oyster response against OsHV-1. To explore the explicit connection between small non-coding and protein-coding transcripts, we performed paired whole transcriptome analysis of sncRNA and messenger RNA (mRNA) in six oysters selected for different intensities of OsHV-1 infection. Results The mRNA profiles of the naturally infected oysters were mostly governed by the transcriptional activity of OsHV-1, with several differentially expressed genes mapping to the interferon, toll, apoptosis, and pro-PO pathways. In contrast, miRNA profiles suggested more complex regulatory mechanisms, with 15 differentially expressed miRNAs (DE-miRNA) pointing to a possible modulation of the host response during OsHV-1 infection. We predicted 68 interactions between DE-miRNAs and oyster 3′-UTRs, but only few of them involved antiviral genes. The sncRNA reads assigned to OsHV-1 rather resembled mRNA degradation products, suggesting the absence of genuine viral miRNAs. Conclusions We provided data describing the miRNAome during OsHV-1 infection in C. gigas . This information can be used to understand the role of miRNAs in healthy and diseased oysters, to identify new targets for functional studies and, eventually to disentangle cause and effect relationships during viral infections in marine mollusks. Article in Journal/Newspaper Crassostrea gigas Springer Nature (via Crossref) BMC Genomics 21 1 |
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Springer Nature (via Crossref) |
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English |
topic |
Genetics Biotechnology |
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Genetics Biotechnology Rosani, Umberto Abbadi, Miriam Green, Timothy Bai, Chang-Ming Turolla, Edoardo Arcangeli, Giuseppe Wegner, K. Mathias Venier, Paola Parallel analysis of miRNAs and mRNAs suggests distinct regulatory networks in Crassostrea gigas infected by Ostreid herpesvirus 1 |
topic_facet |
Genetics Biotechnology |
description |
Abstract Background Since 2008, the aquaculture production of Crassostrea gigas was heavily affected by mass mortalities associated to Ostreid herpesvirus 1 (OsHV-1) microvariants worldwide. Transcriptomic studies revealed the major antiviral pathways of the oyster immune response while other findings suggested that also small non-coding RNAs (sncRNA) such as microRNAs might act as key regulators of the oyster response against OsHV-1. To explore the explicit connection between small non-coding and protein-coding transcripts, we performed paired whole transcriptome analysis of sncRNA and messenger RNA (mRNA) in six oysters selected for different intensities of OsHV-1 infection. Results The mRNA profiles of the naturally infected oysters were mostly governed by the transcriptional activity of OsHV-1, with several differentially expressed genes mapping to the interferon, toll, apoptosis, and pro-PO pathways. In contrast, miRNA profiles suggested more complex regulatory mechanisms, with 15 differentially expressed miRNAs (DE-miRNA) pointing to a possible modulation of the host response during OsHV-1 infection. We predicted 68 interactions between DE-miRNAs and oyster 3′-UTRs, but only few of them involved antiviral genes. The sncRNA reads assigned to OsHV-1 rather resembled mRNA degradation products, suggesting the absence of genuine viral miRNAs. Conclusions We provided data describing the miRNAome during OsHV-1 infection in C. gigas . This information can be used to understand the role of miRNAs in healthy and diseased oysters, to identify new targets for functional studies and, eventually to disentangle cause and effect relationships during viral infections in marine mollusks. |
format |
Article in Journal/Newspaper |
author |
Rosani, Umberto Abbadi, Miriam Green, Timothy Bai, Chang-Ming Turolla, Edoardo Arcangeli, Giuseppe Wegner, K. Mathias Venier, Paola |
author_facet |
Rosani, Umberto Abbadi, Miriam Green, Timothy Bai, Chang-Ming Turolla, Edoardo Arcangeli, Giuseppe Wegner, K. Mathias Venier, Paola |
author_sort |
Rosani, Umberto |
title |
Parallel analysis of miRNAs and mRNAs suggests distinct regulatory networks in Crassostrea gigas infected by Ostreid herpesvirus 1 |
title_short |
Parallel analysis of miRNAs and mRNAs suggests distinct regulatory networks in Crassostrea gigas infected by Ostreid herpesvirus 1 |
title_full |
Parallel analysis of miRNAs and mRNAs suggests distinct regulatory networks in Crassostrea gigas infected by Ostreid herpesvirus 1 |
title_fullStr |
Parallel analysis of miRNAs and mRNAs suggests distinct regulatory networks in Crassostrea gigas infected by Ostreid herpesvirus 1 |
title_full_unstemmed |
Parallel analysis of miRNAs and mRNAs suggests distinct regulatory networks in Crassostrea gigas infected by Ostreid herpesvirus 1 |
title_sort |
parallel analysis of mirnas and mrnas suggests distinct regulatory networks in crassostrea gigas infected by ostreid herpesvirus 1 |
publisher |
Springer Science and Business Media LLC |
publishDate |
2020 |
url |
http://dx.doi.org/10.1186/s12864-020-07026-7 https://link.springer.com/content/pdf/10.1186/s12864-020-07026-7.pdf https://link.springer.com/article/10.1186/s12864-020-07026-7/fulltext.html |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
BMC Genomics volume 21, issue 1 ISSN 1471-2164 |
op_rights |
https://creativecommons.org/licenses/by/4.0 https://creativecommons.org/licenses/by/4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1186/s12864-020-07026-7 |
container_title |
BMC Genomics |
container_volume |
21 |
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1 |
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1766394015002918912 |