Chromosome aberrations in pressure-induced triploid Atlantic salmon
Abstract Background Triploid organisms have three sets of chromosomes. In Atlantic salmon, hydrostatic pressure treatment of newly fertilized eggs has been extensively used to produce triploids which are functionally sterile due to their unpaired chromosomes. These fish often perform poorly on comme...
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crspringernat:10.1186/s12863-020-00864-0 2023-05-15T15:30:01+02:00 Chromosome aberrations in pressure-induced triploid Atlantic salmon Glover, K. A. Harvey, A. C. Hansen, T. J. Fjelldal, P. G. Besnier, F. N. Bos, J. B. Ayllon, F. Taggart, J. B. Solberg, M. F. Nærings- og Fiskeridepartementet 2020 http://dx.doi.org/10.1186/s12863-020-00864-0 https://link.springer.com/content/pdf/10.1186/s12863-020-00864-0.pdf https://link.springer.com/article/10.1186/s12863-020-00864-0/fulltext.html en eng Springer Science and Business Media LLC https://creativecommons.org/licenses/by/4.0 https://creativecommons.org/licenses/by/4.0 CC-BY BMC Genetics volume 21, issue 1 ISSN 1471-2156 Genetics(clinical) Genetics journal-article 2020 crspringernat https://doi.org/10.1186/s12863-020-00864-0 2022-01-04T09:05:55Z Abstract Background Triploid organisms have three sets of chromosomes. In Atlantic salmon, hydrostatic pressure treatment of newly fertilized eggs has been extensively used to produce triploids which are functionally sterile due to their unpaired chromosomes. These fish often perform poorly on commercial farms, sometimes without explanation. Inheritance patterns in individuals subjected to pressure treatment have not been investigated in Atlantic salmon thus far. However, work on other species suggests that this treatment can result in aberrant inheritance. We therefore studied this in Atlantic salmon by genotyping 16 polymorphic microsatellites in eyed eggs and juveniles which had been subjected to pressure-induction of triploidy. Communally reared juveniles including fish subjected to pressure-induction of triploidy and their diploid siblings were included as a control. Results No diploid offspring were detected in any of the eggs or juveniles which were subjected to hydrostatic pressure; therefore, the induction of triploidy was highly successful. Aberrant inheritance was nevertheless observed in 0.9% of the eggs and 0.9% of the juveniles that had been subjected to pressure treatment. In the communally reared fish, 0.3% of the fish subjected to pressure treatment displayed aberrant inheritance, while their diploid controls displayed 0% aberrant inheritance. Inheritance errors included two eyed eggs lacking maternal DNA across all microsatellites, and, examples in both eggs and juveniles of either the maternal or paternal allele lacking in one of the microsatellites. All individuals displaying chromosome aberrations were otherwise triploid. Conclusions This is the first study to document aberrant inheritance in Atlantic salmon that have been subjected to pressure-induction of triploidy. Our experiments unequivocally demonstrate that even when induction of triploidy is highly successful, this treatment can cause chromosome aberrations in this species. Based upon our novel data, and earlier studies in other organisms, we hypothesize that in batches of Atlantic salmon where low to modest triploid induction rates have been reported, aberrant inheritance is likely to be higher than the rates observed here. Therefore, we tentatively suggest that this could contribute to the unexplained poor performance of triploid salmon that is occasionally reported in commercial aquaculture. These hypotheses require further investigation. Article in Journal/Newspaper Atlantic salmon Springer Nature (via Crossref) BMC Genetics 21 1 |
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Open Polar |
collection |
Springer Nature (via Crossref) |
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crspringernat |
language |
English |
topic |
Genetics(clinical) Genetics |
spellingShingle |
Genetics(clinical) Genetics Glover, K. A. Harvey, A. C. Hansen, T. J. Fjelldal, P. G. Besnier, F. N. Bos, J. B. Ayllon, F. Taggart, J. B. Solberg, M. F. Chromosome aberrations in pressure-induced triploid Atlantic salmon |
topic_facet |
Genetics(clinical) Genetics |
description |
Abstract Background Triploid organisms have three sets of chromosomes. In Atlantic salmon, hydrostatic pressure treatment of newly fertilized eggs has been extensively used to produce triploids which are functionally sterile due to their unpaired chromosomes. These fish often perform poorly on commercial farms, sometimes without explanation. Inheritance patterns in individuals subjected to pressure treatment have not been investigated in Atlantic salmon thus far. However, work on other species suggests that this treatment can result in aberrant inheritance. We therefore studied this in Atlantic salmon by genotyping 16 polymorphic microsatellites in eyed eggs and juveniles which had been subjected to pressure-induction of triploidy. Communally reared juveniles including fish subjected to pressure-induction of triploidy and their diploid siblings were included as a control. Results No diploid offspring were detected in any of the eggs or juveniles which were subjected to hydrostatic pressure; therefore, the induction of triploidy was highly successful. Aberrant inheritance was nevertheless observed in 0.9% of the eggs and 0.9% of the juveniles that had been subjected to pressure treatment. In the communally reared fish, 0.3% of the fish subjected to pressure treatment displayed aberrant inheritance, while their diploid controls displayed 0% aberrant inheritance. Inheritance errors included two eyed eggs lacking maternal DNA across all microsatellites, and, examples in both eggs and juveniles of either the maternal or paternal allele lacking in one of the microsatellites. All individuals displaying chromosome aberrations were otherwise triploid. Conclusions This is the first study to document aberrant inheritance in Atlantic salmon that have been subjected to pressure-induction of triploidy. Our experiments unequivocally demonstrate that even when induction of triploidy is highly successful, this treatment can cause chromosome aberrations in this species. Based upon our novel data, and earlier studies in other organisms, we hypothesize that in batches of Atlantic salmon where low to modest triploid induction rates have been reported, aberrant inheritance is likely to be higher than the rates observed here. Therefore, we tentatively suggest that this could contribute to the unexplained poor performance of triploid salmon that is occasionally reported in commercial aquaculture. These hypotheses require further investigation. |
author2 |
Nærings- og Fiskeridepartementet |
format |
Article in Journal/Newspaper |
author |
Glover, K. A. Harvey, A. C. Hansen, T. J. Fjelldal, P. G. Besnier, F. N. Bos, J. B. Ayllon, F. Taggart, J. B. Solberg, M. F. |
author_facet |
Glover, K. A. Harvey, A. C. Hansen, T. J. Fjelldal, P. G. Besnier, F. N. Bos, J. B. Ayllon, F. Taggart, J. B. Solberg, M. F. |
author_sort |
Glover, K. A. |
title |
Chromosome aberrations in pressure-induced triploid Atlantic salmon |
title_short |
Chromosome aberrations in pressure-induced triploid Atlantic salmon |
title_full |
Chromosome aberrations in pressure-induced triploid Atlantic salmon |
title_fullStr |
Chromosome aberrations in pressure-induced triploid Atlantic salmon |
title_full_unstemmed |
Chromosome aberrations in pressure-induced triploid Atlantic salmon |
title_sort |
chromosome aberrations in pressure-induced triploid atlantic salmon |
publisher |
Springer Science and Business Media LLC |
publishDate |
2020 |
url |
http://dx.doi.org/10.1186/s12863-020-00864-0 https://link.springer.com/content/pdf/10.1186/s12863-020-00864-0.pdf https://link.springer.com/article/10.1186/s12863-020-00864-0/fulltext.html |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
BMC Genetics volume 21, issue 1 ISSN 1471-2156 |
op_rights |
https://creativecommons.org/licenses/by/4.0 https://creativecommons.org/licenses/by/4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1186/s12863-020-00864-0 |
container_title |
BMC Genetics |
container_volume |
21 |
container_issue |
1 |
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1766360471356571648 |