New cryptic species of the ‘revolutum’ group of Echinostoma (Digenea: Echinostomatidae) revealed by molecular and morphological data
Abstract Background The digenean species of Echinostoma (Echinostomatidae) with 37 collar spines that comprise the so-called ‘ revolutum ’ species complex, qualify as cryptic due to the interspecific homogeneity of characters used to differentiate species. Only five species were considered valid in...
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crspringernat:10.1186/1756-3305-6-64 2023-05-15T16:52:57+02:00 New cryptic species of the ‘revolutum’ group of Echinostoma (Digenea: Echinostomatidae) revealed by molecular and morphological data Georgieva, Simona Selbach, Christian Faltýnková, Anna Soldánová, Miroslava Sures, Bernd Skírnisson, Karl Kostadinova, Aneta 2013 http://dx.doi.org/10.1186/1756-3305-6-64 https://link.springer.com/content/pdf/10.1186/1756-3305-6-64.pdf en eng Springer Science and Business Media LLC Parasites & Vectors volume 6, issue 1 ISSN 1756-3305 Infectious Diseases Parasitology journal-article 2013 crspringernat https://doi.org/10.1186/1756-3305-6-64 2022-01-04T14:22:31Z Abstract Background The digenean species of Echinostoma (Echinostomatidae) with 37 collar spines that comprise the so-called ‘ revolutum ’ species complex, qualify as cryptic due to the interspecific homogeneity of characters used to differentiate species. Only five species were considered valid in the most recent revision of the group but recent molecular studies have demonstrated a higher diversity within the group. In a study of the digeneans parasitising molluscs in central and northern Europe we found that Radix auricularia , R. peregra and Stagnicola palustris were infected with larval stages of two cryptic species of the ‘ revolutum ’ complex, one resembling E. revolutum and one undescribed species, Echinostoma sp. IG. This paper provides morphological and molecular evidence for their delimitation. Methods Totals of 2,030 R. auricularia, 357 R. peregra and 577 S. palustris were collected in seven reservoirs of the River Ruhr catchment area in Germany and a total of 573 R. peregra was collected in five lakes in Iceland. Cercariae were examined and identified live and fixed in molecular grade ethanol for DNA isolation and in hot/cold 4% formaldehyde solution for obtaining measurements from fixed materials. Partial fragments of the mitochondrial gene nicotinamide adenine dinucleotide dehydrogenase subunit 1 ( nad 1) were amplified for 14 isolates. Results Detailed examination of cercarial morphology allowed us to differentiate the cercariae of the two Echinostoma spp. of the ‘ revolutum ’ species complex. A total of 14 partial nad 1 sequences was generated and aligned with selected published sequences for eight species of the ‘ revolutum ’ species complex. Both NJ and BI analyses resulted in consensus trees with similar topologies in which the isolates from Europe formed strongly supported reciprocally monophyletic lineages. The analyses also provided evidence that North American isolates identified as E. revolutum represent another cryptic species of the ‘ revolutum ’ species complex. Conclusion Our findings highlight the need for further analyses of patterns of interspecific variation based on molecular and morphological evidence to enhance the re-evaluation of the species and advance our understanding of the relationships within the ‘ revolutum ’ group of Echinostoma . Article in Journal/Newspaper Iceland Springer Nature (via Crossref) Parasites & Vectors 6 1 |
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Springer Nature (via Crossref) |
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Infectious Diseases Parasitology |
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Infectious Diseases Parasitology Georgieva, Simona Selbach, Christian Faltýnková, Anna Soldánová, Miroslava Sures, Bernd Skírnisson, Karl Kostadinova, Aneta New cryptic species of the ‘revolutum’ group of Echinostoma (Digenea: Echinostomatidae) revealed by molecular and morphological data |
topic_facet |
Infectious Diseases Parasitology |
description |
Abstract Background The digenean species of Echinostoma (Echinostomatidae) with 37 collar spines that comprise the so-called ‘ revolutum ’ species complex, qualify as cryptic due to the interspecific homogeneity of characters used to differentiate species. Only five species were considered valid in the most recent revision of the group but recent molecular studies have demonstrated a higher diversity within the group. In a study of the digeneans parasitising molluscs in central and northern Europe we found that Radix auricularia , R. peregra and Stagnicola palustris were infected with larval stages of two cryptic species of the ‘ revolutum ’ complex, one resembling E. revolutum and one undescribed species, Echinostoma sp. IG. This paper provides morphological and molecular evidence for their delimitation. Methods Totals of 2,030 R. auricularia, 357 R. peregra and 577 S. palustris were collected in seven reservoirs of the River Ruhr catchment area in Germany and a total of 573 R. peregra was collected in five lakes in Iceland. Cercariae were examined and identified live and fixed in molecular grade ethanol for DNA isolation and in hot/cold 4% formaldehyde solution for obtaining measurements from fixed materials. Partial fragments of the mitochondrial gene nicotinamide adenine dinucleotide dehydrogenase subunit 1 ( nad 1) were amplified for 14 isolates. Results Detailed examination of cercarial morphology allowed us to differentiate the cercariae of the two Echinostoma spp. of the ‘ revolutum ’ species complex. A total of 14 partial nad 1 sequences was generated and aligned with selected published sequences for eight species of the ‘ revolutum ’ species complex. Both NJ and BI analyses resulted in consensus trees with similar topologies in which the isolates from Europe formed strongly supported reciprocally monophyletic lineages. The analyses also provided evidence that North American isolates identified as E. revolutum represent another cryptic species of the ‘ revolutum ’ species complex. Conclusion Our findings highlight the need for further analyses of patterns of interspecific variation based on molecular and morphological evidence to enhance the re-evaluation of the species and advance our understanding of the relationships within the ‘ revolutum ’ group of Echinostoma . |
format |
Article in Journal/Newspaper |
author |
Georgieva, Simona Selbach, Christian Faltýnková, Anna Soldánová, Miroslava Sures, Bernd Skírnisson, Karl Kostadinova, Aneta |
author_facet |
Georgieva, Simona Selbach, Christian Faltýnková, Anna Soldánová, Miroslava Sures, Bernd Skírnisson, Karl Kostadinova, Aneta |
author_sort |
Georgieva, Simona |
title |
New cryptic species of the ‘revolutum’ group of Echinostoma (Digenea: Echinostomatidae) revealed by molecular and morphological data |
title_short |
New cryptic species of the ‘revolutum’ group of Echinostoma (Digenea: Echinostomatidae) revealed by molecular and morphological data |
title_full |
New cryptic species of the ‘revolutum’ group of Echinostoma (Digenea: Echinostomatidae) revealed by molecular and morphological data |
title_fullStr |
New cryptic species of the ‘revolutum’ group of Echinostoma (Digenea: Echinostomatidae) revealed by molecular and morphological data |
title_full_unstemmed |
New cryptic species of the ‘revolutum’ group of Echinostoma (Digenea: Echinostomatidae) revealed by molecular and morphological data |
title_sort |
new cryptic species of the ‘revolutum’ group of echinostoma (digenea: echinostomatidae) revealed by molecular and morphological data |
publisher |
Springer Science and Business Media LLC |
publishDate |
2013 |
url |
http://dx.doi.org/10.1186/1756-3305-6-64 https://link.springer.com/content/pdf/10.1186/1756-3305-6-64.pdf |
genre |
Iceland |
genre_facet |
Iceland |
op_source |
Parasites & Vectors volume 6, issue 1 ISSN 1756-3305 |
op_doi |
https://doi.org/10.1186/1756-3305-6-64 |
container_title |
Parasites & Vectors |
container_volume |
6 |
container_issue |
1 |
_version_ |
1766043453923262464 |