Transformation of metabolism with age and lifestyle in Antarctic seals: a case study of systems biology approach to cross-species microarray experiment
Abstract Background The metabolic transformation that changes Weddell seal pups born on land into aquatic animals is not only interesting for the study of general biology, but it also provides a model for the acquired and congenital muscle disorders which are associated with oxygen metabolism in ske...
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Online Access: | http://dx.doi.org/10.1186/1752-0509-4-133 https://link.springer.com/content/pdf/10.1186/1752-0509-4-133.pdf |
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crspringernat:10.1186/1752-0509-4-133 2023-05-15T14:12:38+02:00 Transformation of metabolism with age and lifestyle in Antarctic seals: a case study of systems biology approach to cross-species microarray experiment Ptitsyn, Andrey Schlater, Amber Kanatous, Shane 2010 http://dx.doi.org/10.1186/1752-0509-4-133 https://link.springer.com/content/pdf/10.1186/1752-0509-4-133.pdf en eng Springer Science and Business Media LLC BMC Systems Biology volume 4, issue 1 ISSN 1752-0509 Applied Mathematics Computer Science Applications Molecular Biology Modeling and Simulation Structural Biology journal-article 2010 crspringernat https://doi.org/10.1186/1752-0509-4-133 2022-01-04T07:13:31Z Abstract Background The metabolic transformation that changes Weddell seal pups born on land into aquatic animals is not only interesting for the study of general biology, but it also provides a model for the acquired and congenital muscle disorders which are associated with oxygen metabolism in skeletal muscle. However, the analysis of gene expression in seals is hampered by the lack of specific microarrays and the very limited annotation of known Weddell seal ( Leptonychotes weddellii ) genes. Results Muscle samples from newborn, juvenile, and adult Weddell seals were collected during an Antarctic expedition. Extracted RNA was hybridized on Affymetrix Human Expression chips. Preliminary studies showed a detectable signal from at least 7000 probe sets present in all samples and replicates. Relative expression levels for these genes was used for further analysis of the biological pathways implicated in the metabolism transformation which occurs in the transition from newborn, to juvenile, to adult seals. Cytoskeletal remodeling, WNT signaling, FAK signaling, hypoxia-induced HIF1 activation, and insulin regulation were identified as being among the most important biological pathways involved in transformation. Conclusion In spite of certain losses in specificity and sensitivity, the cross-species application of gene expression microarrays is capable of solving challenging puzzles in biology. A Systems Biology approach based on gene interaction patterns can compensate adequately for the lack of species-specific genomics information. Article in Journal/Newspaper Antarc* Antarctic Weddell Seal Weddell Seals Springer Nature (via Crossref) Antarctic Weddell BMC Systems Biology 4 1 133 |
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English |
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Applied Mathematics Computer Science Applications Molecular Biology Modeling and Simulation Structural Biology |
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Applied Mathematics Computer Science Applications Molecular Biology Modeling and Simulation Structural Biology Ptitsyn, Andrey Schlater, Amber Kanatous, Shane Transformation of metabolism with age and lifestyle in Antarctic seals: a case study of systems biology approach to cross-species microarray experiment |
topic_facet |
Applied Mathematics Computer Science Applications Molecular Biology Modeling and Simulation Structural Biology |
description |
Abstract Background The metabolic transformation that changes Weddell seal pups born on land into aquatic animals is not only interesting for the study of general biology, but it also provides a model for the acquired and congenital muscle disorders which are associated with oxygen metabolism in skeletal muscle. However, the analysis of gene expression in seals is hampered by the lack of specific microarrays and the very limited annotation of known Weddell seal ( Leptonychotes weddellii ) genes. Results Muscle samples from newborn, juvenile, and adult Weddell seals were collected during an Antarctic expedition. Extracted RNA was hybridized on Affymetrix Human Expression chips. Preliminary studies showed a detectable signal from at least 7000 probe sets present in all samples and replicates. Relative expression levels for these genes was used for further analysis of the biological pathways implicated in the metabolism transformation which occurs in the transition from newborn, to juvenile, to adult seals. Cytoskeletal remodeling, WNT signaling, FAK signaling, hypoxia-induced HIF1 activation, and insulin regulation were identified as being among the most important biological pathways involved in transformation. Conclusion In spite of certain losses in specificity and sensitivity, the cross-species application of gene expression microarrays is capable of solving challenging puzzles in biology. A Systems Biology approach based on gene interaction patterns can compensate adequately for the lack of species-specific genomics information. |
format |
Article in Journal/Newspaper |
author |
Ptitsyn, Andrey Schlater, Amber Kanatous, Shane |
author_facet |
Ptitsyn, Andrey Schlater, Amber Kanatous, Shane |
author_sort |
Ptitsyn, Andrey |
title |
Transformation of metabolism with age and lifestyle in Antarctic seals: a case study of systems biology approach to cross-species microarray experiment |
title_short |
Transformation of metabolism with age and lifestyle in Antarctic seals: a case study of systems biology approach to cross-species microarray experiment |
title_full |
Transformation of metabolism with age and lifestyle in Antarctic seals: a case study of systems biology approach to cross-species microarray experiment |
title_fullStr |
Transformation of metabolism with age and lifestyle in Antarctic seals: a case study of systems biology approach to cross-species microarray experiment |
title_full_unstemmed |
Transformation of metabolism with age and lifestyle in Antarctic seals: a case study of systems biology approach to cross-species microarray experiment |
title_sort |
transformation of metabolism with age and lifestyle in antarctic seals: a case study of systems biology approach to cross-species microarray experiment |
publisher |
Springer Science and Business Media LLC |
publishDate |
2010 |
url |
http://dx.doi.org/10.1186/1752-0509-4-133 https://link.springer.com/content/pdf/10.1186/1752-0509-4-133.pdf |
geographic |
Antarctic Weddell |
geographic_facet |
Antarctic Weddell |
genre |
Antarc* Antarctic Weddell Seal Weddell Seals |
genre_facet |
Antarc* Antarctic Weddell Seal Weddell Seals |
op_source |
BMC Systems Biology volume 4, issue 1 ISSN 1752-0509 |
op_doi |
https://doi.org/10.1186/1752-0509-4-133 |
container_title |
BMC Systems Biology |
container_volume |
4 |
container_issue |
1 |
container_start_page |
133 |
_version_ |
1766284970155835392 |