Polymorphisms within the Telomerase Reverse Transcriptase gene (TERT) in four breeds of dogs selected for difference in lifespan and cancer susceptibility

Abstract Background Enzymatic activity of Te lomerase R everse T ranscriptase (TERT) is important in maintaining the telomere length and has been implicated in cancer and aging related pathology. Since cancer susceptibility as well as longevity of dogs vary between breeds, this study involved sequen...

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Published in:BMC Veterinary Research
Main Authors: McAloney, Camille A, Silverstein, Kevin A T, Modiano, Jaime F, Bagchi, Anindya
Format: Article in Journal/Newspaper
Language:English
Published: Springer Science and Business Media LLC 2014
Subjects:
Online Access:http://dx.doi.org/10.1186/1746-6148-10-20
https://link.springer.com/content/pdf/10.1186/1746-6148-10-20.pdf
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spelling crspringernat:10.1186/1746-6148-10-20 2023-05-15T17:22:34+02:00 Polymorphisms within the Telomerase Reverse Transcriptase gene (TERT) in four breeds of dogs selected for difference in lifespan and cancer susceptibility McAloney, Camille A Silverstein, Kevin A T Modiano, Jaime F Bagchi, Anindya 2014 http://dx.doi.org/10.1186/1746-6148-10-20 https://link.springer.com/content/pdf/10.1186/1746-6148-10-20.pdf en eng Springer Science and Business Media LLC BMC Veterinary Research volume 10, issue 1 ISSN 1746-6148 General Veterinary General Medicine journal-article 2014 crspringernat https://doi.org/10.1186/1746-6148-10-20 2022-01-04T14:01:01Z Abstract Background Enzymatic activity of Te lomerase R everse T ranscriptase (TERT) is important in maintaining the telomere length and has been implicated in cancer and aging related pathology. Since cancer susceptibility as well as longevity of dogs vary between breeds, this study involved sequencing the entire TERT gene of Canis familiaris from DNA samples obtained from forty dogs, with ten dogs each of four breeds: Shih Tzu, Dachshund, Irish Wolfhound, and Newfoundland, each with different life expectancies and susceptibility to cancer. Results We compared the sequences of all forty individuals amongst one another and with the published sequence of canine TERT , and analyzed relationships between members of the same or different breeds. Two separate phylogenetic trees were generated and analyzed from these individuals. Polymorphisms were found most frequently in intronic regions of the gene, although exonic polymorphisms also were observed. In many locations genotypes were observed that were either homozygous for the reference sequence or heterozygous, but the variant homozygous genotype was not observed. Conclusions We propose that these homozygous variants are likely to have adverse effects in dogs. It was also found that the polymorphisms did not segregate by breed. Because the four breeds chosen come from geographically and physiologically distinct backgrounds, it can be inferred that the polymorphic diversification of TERT preceded breed derivation. Article in Journal/Newspaper Newfoundland Springer Nature (via Crossref) BMC Veterinary Research 10 1
institution Open Polar
collection Springer Nature (via Crossref)
op_collection_id crspringernat
language English
topic General Veterinary
General Medicine
spellingShingle General Veterinary
General Medicine
McAloney, Camille A
Silverstein, Kevin A T
Modiano, Jaime F
Bagchi, Anindya
Polymorphisms within the Telomerase Reverse Transcriptase gene (TERT) in four breeds of dogs selected for difference in lifespan and cancer susceptibility
topic_facet General Veterinary
General Medicine
description Abstract Background Enzymatic activity of Te lomerase R everse T ranscriptase (TERT) is important in maintaining the telomere length and has been implicated in cancer and aging related pathology. Since cancer susceptibility as well as longevity of dogs vary between breeds, this study involved sequencing the entire TERT gene of Canis familiaris from DNA samples obtained from forty dogs, with ten dogs each of four breeds: Shih Tzu, Dachshund, Irish Wolfhound, and Newfoundland, each with different life expectancies and susceptibility to cancer. Results We compared the sequences of all forty individuals amongst one another and with the published sequence of canine TERT , and analyzed relationships between members of the same or different breeds. Two separate phylogenetic trees were generated and analyzed from these individuals. Polymorphisms were found most frequently in intronic regions of the gene, although exonic polymorphisms also were observed. In many locations genotypes were observed that were either homozygous for the reference sequence or heterozygous, but the variant homozygous genotype was not observed. Conclusions We propose that these homozygous variants are likely to have adverse effects in dogs. It was also found that the polymorphisms did not segregate by breed. Because the four breeds chosen come from geographically and physiologically distinct backgrounds, it can be inferred that the polymorphic diversification of TERT preceded breed derivation.
format Article in Journal/Newspaper
author McAloney, Camille A
Silverstein, Kevin A T
Modiano, Jaime F
Bagchi, Anindya
author_facet McAloney, Camille A
Silverstein, Kevin A T
Modiano, Jaime F
Bagchi, Anindya
author_sort McAloney, Camille A
title Polymorphisms within the Telomerase Reverse Transcriptase gene (TERT) in four breeds of dogs selected for difference in lifespan and cancer susceptibility
title_short Polymorphisms within the Telomerase Reverse Transcriptase gene (TERT) in four breeds of dogs selected for difference in lifespan and cancer susceptibility
title_full Polymorphisms within the Telomerase Reverse Transcriptase gene (TERT) in four breeds of dogs selected for difference in lifespan and cancer susceptibility
title_fullStr Polymorphisms within the Telomerase Reverse Transcriptase gene (TERT) in four breeds of dogs selected for difference in lifespan and cancer susceptibility
title_full_unstemmed Polymorphisms within the Telomerase Reverse Transcriptase gene (TERT) in four breeds of dogs selected for difference in lifespan and cancer susceptibility
title_sort polymorphisms within the telomerase reverse transcriptase gene (tert) in four breeds of dogs selected for difference in lifespan and cancer susceptibility
publisher Springer Science and Business Media LLC
publishDate 2014
url http://dx.doi.org/10.1186/1746-6148-10-20
https://link.springer.com/content/pdf/10.1186/1746-6148-10-20.pdf
genre Newfoundland
genre_facet Newfoundland
op_source BMC Veterinary Research
volume 10, issue 1
ISSN 1746-6148
op_doi https://doi.org/10.1186/1746-6148-10-20
container_title BMC Veterinary Research
container_volume 10
container_issue 1
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