Differentiation of canine distemper virus isolates in fur animals from various vaccine strains by reverse transcription-polymerase chain reaction-restriction fragment length polymorphism according to phylogenetic relations in china
Abstract In order to effectively identify the vaccine and field strains of Canine distemper virus (CDV), a new differential diagnostic test has been developed based on reverse transcription-polymerase chain reaction (RT-PCR) and restriction fragment length polymorphism (RFLP). We selected an 829 bp...
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Online Access: | http://dx.doi.org/10.1186/1743-422x-8-85 https://link.springer.com/content/pdf/10.1186/1743-422X-8-85.pdf |
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crspringernat:10.1186/1743-422x-8-85 2023-05-15T15:07:56+02:00 Differentiation of canine distemper virus isolates in fur animals from various vaccine strains by reverse transcription-polymerase chain reaction-restriction fragment length polymorphism according to phylogenetic relations in china Wang, Fengxue Yan, Xijun Chai, Xiuli Zhang, Hailing Zhao, Jianjun Wen, Yongjun Wu, Wei 2011 http://dx.doi.org/10.1186/1743-422x-8-85 https://link.springer.com/content/pdf/10.1186/1743-422X-8-85.pdf en eng Springer Science and Business Media LLC Virology Journal volume 8, issue 1 ISSN 1743-422X Infectious Diseases Virology journal-article 2011 crspringernat https://doi.org/10.1186/1743-422x-8-85 2022-01-04T07:39:14Z Abstract In order to effectively identify the vaccine and field strains of Canine distemper virus (CDV), a new differential diagnostic test has been developed based on reverse transcription-polymerase chain reaction (RT-PCR) and restriction fragment length polymorphism (RFLP). We selected an 829 bp fragment of the nucleoprotein (N) gene of CDV. By RFLP analysis using Bam HI, field isolates were distinguishable from the vaccine strains. Two fragments were obtained from the vaccine strains by RT-PCR-RFLP analysis while three were observed in the field strains. An 829 nucleotide region of the CDV N gene was analyzed in 19 CDV field strains isolated from minks, raccoon dogs and foxes in China between 2005 and 2007. The results suggest this method is precise, accurate and efficient. It was also determined that three different genotypes exist in CDV field strains in fur animal herds of the north of China, most of which belong to Asian type. Mutated field strains, JSY06-R1, JSY06-R2 and JDH07-F1 also exist in Northern China, but are most closely related to the standard virulent strain A75/17, designated in Arctic and America-2 genetype in the present study, respectively. Article in Journal/Newspaper Arctic Springer Nature (via Crossref) Arctic Virology Journal 8 1 |
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Springer Nature (via Crossref) |
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Infectious Diseases Virology |
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Infectious Diseases Virology Wang, Fengxue Yan, Xijun Chai, Xiuli Zhang, Hailing Zhao, Jianjun Wen, Yongjun Wu, Wei Differentiation of canine distemper virus isolates in fur animals from various vaccine strains by reverse transcription-polymerase chain reaction-restriction fragment length polymorphism according to phylogenetic relations in china |
topic_facet |
Infectious Diseases Virology |
description |
Abstract In order to effectively identify the vaccine and field strains of Canine distemper virus (CDV), a new differential diagnostic test has been developed based on reverse transcription-polymerase chain reaction (RT-PCR) and restriction fragment length polymorphism (RFLP). We selected an 829 bp fragment of the nucleoprotein (N) gene of CDV. By RFLP analysis using Bam HI, field isolates were distinguishable from the vaccine strains. Two fragments were obtained from the vaccine strains by RT-PCR-RFLP analysis while three were observed in the field strains. An 829 nucleotide region of the CDV N gene was analyzed in 19 CDV field strains isolated from minks, raccoon dogs and foxes in China between 2005 and 2007. The results suggest this method is precise, accurate and efficient. It was also determined that three different genotypes exist in CDV field strains in fur animal herds of the north of China, most of which belong to Asian type. Mutated field strains, JSY06-R1, JSY06-R2 and JDH07-F1 also exist in Northern China, but are most closely related to the standard virulent strain A75/17, designated in Arctic and America-2 genetype in the present study, respectively. |
format |
Article in Journal/Newspaper |
author |
Wang, Fengxue Yan, Xijun Chai, Xiuli Zhang, Hailing Zhao, Jianjun Wen, Yongjun Wu, Wei |
author_facet |
Wang, Fengxue Yan, Xijun Chai, Xiuli Zhang, Hailing Zhao, Jianjun Wen, Yongjun Wu, Wei |
author_sort |
Wang, Fengxue |
title |
Differentiation of canine distemper virus isolates in fur animals from various vaccine strains by reverse transcription-polymerase chain reaction-restriction fragment length polymorphism according to phylogenetic relations in china |
title_short |
Differentiation of canine distemper virus isolates in fur animals from various vaccine strains by reverse transcription-polymerase chain reaction-restriction fragment length polymorphism according to phylogenetic relations in china |
title_full |
Differentiation of canine distemper virus isolates in fur animals from various vaccine strains by reverse transcription-polymerase chain reaction-restriction fragment length polymorphism according to phylogenetic relations in china |
title_fullStr |
Differentiation of canine distemper virus isolates in fur animals from various vaccine strains by reverse transcription-polymerase chain reaction-restriction fragment length polymorphism according to phylogenetic relations in china |
title_full_unstemmed |
Differentiation of canine distemper virus isolates in fur animals from various vaccine strains by reverse transcription-polymerase chain reaction-restriction fragment length polymorphism according to phylogenetic relations in china |
title_sort |
differentiation of canine distemper virus isolates in fur animals from various vaccine strains by reverse transcription-polymerase chain reaction-restriction fragment length polymorphism according to phylogenetic relations in china |
publisher |
Springer Science and Business Media LLC |
publishDate |
2011 |
url |
http://dx.doi.org/10.1186/1743-422x-8-85 https://link.springer.com/content/pdf/10.1186/1743-422X-8-85.pdf |
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Arctic |
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Arctic |
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Arctic |
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Arctic |
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Virology Journal volume 8, issue 1 ISSN 1743-422X |
op_doi |
https://doi.org/10.1186/1743-422x-8-85 |
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Virology Journal |
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8 |
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1 |
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1766339357143203840 |