Characterisation of parapoxviruses isolated from Norwegian semi-domesticated reindeer (Rangifer tarandus tarandus)
Abstract Background Two outbreaks of the disease contagious ecthyma were reported in 1999 and 2000 in Norwegian semi-domesticated reindeer ( Rangifer tarandus tarandus ). Contagious ecthyma is an epidermal disease of sheep and goats worldwide, which is caused by the zoonotic parapoxvirus orf virus....
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2005
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Online Access: | http://dx.doi.org/10.1186/1743-422x-2-79 https://link.springer.com/content/pdf/10.1186/1743-422X-2-79.pdf |
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crspringernat:10.1186/1743-422x-2-79 2023-05-15T18:04:16+02:00 Characterisation of parapoxviruses isolated from Norwegian semi-domesticated reindeer (Rangifer tarandus tarandus) Klein, Joern Tryland, Morten 2005 http://dx.doi.org/10.1186/1743-422x-2-79 https://link.springer.com/content/pdf/10.1186/1743-422X-2-79.pdf en eng Springer Science and Business Media LLC Virology Journal volume 2, issue 1 ISSN 1743-422X Infectious Diseases Virology journal-article 2005 crspringernat https://doi.org/10.1186/1743-422x-2-79 2022-01-04T16:28:37Z Abstract Background Two outbreaks of the disease contagious ecthyma were reported in 1999 and 2000 in Norwegian semi-domesticated reindeer ( Rangifer tarandus tarandus ). Contagious ecthyma is an epidermal disease of sheep and goats worldwide, which is caused by the zoonotic parapoxvirus orf virus. Characterisation of clinical samples from the two outbreaks in semi-domesticated reindeer in Norway by electron microscopy and PCR (B2L) revealed typical parapoxvirus particles and partial gene sequences corresponding to parapoxvirus, respectively. If contagious ecthyma in reindeer is caused by orf virus, the virus may be transferred from sheep and goats, via people, equipment and common use of pastures and corrals, to reindeer. Another possibility is that contagious ecthyma in reindeer is caused by a hitherto unclassified member of the parapoxvirus genus that circulates among reindeer herds and remains endemic in Norway. Results Genomic comparisons of one standard orf strain (orf NZ2) and the reindeer isolates, employing restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA (RAPD) analysis, demonstrated high similarity between the reindeer viruses and known orf virus strains. Partial DNA sequences of two different viral genes were determined for the different isolates and compared with corresponding parapoxvirus genebank sequences. The comparison/alignment and construction of phylogenetic trees also point to an affiliation of the reindeer viruses to the species orf virus. Conclusion The results of this work imply that the parapoxvirus causing contagious ecthyma in Norwegian semi-domesticated reindeer belongs to the species orf virus and that the orf virus crosses the host species barrier from sheep and goat to semi-domesticated reindeer. Article in Journal/Newspaper Rangifer tarandus Springer Nature (via Crossref) Norway Virology Journal 2 1 |
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Open Polar |
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Springer Nature (via Crossref) |
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English |
topic |
Infectious Diseases Virology |
spellingShingle |
Infectious Diseases Virology Klein, Joern Tryland, Morten Characterisation of parapoxviruses isolated from Norwegian semi-domesticated reindeer (Rangifer tarandus tarandus) |
topic_facet |
Infectious Diseases Virology |
description |
Abstract Background Two outbreaks of the disease contagious ecthyma were reported in 1999 and 2000 in Norwegian semi-domesticated reindeer ( Rangifer tarandus tarandus ). Contagious ecthyma is an epidermal disease of sheep and goats worldwide, which is caused by the zoonotic parapoxvirus orf virus. Characterisation of clinical samples from the two outbreaks in semi-domesticated reindeer in Norway by electron microscopy and PCR (B2L) revealed typical parapoxvirus particles and partial gene sequences corresponding to parapoxvirus, respectively. If contagious ecthyma in reindeer is caused by orf virus, the virus may be transferred from sheep and goats, via people, equipment and common use of pastures and corrals, to reindeer. Another possibility is that contagious ecthyma in reindeer is caused by a hitherto unclassified member of the parapoxvirus genus that circulates among reindeer herds and remains endemic in Norway. Results Genomic comparisons of one standard orf strain (orf NZ2) and the reindeer isolates, employing restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA (RAPD) analysis, demonstrated high similarity between the reindeer viruses and known orf virus strains. Partial DNA sequences of two different viral genes were determined for the different isolates and compared with corresponding parapoxvirus genebank sequences. The comparison/alignment and construction of phylogenetic trees also point to an affiliation of the reindeer viruses to the species orf virus. Conclusion The results of this work imply that the parapoxvirus causing contagious ecthyma in Norwegian semi-domesticated reindeer belongs to the species orf virus and that the orf virus crosses the host species barrier from sheep and goat to semi-domesticated reindeer. |
format |
Article in Journal/Newspaper |
author |
Klein, Joern Tryland, Morten |
author_facet |
Klein, Joern Tryland, Morten |
author_sort |
Klein, Joern |
title |
Characterisation of parapoxviruses isolated from Norwegian semi-domesticated reindeer (Rangifer tarandus tarandus) |
title_short |
Characterisation of parapoxviruses isolated from Norwegian semi-domesticated reindeer (Rangifer tarandus tarandus) |
title_full |
Characterisation of parapoxviruses isolated from Norwegian semi-domesticated reindeer (Rangifer tarandus tarandus) |
title_fullStr |
Characterisation of parapoxviruses isolated from Norwegian semi-domesticated reindeer (Rangifer tarandus tarandus) |
title_full_unstemmed |
Characterisation of parapoxviruses isolated from Norwegian semi-domesticated reindeer (Rangifer tarandus tarandus) |
title_sort |
characterisation of parapoxviruses isolated from norwegian semi-domesticated reindeer (rangifer tarandus tarandus) |
publisher |
Springer Science and Business Media LLC |
publishDate |
2005 |
url |
http://dx.doi.org/10.1186/1743-422x-2-79 https://link.springer.com/content/pdf/10.1186/1743-422X-2-79.pdf |
geographic |
Norway |
geographic_facet |
Norway |
genre |
Rangifer tarandus |
genre_facet |
Rangifer tarandus |
op_source |
Virology Journal volume 2, issue 1 ISSN 1743-422X |
op_doi |
https://doi.org/10.1186/1743-422x-2-79 |
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Virology Journal |
container_volume |
2 |
container_issue |
1 |
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1766175597576323072 |