Constitutive expression of Atlantic salmon Mx1 protein in CHSE-214 cells confers resistance to Infectious Salmon Anaemia virus
Abstract Infectious salmon anaemia (ISA) is a highly fatal viral disease affecting marine-farmed Atlantic salmon which is caused by ISA virus (ISAV), a fish orthomyxovirus that has recently been assigned to the new genus Isavirus within the family Orthomyxoviridae . Mx proteins are among the interfe...
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2005
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Online Access: | http://dx.doi.org/10.1186/1743-422x-2-75 https://link.springer.com/content/pdf/10.1186/1743-422X-2-75.pdf |
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crspringernat:10.1186/1743-422x-2-75 2023-05-15T15:30:38+02:00 Constitutive expression of Atlantic salmon Mx1 protein in CHSE-214 cells confers resistance to Infectious Salmon Anaemia virus Kibenge, Molly JT Munir, Khalid Kibenge, Frederick SB 2005 http://dx.doi.org/10.1186/1743-422x-2-75 https://link.springer.com/content/pdf/10.1186/1743-422X-2-75.pdf en eng Springer Science and Business Media LLC Virology Journal volume 2, issue 1 ISSN 1743-422X Infectious Diseases Virology journal-article 2005 crspringernat https://doi.org/10.1186/1743-422x-2-75 2021-11-02T17:19:53Z Abstract Infectious salmon anaemia (ISA) is a highly fatal viral disease affecting marine-farmed Atlantic salmon which is caused by ISA virus (ISAV), a fish orthomyxovirus that has recently been assigned to the new genus Isavirus within the family Orthomyxoviridae . Mx proteins are among the interferon (IFN)-induced proteins responsible for the development of an antiviral state in vertebrate cells. We used real-time reverse transcription-polymerase chain reaction (RT-PCR) and Chinook salmon embryo (CHSE-214) cells constitutively expressing Atlantic salmon Mx1 protein (ASMx1) to examine the antiviral properties of ASMx1 against two ISAV strains, NBISA01 and HKS-36, having phenotypically different growth properties (cytopathic vs non-cytopathic) in the CHSE-214 cell line. We present evidence that ISAV is sensitive to ASMx1. CHSE-214 cells constitutively expressing ASMx1 showed increased resistance to infection with the cytopathic ISAV strain NBISA01, manifested as delayed development of cytopathic effects (CPE) and significant reduction in the severity of CPE, as well as a 10-fold reduction in virus yield. However, by real-time RT-PCR we observed no significant difference in the mean threshold cycle (Ct) values of ISAV RNA levels, suggesting that the ASMx1 activity on ISAV occurs at the post-transcription steps of virus replication, possibly in the cytoplasm. Article in Journal/Newspaper Atlantic salmon Springer Nature (via Crossref) Virology Journal 2 1 |
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Springer Nature (via Crossref) |
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English |
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Infectious Diseases Virology |
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Infectious Diseases Virology Kibenge, Molly JT Munir, Khalid Kibenge, Frederick SB Constitutive expression of Atlantic salmon Mx1 protein in CHSE-214 cells confers resistance to Infectious Salmon Anaemia virus |
topic_facet |
Infectious Diseases Virology |
description |
Abstract Infectious salmon anaemia (ISA) is a highly fatal viral disease affecting marine-farmed Atlantic salmon which is caused by ISA virus (ISAV), a fish orthomyxovirus that has recently been assigned to the new genus Isavirus within the family Orthomyxoviridae . Mx proteins are among the interferon (IFN)-induced proteins responsible for the development of an antiviral state in vertebrate cells. We used real-time reverse transcription-polymerase chain reaction (RT-PCR) and Chinook salmon embryo (CHSE-214) cells constitutively expressing Atlantic salmon Mx1 protein (ASMx1) to examine the antiviral properties of ASMx1 against two ISAV strains, NBISA01 and HKS-36, having phenotypically different growth properties (cytopathic vs non-cytopathic) in the CHSE-214 cell line. We present evidence that ISAV is sensitive to ASMx1. CHSE-214 cells constitutively expressing ASMx1 showed increased resistance to infection with the cytopathic ISAV strain NBISA01, manifested as delayed development of cytopathic effects (CPE) and significant reduction in the severity of CPE, as well as a 10-fold reduction in virus yield. However, by real-time RT-PCR we observed no significant difference in the mean threshold cycle (Ct) values of ISAV RNA levels, suggesting that the ASMx1 activity on ISAV occurs at the post-transcription steps of virus replication, possibly in the cytoplasm. |
format |
Article in Journal/Newspaper |
author |
Kibenge, Molly JT Munir, Khalid Kibenge, Frederick SB |
author_facet |
Kibenge, Molly JT Munir, Khalid Kibenge, Frederick SB |
author_sort |
Kibenge, Molly JT |
title |
Constitutive expression of Atlantic salmon Mx1 protein in CHSE-214 cells confers resistance to Infectious Salmon Anaemia virus |
title_short |
Constitutive expression of Atlantic salmon Mx1 protein in CHSE-214 cells confers resistance to Infectious Salmon Anaemia virus |
title_full |
Constitutive expression of Atlantic salmon Mx1 protein in CHSE-214 cells confers resistance to Infectious Salmon Anaemia virus |
title_fullStr |
Constitutive expression of Atlantic salmon Mx1 protein in CHSE-214 cells confers resistance to Infectious Salmon Anaemia virus |
title_full_unstemmed |
Constitutive expression of Atlantic salmon Mx1 protein in CHSE-214 cells confers resistance to Infectious Salmon Anaemia virus |
title_sort |
constitutive expression of atlantic salmon mx1 protein in chse-214 cells confers resistance to infectious salmon anaemia virus |
publisher |
Springer Science and Business Media LLC |
publishDate |
2005 |
url |
http://dx.doi.org/10.1186/1743-422x-2-75 https://link.springer.com/content/pdf/10.1186/1743-422X-2-75.pdf |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
Virology Journal volume 2, issue 1 ISSN 1743-422X |
op_doi |
https://doi.org/10.1186/1743-422x-2-75 |
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Virology Journal |
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2 |
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1 |
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1766361077194424320 |