Biochemical characterization of the Arctic char (Salvelinus alpinus) ovarian progestin membrane receptor
Abstract Membrane progestin receptors are involved in oocyte maturation in teleosts. However, the maturation-inducing steroid (MIS) does not appear to be conserved among species and several progestins may fulfill this function. So far, complete biochemical characterization has only been performed on...
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Online Access: | http://dx.doi.org/10.1186/1477-7827-3-64 https://link.springer.com/content/pdf/10.1186/1477-7827-3-64.pdf |
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crspringernat:10.1186/1477-7827-3-64 2023-05-15T14:56:57+02:00 Biochemical characterization of the Arctic char (Salvelinus alpinus) ovarian progestin membrane receptor Berg, A Håkan Thomas, Peter Olsson, Per-Erik 2005 http://dx.doi.org/10.1186/1477-7827-3-64 https://link.springer.com/content/pdf/10.1186/1477-7827-3-64.pdf en eng Springer Science and Business Media LLC Reproductive Biology and Endocrinology volume 3, issue 1 ISSN 1477-7827 Developmental Biology Endocrinology Reproductive Medicine Obstetrics and Gynecology journal-article 2005 crspringernat https://doi.org/10.1186/1477-7827-3-64 2022-01-04T16:24:00Z Abstract Membrane progestin receptors are involved in oocyte maturation in teleosts. However, the maturation-inducing steroid (MIS) does not appear to be conserved among species and several progestins may fulfill this function. So far, complete biochemical characterization has only been performed on a few species. In the present study we have characterized the membrane progestin receptor in Arctic char (Salvelinus alpinu s ) and show that the 17,20beta-dihydroxy-4-pregnen-3-one (17,20beta-P) receptor also binds several xenobiotics, thus rendering oocyte maturation sensitive to environmental pollutants. We identified a single class of high affinity (K d , 13.8 ± 1.1 nM), low capacity (B max , 1.6 ± 0.6 pmol/g ovary) binding sites by saturation and Scatchard analyses. Receptor binding displayed rapid association and dissociation kinetics typical of steroid membrane receptors, with t 1/2 s of less than 1 minute. The 17,20beta-P binding also displayed tissue specificity with high, saturable, and specific 17,20beta-P binding detected in ovaries, heart and gills while no specific binding was observed in muscle, brain or liver. Changes in 17,20beta-P binding during oocyte maturation were consistent with its identity as the oocyte MIS membrane receptor. Incubation of fully-grown ovarian follicles with gonadotropin induced oocyte maturation, which was accompanied by a five-fold increase in 17,20beta-P receptor binding. In addition, competition studies with a variety of steroids revealed that receptor binding is highly specific for 17,20beta-P, the likely maturation-inducing steroid (MIS) in Arctic char. The relative-binding affinities of all the other progestogens and steroids tested were less than 5% of that of 17,20beta-P for the receptor. Several ortho, para derivatives of DDT also showed weak binding affinity for the 17,20beta-P receptor supporting the hypothesis that xenobiotics may bind steroid receptors on the oocyte's surface and might thereby interfere with oocyte growth and maturation. Article in Journal/Newspaper Arctic Salvelinus alpinus Springer Nature (via Crossref) Arctic Reproductive Biology and Endocrinology 3 1 |
institution |
Open Polar |
collection |
Springer Nature (via Crossref) |
op_collection_id |
crspringernat |
language |
English |
topic |
Developmental Biology Endocrinology Reproductive Medicine Obstetrics and Gynecology |
spellingShingle |
Developmental Biology Endocrinology Reproductive Medicine Obstetrics and Gynecology Berg, A Håkan Thomas, Peter Olsson, Per-Erik Biochemical characterization of the Arctic char (Salvelinus alpinus) ovarian progestin membrane receptor |
topic_facet |
Developmental Biology Endocrinology Reproductive Medicine Obstetrics and Gynecology |
description |
Abstract Membrane progestin receptors are involved in oocyte maturation in teleosts. However, the maturation-inducing steroid (MIS) does not appear to be conserved among species and several progestins may fulfill this function. So far, complete biochemical characterization has only been performed on a few species. In the present study we have characterized the membrane progestin receptor in Arctic char (Salvelinus alpinu s ) and show that the 17,20beta-dihydroxy-4-pregnen-3-one (17,20beta-P) receptor also binds several xenobiotics, thus rendering oocyte maturation sensitive to environmental pollutants. We identified a single class of high affinity (K d , 13.8 ± 1.1 nM), low capacity (B max , 1.6 ± 0.6 pmol/g ovary) binding sites by saturation and Scatchard analyses. Receptor binding displayed rapid association and dissociation kinetics typical of steroid membrane receptors, with t 1/2 s of less than 1 minute. The 17,20beta-P binding also displayed tissue specificity with high, saturable, and specific 17,20beta-P binding detected in ovaries, heart and gills while no specific binding was observed in muscle, brain or liver. Changes in 17,20beta-P binding during oocyte maturation were consistent with its identity as the oocyte MIS membrane receptor. Incubation of fully-grown ovarian follicles with gonadotropin induced oocyte maturation, which was accompanied by a five-fold increase in 17,20beta-P receptor binding. In addition, competition studies with a variety of steroids revealed that receptor binding is highly specific for 17,20beta-P, the likely maturation-inducing steroid (MIS) in Arctic char. The relative-binding affinities of all the other progestogens and steroids tested were less than 5% of that of 17,20beta-P for the receptor. Several ortho, para derivatives of DDT also showed weak binding affinity for the 17,20beta-P receptor supporting the hypothesis that xenobiotics may bind steroid receptors on the oocyte's surface and might thereby interfere with oocyte growth and maturation. |
format |
Article in Journal/Newspaper |
author |
Berg, A Håkan Thomas, Peter Olsson, Per-Erik |
author_facet |
Berg, A Håkan Thomas, Peter Olsson, Per-Erik |
author_sort |
Berg, A Håkan |
title |
Biochemical characterization of the Arctic char (Salvelinus alpinus) ovarian progestin membrane receptor |
title_short |
Biochemical characterization of the Arctic char (Salvelinus alpinus) ovarian progestin membrane receptor |
title_full |
Biochemical characterization of the Arctic char (Salvelinus alpinus) ovarian progestin membrane receptor |
title_fullStr |
Biochemical characterization of the Arctic char (Salvelinus alpinus) ovarian progestin membrane receptor |
title_full_unstemmed |
Biochemical characterization of the Arctic char (Salvelinus alpinus) ovarian progestin membrane receptor |
title_sort |
biochemical characterization of the arctic char (salvelinus alpinus) ovarian progestin membrane receptor |
publisher |
Springer Science and Business Media LLC |
publishDate |
2005 |
url |
http://dx.doi.org/10.1186/1477-7827-3-64 https://link.springer.com/content/pdf/10.1186/1477-7827-3-64.pdf |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Salvelinus alpinus |
genre_facet |
Arctic Salvelinus alpinus |
op_source |
Reproductive Biology and Endocrinology volume 3, issue 1 ISSN 1477-7827 |
op_doi |
https://doi.org/10.1186/1477-7827-3-64 |
container_title |
Reproductive Biology and Endocrinology |
container_volume |
3 |
container_issue |
1 |
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1766329013663432704 |