Polygonum viviparum L. induces vasorelaxation in the rat thoracic aorta via activation of nitric oxide synthase in endothelial cells
Abstract Background In the past several decades, Polygonum viviparum L. (PV) was reported to have antibacterial, antiulcer, antioxidant, antitumor, anti-inflammatory, and antiarthritic properties. The anti-inflammatory pathway was recently elucidated through cytosolic nuclear factor E2-related facto...
Published in: | BMC Complementary and Alternative Medicine |
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Springer Science and Business Media LLC
2014
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Online Access: | http://dx.doi.org/10.1186/1472-6882-14-150 http://link.springer.com/content/pdf/10.1186/1472-6882-14-150.pdf http://link.springer.com/article/10.1186/1472-6882-14-150/fulltext.html http://link.springer.com/content/pdf/10.1186/1472-6882-14-150 https://link.springer.com/content/pdf/10.1186/1472-6882-14-150.pdf |
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crspringernat:10.1186/1472-6882-14-150 2023-05-15T18:03:01+02:00 Polygonum viviparum L. induces vasorelaxation in the rat thoracic aorta via activation of nitric oxide synthase in endothelial cells Chang, Ming-Long Chang, Jung-Su Yu, Wen-Yu Cheah, Khoot-Peng Li, Joe-Sharg Cheng, Hui-Wen Hu, Chien-Ming 2014 http://dx.doi.org/10.1186/1472-6882-14-150 http://link.springer.com/content/pdf/10.1186/1472-6882-14-150.pdf http://link.springer.com/article/10.1186/1472-6882-14-150/fulltext.html http://link.springer.com/content/pdf/10.1186/1472-6882-14-150 https://link.springer.com/content/pdf/10.1186/1472-6882-14-150.pdf en eng Springer Science and Business Media LLC http://www.springer.com/tdm BMC Complementary and Alternative Medicine volume 14, issue 1 ISSN 1472-6882 Complementary and alternative medicine General Medicine journal-article 2014 crspringernat https://doi.org/10.1186/1472-6882-14-150 2022-01-04T08:04:00Z Abstract Background In the past several decades, Polygonum viviparum L. (PV) was reported to have antibacterial, antiulcer, antioxidant, antitumor, anti-inflammatory, and antiarthritic properties. The anti-inflammatory pathway was recently elucidated through cytosolic nuclear factor E2-related factor 2 (Nrf2) activation and heme oxygenase (HO)-1 protein expression. PV is a perennial herb and widely distributed in high-elevation mountain regions, such as the Tibetan Plateau. In Tibetan traditional medicine, PV is usually used to boost the blood circulation to dissipate blood stasis. Therefore, this study focused on how PV improves the vascular circulation and acts on vascular tissues. Methods In this study, we isolated aortas from Sprague-Dawley rats (male, weight about 250 ~ 350 g), and detected the effects of PV on phenylephrine (PE)-induced contraction and cyclic guanosine 3′,5′-monophosphate (cGMP) formation using aortic rings. In addition, human umbilical vein endothelial cells (HUVECs) were used to exam nitric oxygen (NO) synthase (NOS) activity by directly measuring NO production in the culture medium. Endothelial (e) NOS phosphorylation, and cytosolic Nrf2 and HO-1 expressions were measured using a Western blot analysis. Results PV dose-dependently relaxed PE-induced contractions in endothelial-intact but not -denuded aorta. The concentration to produce 50% relaxation was 22.04 ± 1.77 μg/ml. PV-induced vasorelaxation was markedly blocked by pretreatment with N G -nitro- L -arginine methyl ester (L-NAME), an NOS inhibitor, methylene blue (MB), a guanylyl cyclase inhibitor, and hemoglobin, an NO scavenger. PV increased cGMP formation; however, this effect was also suppressed by co-pretreatment with l -NAME, MB, hemoglobin, and Ca 2+ -free medium. In HUVECs, PV increased NO formation, which was greatly attenuated by NOS inhibitors (L-NAME and L-NMMA) and by removing extracellular Ca 2+ and chelating intracellular Ca 2+ with BAPTA-AM. In addition, PV promoted eNOS phosphorylation, Nrf2 degradation, and HO-1 protein expression according to a Western blot analysis. Conclusions The results suggest that PV possesses vasorelaxing action in an endothelium-dependent manner and works through activating Ca 2+ /calmodulin- dependent NO synthesis; when NO is released and then transferred to smooth muscle cells, NO activates guanylyl cyclase and increases cGMP formation, ultimately resulting in vasorelaxation. Thus, PV can be considered for application as a potential therapeutic approach for vascular-associated disorders. Article in Journal/Newspaper Polygonum viviparum Springer Nature (via Crossref) BMC Complementary and Alternative Medicine 14 1 |
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Springer Nature (via Crossref) |
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topic |
Complementary and alternative medicine General Medicine |
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Complementary and alternative medicine General Medicine Chang, Ming-Long Chang, Jung-Su Yu, Wen-Yu Cheah, Khoot-Peng Li, Joe-Sharg Cheng, Hui-Wen Hu, Chien-Ming Polygonum viviparum L. induces vasorelaxation in the rat thoracic aorta via activation of nitric oxide synthase in endothelial cells |
topic_facet |
Complementary and alternative medicine General Medicine |
description |
Abstract Background In the past several decades, Polygonum viviparum L. (PV) was reported to have antibacterial, antiulcer, antioxidant, antitumor, anti-inflammatory, and antiarthritic properties. The anti-inflammatory pathway was recently elucidated through cytosolic nuclear factor E2-related factor 2 (Nrf2) activation and heme oxygenase (HO)-1 protein expression. PV is a perennial herb and widely distributed in high-elevation mountain regions, such as the Tibetan Plateau. In Tibetan traditional medicine, PV is usually used to boost the blood circulation to dissipate blood stasis. Therefore, this study focused on how PV improves the vascular circulation and acts on vascular tissues. Methods In this study, we isolated aortas from Sprague-Dawley rats (male, weight about 250 ~ 350 g), and detected the effects of PV on phenylephrine (PE)-induced contraction and cyclic guanosine 3′,5′-monophosphate (cGMP) formation using aortic rings. In addition, human umbilical vein endothelial cells (HUVECs) were used to exam nitric oxygen (NO) synthase (NOS) activity by directly measuring NO production in the culture medium. Endothelial (e) NOS phosphorylation, and cytosolic Nrf2 and HO-1 expressions were measured using a Western blot analysis. Results PV dose-dependently relaxed PE-induced contractions in endothelial-intact but not -denuded aorta. The concentration to produce 50% relaxation was 22.04 ± 1.77 μg/ml. PV-induced vasorelaxation was markedly blocked by pretreatment with N G -nitro- L -arginine methyl ester (L-NAME), an NOS inhibitor, methylene blue (MB), a guanylyl cyclase inhibitor, and hemoglobin, an NO scavenger. PV increased cGMP formation; however, this effect was also suppressed by co-pretreatment with l -NAME, MB, hemoglobin, and Ca 2+ -free medium. In HUVECs, PV increased NO formation, which was greatly attenuated by NOS inhibitors (L-NAME and L-NMMA) and by removing extracellular Ca 2+ and chelating intracellular Ca 2+ with BAPTA-AM. In addition, PV promoted eNOS phosphorylation, Nrf2 degradation, and HO-1 protein expression according to a Western blot analysis. Conclusions The results suggest that PV possesses vasorelaxing action in an endothelium-dependent manner and works through activating Ca 2+ /calmodulin- dependent NO synthesis; when NO is released and then transferred to smooth muscle cells, NO activates guanylyl cyclase and increases cGMP formation, ultimately resulting in vasorelaxation. Thus, PV can be considered for application as a potential therapeutic approach for vascular-associated disorders. |
format |
Article in Journal/Newspaper |
author |
Chang, Ming-Long Chang, Jung-Su Yu, Wen-Yu Cheah, Khoot-Peng Li, Joe-Sharg Cheng, Hui-Wen Hu, Chien-Ming |
author_facet |
Chang, Ming-Long Chang, Jung-Su Yu, Wen-Yu Cheah, Khoot-Peng Li, Joe-Sharg Cheng, Hui-Wen Hu, Chien-Ming |
author_sort |
Chang, Ming-Long |
title |
Polygonum viviparum L. induces vasorelaxation in the rat thoracic aorta via activation of nitric oxide synthase in endothelial cells |
title_short |
Polygonum viviparum L. induces vasorelaxation in the rat thoracic aorta via activation of nitric oxide synthase in endothelial cells |
title_full |
Polygonum viviparum L. induces vasorelaxation in the rat thoracic aorta via activation of nitric oxide synthase in endothelial cells |
title_fullStr |
Polygonum viviparum L. induces vasorelaxation in the rat thoracic aorta via activation of nitric oxide synthase in endothelial cells |
title_full_unstemmed |
Polygonum viviparum L. induces vasorelaxation in the rat thoracic aorta via activation of nitric oxide synthase in endothelial cells |
title_sort |
polygonum viviparum l. induces vasorelaxation in the rat thoracic aorta via activation of nitric oxide synthase in endothelial cells |
publisher |
Springer Science and Business Media LLC |
publishDate |
2014 |
url |
http://dx.doi.org/10.1186/1472-6882-14-150 http://link.springer.com/content/pdf/10.1186/1472-6882-14-150.pdf http://link.springer.com/article/10.1186/1472-6882-14-150/fulltext.html http://link.springer.com/content/pdf/10.1186/1472-6882-14-150 https://link.springer.com/content/pdf/10.1186/1472-6882-14-150.pdf |
genre |
Polygonum viviparum |
genre_facet |
Polygonum viviparum |
op_source |
BMC Complementary and Alternative Medicine volume 14, issue 1 ISSN 1472-6882 |
op_rights |
http://www.springer.com/tdm |
op_doi |
https://doi.org/10.1186/1472-6882-14-150 |
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BMC Complementary and Alternative Medicine |
container_volume |
14 |
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1 |
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1766173723664056320 |