Vaginal microbiome in women from Greenland assessed by microscopy and quantitative PCR

Abstract Background Bacterial vaginosis (BV) is a common condition, although its aetiology remains unexplained. The aim of this study was to analyse the composition of vaginal microbiota in women from Greenland to provide a quantitative description and improve the understanding of BV. Methods Self-c...

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Published in:BMC Infectious Diseases
Main Authors: Datcu, Raluca, Gesink, Dionne, Mulvad, Gert, Montgomery-Andersen, Ruth, Rink, Elisabeth, Koch, Anders, Ahrens, Peter, Jensen, Jørgen Skov
Format: Article in Journal/Newspaper
Language:English
Published: Springer Science and Business Media LLC 2013
Subjects:
Online Access:http://dx.doi.org/10.1186/1471-2334-13-480
http://link.springer.com/content/pdf/10.1186/1471-2334-13-480.pdf
http://link.springer.com/article/10.1186/1471-2334-13-480/fulltext.html
http://link.springer.com/content/pdf/10.1186/1471-2334-13-480
https://link.springer.com/content/pdf/10.1186/1471-2334-13-480.pdf
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spelling crspringernat:10.1186/1471-2334-13-480 2023-05-15T16:28:33+02:00 Vaginal microbiome in women from Greenland assessed by microscopy and quantitative PCR Datcu, Raluca Gesink, Dionne Mulvad, Gert Montgomery-Andersen, Ruth Rink, Elisabeth Koch, Anders Ahrens, Peter Jensen, Jørgen Skov 2013 http://dx.doi.org/10.1186/1471-2334-13-480 http://link.springer.com/content/pdf/10.1186/1471-2334-13-480.pdf http://link.springer.com/article/10.1186/1471-2334-13-480/fulltext.html http://link.springer.com/content/pdf/10.1186/1471-2334-13-480 https://link.springer.com/content/pdf/10.1186/1471-2334-13-480.pdf en eng Springer Science and Business Media LLC http://www.springer.com/tdm BMC Infectious Diseases volume 13, issue 1 ISSN 1471-2334 Infectious Diseases journal-article 2013 crspringernat https://doi.org/10.1186/1471-2334-13-480 2022-01-04T08:01:53Z Abstract Background Bacterial vaginosis (BV) is a common condition, although its aetiology remains unexplained. The aim of this study was to analyse the composition of vaginal microbiota in women from Greenland to provide a quantitative description and improve the understanding of BV. Methods Self-collected vaginal smears and swabs were obtained from 177 women. The vaginal smears were graded for BV according to Nugent’s criteria. The vaginal swab samples were analysed by 19 quantitative PCRs (qPCRs) for selected vaginal bacteria and by PCR for four sexually transmitted infections (STIs). Results STIs were common: Mycoplasma genitalium 12%, Chlamydia trachomatis 7%, Neisseria gonorrhoeae 1%, and Trichomonas vaginalis 0.5%. BV was found in 45% of women, but was not associated with individual STIs. Seven of the 19 vaginal bacteria ( Atopobium vaginae , Prevotella spp., Gardnerella vaginalis , BVAB2, Eggerthella -like bacterium, Leptotrichia amnionii , and Megasphaera type 1) had areas under the receiver operating characteristic (ROC) curve > 85%, suggesting they are good predictors of BV according to Nugent. Prevotella spp. had the highest odds ratio for BV (OR 437; 95% CI 82–2779) in univariate analysis considering only specimens with a bacterial load above the threshold determined by ROC curve analysis as positive, as well as the highest adjusted odds ratio in multivariate logistic regression analysis (OR 4.4; 95% CI 1.4-13.5). BV could be subdivided into clusters dominated by a single or a few species together. Conclusions BV by Nugent score was highly prevalent. Two of seven key species ( Prevotella spp. and A. vaginae ) remained significantly associated with BV in a multivariate model after adjusting for other bacterial species. G. vaginalis and Prevotella spp. defined the majority of BV clusters. Article in Journal/Newspaper Greenland Springer Nature (via Crossref) Greenland BMC Infectious Diseases 13 1
institution Open Polar
collection Springer Nature (via Crossref)
op_collection_id crspringernat
language English
topic Infectious Diseases
spellingShingle Infectious Diseases
Datcu, Raluca
Gesink, Dionne
Mulvad, Gert
Montgomery-Andersen, Ruth
Rink, Elisabeth
Koch, Anders
Ahrens, Peter
Jensen, Jørgen Skov
Vaginal microbiome in women from Greenland assessed by microscopy and quantitative PCR
topic_facet Infectious Diseases
description Abstract Background Bacterial vaginosis (BV) is a common condition, although its aetiology remains unexplained. The aim of this study was to analyse the composition of vaginal microbiota in women from Greenland to provide a quantitative description and improve the understanding of BV. Methods Self-collected vaginal smears and swabs were obtained from 177 women. The vaginal smears were graded for BV according to Nugent’s criteria. The vaginal swab samples were analysed by 19 quantitative PCRs (qPCRs) for selected vaginal bacteria and by PCR for four sexually transmitted infections (STIs). Results STIs were common: Mycoplasma genitalium 12%, Chlamydia trachomatis 7%, Neisseria gonorrhoeae 1%, and Trichomonas vaginalis 0.5%. BV was found in 45% of women, but was not associated with individual STIs. Seven of the 19 vaginal bacteria ( Atopobium vaginae , Prevotella spp., Gardnerella vaginalis , BVAB2, Eggerthella -like bacterium, Leptotrichia amnionii , and Megasphaera type 1) had areas under the receiver operating characteristic (ROC) curve > 85%, suggesting they are good predictors of BV according to Nugent. Prevotella spp. had the highest odds ratio for BV (OR 437; 95% CI 82–2779) in univariate analysis considering only specimens with a bacterial load above the threshold determined by ROC curve analysis as positive, as well as the highest adjusted odds ratio in multivariate logistic regression analysis (OR 4.4; 95% CI 1.4-13.5). BV could be subdivided into clusters dominated by a single or a few species together. Conclusions BV by Nugent score was highly prevalent. Two of seven key species ( Prevotella spp. and A. vaginae ) remained significantly associated with BV in a multivariate model after adjusting for other bacterial species. G. vaginalis and Prevotella spp. defined the majority of BV clusters.
format Article in Journal/Newspaper
author Datcu, Raluca
Gesink, Dionne
Mulvad, Gert
Montgomery-Andersen, Ruth
Rink, Elisabeth
Koch, Anders
Ahrens, Peter
Jensen, Jørgen Skov
author_facet Datcu, Raluca
Gesink, Dionne
Mulvad, Gert
Montgomery-Andersen, Ruth
Rink, Elisabeth
Koch, Anders
Ahrens, Peter
Jensen, Jørgen Skov
author_sort Datcu, Raluca
title Vaginal microbiome in women from Greenland assessed by microscopy and quantitative PCR
title_short Vaginal microbiome in women from Greenland assessed by microscopy and quantitative PCR
title_full Vaginal microbiome in women from Greenland assessed by microscopy and quantitative PCR
title_fullStr Vaginal microbiome in women from Greenland assessed by microscopy and quantitative PCR
title_full_unstemmed Vaginal microbiome in women from Greenland assessed by microscopy and quantitative PCR
title_sort vaginal microbiome in women from greenland assessed by microscopy and quantitative pcr
publisher Springer Science and Business Media LLC
publishDate 2013
url http://dx.doi.org/10.1186/1471-2334-13-480
http://link.springer.com/content/pdf/10.1186/1471-2334-13-480.pdf
http://link.springer.com/article/10.1186/1471-2334-13-480/fulltext.html
http://link.springer.com/content/pdf/10.1186/1471-2334-13-480
https://link.springer.com/content/pdf/10.1186/1471-2334-13-480.pdf
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