Screening and analysis of PoAkirin1 and two related genes in response to immunological stimulants in the Japanese flounder (Paralichthys olivaceus)
Abstract A member of the NF-κB signaling pathway, PoAkirin1, was cloned from a full-length cDNA library of Japanese flounder ( Paralichthys olivaceus ). The full-length cDNA comprises a 5′UTR of 202 bp, an open reading frame of 564 bp encoding a 187-amino-acid polypeptide and a 521-bp 3′UTR with a p...
Published in: | BMC Molecular Biology |
---|---|
Main Authors: | , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Springer Science and Business Media LLC
2013
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1186/1471-2199-14-10 https://link.springer.com/content/pdf/10.1186/1471-2199-14-10.pdf |
id |
crspringernat:10.1186/1471-2199-14-10 |
---|---|
record_format |
openpolar |
spelling |
crspringernat:10.1186/1471-2199-14-10 2023-05-15T18:15:49+02:00 Screening and analysis of PoAkirin1 and two related genes in response to immunological stimulants in the Japanese flounder (Paralichthys olivaceus) Yang, Chang-Geng Wang, Xian-Li Zhang, Bo Sun, Bing Liu, Shan-Shan Chen, Song-Lin 2013 http://dx.doi.org/10.1186/1471-2199-14-10 https://link.springer.com/content/pdf/10.1186/1471-2199-14-10.pdf en eng Springer Science and Business Media LLC BMC Molecular Biology volume 14, issue 1 ISSN 1471-2199 Molecular Biology journal-article 2013 crspringernat https://doi.org/10.1186/1471-2199-14-10 2022-01-04T13:03:12Z Abstract A member of the NF-κB signaling pathway, PoAkirin1, was cloned from a full-length cDNA library of Japanese flounder ( Paralichthys olivaceus ). The full-length cDNA comprises a 5′UTR of 202 bp, an open reading frame of 564 bp encoding a 187-amino-acid polypeptide and a 521-bp 3′UTR with a poly (A) tail. The putative protein has a predicted molecular mass of 21 kDa and an isoelectric point (pI) of 9.22. Amino acid sequence alignments showed that PoAkirin1 was 99% identical to the Scophthalmus maximus Akirin protein (ADK27484). Yeast two-hybrid assays identified two proteins that interact with PoAkirin1: PoHEPN and PoC1q. The cDNA sequences of PoHEPN and PoC1q are 672 bp and 528 bp, respectively. Real-time quantitative reverse-transcriptase polymerase chain reaction analysis showed that bacteria could induce the expressions of PoAkirin1, PoHEPN and PoC1q. However, the responses of PoHEPN and PoC1q to the bacterial challenge were slower than that of PoAkirin1. To further study the function of PoAkirin1, recombinant PoAkirin1 and PoHEPN were expressed in Escherichia coli and would be used to verify the PoAkirin1 - PoHEPN binding activity. These results identified two proteins that potentially interact with PoAkirin1 and that bacteria could induce their expression. Article in Journal/Newspaper Scophthalmus maximus Springer Nature (via Crossref) BMC Molecular Biology 14 1 |
institution |
Open Polar |
collection |
Springer Nature (via Crossref) |
op_collection_id |
crspringernat |
language |
English |
topic |
Molecular Biology |
spellingShingle |
Molecular Biology Yang, Chang-Geng Wang, Xian-Li Zhang, Bo Sun, Bing Liu, Shan-Shan Chen, Song-Lin Screening and analysis of PoAkirin1 and two related genes in response to immunological stimulants in the Japanese flounder (Paralichthys olivaceus) |
topic_facet |
Molecular Biology |
description |
Abstract A member of the NF-κB signaling pathway, PoAkirin1, was cloned from a full-length cDNA library of Japanese flounder ( Paralichthys olivaceus ). The full-length cDNA comprises a 5′UTR of 202 bp, an open reading frame of 564 bp encoding a 187-amino-acid polypeptide and a 521-bp 3′UTR with a poly (A) tail. The putative protein has a predicted molecular mass of 21 kDa and an isoelectric point (pI) of 9.22. Amino acid sequence alignments showed that PoAkirin1 was 99% identical to the Scophthalmus maximus Akirin protein (ADK27484). Yeast two-hybrid assays identified two proteins that interact with PoAkirin1: PoHEPN and PoC1q. The cDNA sequences of PoHEPN and PoC1q are 672 bp and 528 bp, respectively. Real-time quantitative reverse-transcriptase polymerase chain reaction analysis showed that bacteria could induce the expressions of PoAkirin1, PoHEPN and PoC1q. However, the responses of PoHEPN and PoC1q to the bacterial challenge were slower than that of PoAkirin1. To further study the function of PoAkirin1, recombinant PoAkirin1 and PoHEPN were expressed in Escherichia coli and would be used to verify the PoAkirin1 - PoHEPN binding activity. These results identified two proteins that potentially interact with PoAkirin1 and that bacteria could induce their expression. |
format |
Article in Journal/Newspaper |
author |
Yang, Chang-Geng Wang, Xian-Li Zhang, Bo Sun, Bing Liu, Shan-Shan Chen, Song-Lin |
author_facet |
Yang, Chang-Geng Wang, Xian-Li Zhang, Bo Sun, Bing Liu, Shan-Shan Chen, Song-Lin |
author_sort |
Yang, Chang-Geng |
title |
Screening and analysis of PoAkirin1 and two related genes in response to immunological stimulants in the Japanese flounder (Paralichthys olivaceus) |
title_short |
Screening and analysis of PoAkirin1 and two related genes in response to immunological stimulants in the Japanese flounder (Paralichthys olivaceus) |
title_full |
Screening and analysis of PoAkirin1 and two related genes in response to immunological stimulants in the Japanese flounder (Paralichthys olivaceus) |
title_fullStr |
Screening and analysis of PoAkirin1 and two related genes in response to immunological stimulants in the Japanese flounder (Paralichthys olivaceus) |
title_full_unstemmed |
Screening and analysis of PoAkirin1 and two related genes in response to immunological stimulants in the Japanese flounder (Paralichthys olivaceus) |
title_sort |
screening and analysis of poakirin1 and two related genes in response to immunological stimulants in the japanese flounder (paralichthys olivaceus) |
publisher |
Springer Science and Business Media LLC |
publishDate |
2013 |
url |
http://dx.doi.org/10.1186/1471-2199-14-10 https://link.springer.com/content/pdf/10.1186/1471-2199-14-10.pdf |
genre |
Scophthalmus maximus |
genre_facet |
Scophthalmus maximus |
op_source |
BMC Molecular Biology volume 14, issue 1 ISSN 1471-2199 |
op_doi |
https://doi.org/10.1186/1471-2199-14-10 |
container_title |
BMC Molecular Biology |
container_volume |
14 |
container_issue |
1 |
_version_ |
1766189051943059456 |