MLVA-16 typing of 295 marine mammal Brucella isolates from different animal and geographic origins identifies 7 major groups within Brucella ceti and Brucella pinnipedialis
Abstract Background Since 1994, Brucella strains have been isolated from a wide range of marine mammals. They are currently recognized as two new Brucella species, B. pinnipedialis for the pinniped isolates and B. ceti for the cetacean isolates in agreement with host preference and specific phenotyp...
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2009
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Online Access: | http://dx.doi.org/10.1186/1471-2180-9-145 https://link.springer.com/content/pdf/10.1186/1471-2180-9-145.pdf |
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crspringernat:10.1186/1471-2180-9-145 2023-05-15T15:59:54+02:00 MLVA-16 typing of 295 marine mammal Brucella isolates from different animal and geographic origins identifies 7 major groups within Brucella ceti and Brucella pinnipedialis Maquart, Marianne Le Flèche, Philippe Foster, Geoffrey Tryland, Morten Ramisse, Françoise Djønne, Berit Al Dahouk, Sascha Jacques, Isabelle Neubauer, Heinrich Walravens, Karl Godfroid, Jacques Cloeckaert, Axel Vergnaud, Gilles 2009 http://dx.doi.org/10.1186/1471-2180-9-145 https://link.springer.com/content/pdf/10.1186/1471-2180-9-145.pdf en eng Springer Science and Business Media LLC BMC Microbiology volume 9, issue 1 ISSN 1471-2180 Microbiology (medical) Microbiology journal-article 2009 crspringernat https://doi.org/10.1186/1471-2180-9-145 2022-01-04T15:38:45Z Abstract Background Since 1994, Brucella strains have been isolated from a wide range of marine mammals. They are currently recognized as two new Brucella species, B. pinnipedialis for the pinniped isolates and B. ceti for the cetacean isolates in agreement with host preference and specific phenotypic and molecular markers. In order to investigate the genetic relationships within the marine mammal Brucella isolates and with reference to terrestrial mammal Brucella isolates, we applied in this study the Multiple Loci VNTR (Variable Number of Tandem Repeats) Analysis (MLVA) approach. A previously published assay comprising 16 loci (MLVA-16) that has been shown to be highly relevant and efficient for typing and clustering Brucella strains from animal and human origin was used. Results 294 marine mammal Brucella strains collected in European waters from 173 animals and a human isolate from New Zealand presumably from marine origin were investigated by MLVA-16. Marine mammal Brucella isolates were shown to be different from the recognized terrestrial mammal Brucella species and biovars and corresponded to 3 major related groups, one specific of the B. ceti strains, one of the B. pinnipedialis strains and the last composed of the human isolate. In the B. ceti group, 3 subclusters were identified, distinguishing a cluster of dolphin, minke whale and porpoise isolates and two clusters mostly composed of dolphin isolates. These results were in accordance with published analyses using other phenotypic or molecular approaches, or different panels of VNTR loci. The B. pinnipedialis group could be similarly subdivided in 3 subclusters, one composed exclusively of isolates from hooded seals ( Cystophora cristata ) and the two others comprising other seal species isolates. Conclusion The clustering analysis of a large collection of marine mammal Brucella isolates from European waters significantly strengthens the current view of the population structure of these two species, and their relative position with respect to the rest of the Brucella genus. MLVA-16 is confirmed as being a rapid, highly discriminatory and reproducible method to classify Brucella strains including the marine mammal isolates. The Brucella2009 MLVA-16 genotyping database available at http://mlva.u-psud.fr/ is providing a detailed coverage of all 9 currently recognized Brucella species. Article in Journal/Newspaper Cystophora cristata minke whale Springer Nature (via Crossref) New Zealand BMC Microbiology 9 1 |
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Open Polar |
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Springer Nature (via Crossref) |
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crspringernat |
language |
English |
topic |
Microbiology (medical) Microbiology |
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Microbiology (medical) Microbiology Maquart, Marianne Le Flèche, Philippe Foster, Geoffrey Tryland, Morten Ramisse, Françoise Djønne, Berit Al Dahouk, Sascha Jacques, Isabelle Neubauer, Heinrich Walravens, Karl Godfroid, Jacques Cloeckaert, Axel Vergnaud, Gilles MLVA-16 typing of 295 marine mammal Brucella isolates from different animal and geographic origins identifies 7 major groups within Brucella ceti and Brucella pinnipedialis |
topic_facet |
Microbiology (medical) Microbiology |
description |
Abstract Background Since 1994, Brucella strains have been isolated from a wide range of marine mammals. They are currently recognized as two new Brucella species, B. pinnipedialis for the pinniped isolates and B. ceti for the cetacean isolates in agreement with host preference and specific phenotypic and molecular markers. In order to investigate the genetic relationships within the marine mammal Brucella isolates and with reference to terrestrial mammal Brucella isolates, we applied in this study the Multiple Loci VNTR (Variable Number of Tandem Repeats) Analysis (MLVA) approach. A previously published assay comprising 16 loci (MLVA-16) that has been shown to be highly relevant and efficient for typing and clustering Brucella strains from animal and human origin was used. Results 294 marine mammal Brucella strains collected in European waters from 173 animals and a human isolate from New Zealand presumably from marine origin were investigated by MLVA-16. Marine mammal Brucella isolates were shown to be different from the recognized terrestrial mammal Brucella species and biovars and corresponded to 3 major related groups, one specific of the B. ceti strains, one of the B. pinnipedialis strains and the last composed of the human isolate. In the B. ceti group, 3 subclusters were identified, distinguishing a cluster of dolphin, minke whale and porpoise isolates and two clusters mostly composed of dolphin isolates. These results were in accordance with published analyses using other phenotypic or molecular approaches, or different panels of VNTR loci. The B. pinnipedialis group could be similarly subdivided in 3 subclusters, one composed exclusively of isolates from hooded seals ( Cystophora cristata ) and the two others comprising other seal species isolates. Conclusion The clustering analysis of a large collection of marine mammal Brucella isolates from European waters significantly strengthens the current view of the population structure of these two species, and their relative position with respect to the rest of the Brucella genus. MLVA-16 is confirmed as being a rapid, highly discriminatory and reproducible method to classify Brucella strains including the marine mammal isolates. The Brucella2009 MLVA-16 genotyping database available at http://mlva.u-psud.fr/ is providing a detailed coverage of all 9 currently recognized Brucella species. |
format |
Article in Journal/Newspaper |
author |
Maquart, Marianne Le Flèche, Philippe Foster, Geoffrey Tryland, Morten Ramisse, Françoise Djønne, Berit Al Dahouk, Sascha Jacques, Isabelle Neubauer, Heinrich Walravens, Karl Godfroid, Jacques Cloeckaert, Axel Vergnaud, Gilles |
author_facet |
Maquart, Marianne Le Flèche, Philippe Foster, Geoffrey Tryland, Morten Ramisse, Françoise Djønne, Berit Al Dahouk, Sascha Jacques, Isabelle Neubauer, Heinrich Walravens, Karl Godfroid, Jacques Cloeckaert, Axel Vergnaud, Gilles |
author_sort |
Maquart, Marianne |
title |
MLVA-16 typing of 295 marine mammal Brucella isolates from different animal and geographic origins identifies 7 major groups within Brucella ceti and Brucella pinnipedialis |
title_short |
MLVA-16 typing of 295 marine mammal Brucella isolates from different animal and geographic origins identifies 7 major groups within Brucella ceti and Brucella pinnipedialis |
title_full |
MLVA-16 typing of 295 marine mammal Brucella isolates from different animal and geographic origins identifies 7 major groups within Brucella ceti and Brucella pinnipedialis |
title_fullStr |
MLVA-16 typing of 295 marine mammal Brucella isolates from different animal and geographic origins identifies 7 major groups within Brucella ceti and Brucella pinnipedialis |
title_full_unstemmed |
MLVA-16 typing of 295 marine mammal Brucella isolates from different animal and geographic origins identifies 7 major groups within Brucella ceti and Brucella pinnipedialis |
title_sort |
mlva-16 typing of 295 marine mammal brucella isolates from different animal and geographic origins identifies 7 major groups within brucella ceti and brucella pinnipedialis |
publisher |
Springer Science and Business Media LLC |
publishDate |
2009 |
url |
http://dx.doi.org/10.1186/1471-2180-9-145 https://link.springer.com/content/pdf/10.1186/1471-2180-9-145.pdf |
geographic |
New Zealand |
geographic_facet |
New Zealand |
genre |
Cystophora cristata minke whale |
genre_facet |
Cystophora cristata minke whale |
op_source |
BMC Microbiology volume 9, issue 1 ISSN 1471-2180 |
op_doi |
https://doi.org/10.1186/1471-2180-9-145 |
container_title |
BMC Microbiology |
container_volume |
9 |
container_issue |
1 |
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1766395799066902528 |