Inflammatory responses in primary muscle cell cultures in Atlantic salmon (Salmo salar)
Abstract Background The relationship between fish health and muscle growth is critical for continued expansion of the aquaculture industry. The effect of immune stimulation on the expression of genes related to the energy balance of fish is poorly understood. In mammals immune stimulation results in...
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crspringernat:10.1186/1471-2164-14-747 2023-05-15T15:31:07+02:00 Inflammatory responses in primary muscle cell cultures in Atlantic salmon (Salmo salar) Pooley, Nicholas J Tacchi, Luca Secombes, Christopher J Martin, Samuel AM 2013 http://dx.doi.org/10.1186/1471-2164-14-747 https://link.springer.com/content/pdf/10.1186/1471-2164-14-747.pdf en eng Springer Science and Business Media LLC BMC Genomics volume 14, issue 1 ISSN 1471-2164 Genetics Biotechnology journal-article 2013 crspringernat https://doi.org/10.1186/1471-2164-14-747 2022-01-04T11:18:03Z Abstract Background The relationship between fish health and muscle growth is critical for continued expansion of the aquaculture industry. The effect of immune stimulation on the expression of genes related to the energy balance of fish is poorly understood. In mammals immune stimulation results in major transcriptional changes in muscle, potentially to allow a reallocation of amino acids for use in the immune response and energy homeostasis. The aim of this study was to investigate the effects of immune stimulation on fish muscle gene expression. Results Atlantic salmon ( Salmo salar ) primary muscle cell cultures were stimulated with recombinant (r)IL-1β, a major proinflammatory cytokine, for 24 h in order to simulate an acute immune response. The transcriptomic response was determined by RNA hybridization to a 4 × 44 K Agilent Atlantic salmon microarray platform. The rIL-1β stimulation induced the expression of genes related to both the innate and adaptive immune systems. In addition there were highly significant changes in the expression of genes related to regulation of the cell cycle, growth/structural proteins, proteolysis and lipid metabolism. Of interest were a number of IGF binding proteins that were differentially expressed, which may demonstrate cross talk between the growth and immune systems. Conclusion We show rIL-1β modulates the expression of not only immune related genes, but also that of genes involved in processes related to growth and metabolism. Co-stimulation of muscle cells with both rIGF-I and rIL-1β demonstrates cross talk between these pathways providing potential avenues for further research. This study highlights the potential negative effects of inflammation on muscle protein deposition and growth in fish and extends our understanding of energy allocation in ectothermic animals. Article in Journal/Newspaper Atlantic salmon Salmo salar Springer Nature (via Crossref) BMC Genomics 14 1 747 |
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Genetics Biotechnology |
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Genetics Biotechnology Pooley, Nicholas J Tacchi, Luca Secombes, Christopher J Martin, Samuel AM Inflammatory responses in primary muscle cell cultures in Atlantic salmon (Salmo salar) |
topic_facet |
Genetics Biotechnology |
description |
Abstract Background The relationship between fish health and muscle growth is critical for continued expansion of the aquaculture industry. The effect of immune stimulation on the expression of genes related to the energy balance of fish is poorly understood. In mammals immune stimulation results in major transcriptional changes in muscle, potentially to allow a reallocation of amino acids for use in the immune response and energy homeostasis. The aim of this study was to investigate the effects of immune stimulation on fish muscle gene expression. Results Atlantic salmon ( Salmo salar ) primary muscle cell cultures were stimulated with recombinant (r)IL-1β, a major proinflammatory cytokine, for 24 h in order to simulate an acute immune response. The transcriptomic response was determined by RNA hybridization to a 4 × 44 K Agilent Atlantic salmon microarray platform. The rIL-1β stimulation induced the expression of genes related to both the innate and adaptive immune systems. In addition there were highly significant changes in the expression of genes related to regulation of the cell cycle, growth/structural proteins, proteolysis and lipid metabolism. Of interest were a number of IGF binding proteins that were differentially expressed, which may demonstrate cross talk between the growth and immune systems. Conclusion We show rIL-1β modulates the expression of not only immune related genes, but also that of genes involved in processes related to growth and metabolism. Co-stimulation of muscle cells with both rIGF-I and rIL-1β demonstrates cross talk between these pathways providing potential avenues for further research. This study highlights the potential negative effects of inflammation on muscle protein deposition and growth in fish and extends our understanding of energy allocation in ectothermic animals. |
format |
Article in Journal/Newspaper |
author |
Pooley, Nicholas J Tacchi, Luca Secombes, Christopher J Martin, Samuel AM |
author_facet |
Pooley, Nicholas J Tacchi, Luca Secombes, Christopher J Martin, Samuel AM |
author_sort |
Pooley, Nicholas J |
title |
Inflammatory responses in primary muscle cell cultures in Atlantic salmon (Salmo salar) |
title_short |
Inflammatory responses in primary muscle cell cultures in Atlantic salmon (Salmo salar) |
title_full |
Inflammatory responses in primary muscle cell cultures in Atlantic salmon (Salmo salar) |
title_fullStr |
Inflammatory responses in primary muscle cell cultures in Atlantic salmon (Salmo salar) |
title_full_unstemmed |
Inflammatory responses in primary muscle cell cultures in Atlantic salmon (Salmo salar) |
title_sort |
inflammatory responses in primary muscle cell cultures in atlantic salmon (salmo salar) |
publisher |
Springer Science and Business Media LLC |
publishDate |
2013 |
url |
http://dx.doi.org/10.1186/1471-2164-14-747 https://link.springer.com/content/pdf/10.1186/1471-2164-14-747.pdf |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
BMC Genomics volume 14, issue 1 ISSN 1471-2164 |
op_doi |
https://doi.org/10.1186/1471-2164-14-747 |
container_title |
BMC Genomics |
container_volume |
14 |
container_issue |
1 |
container_start_page |
747 |
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1766361614110425088 |