Expression profiling reveals Spot 42 small RNA as a key regulator in the central metabolism of Aliivibrio salmonicida
Abstract Background Spot 42 was discovered in Escherichia coli nearly 40 years ago as an abundant, small and unstable RNA. Its biological role has remained obscure until recently, and is today implicated in having broader roles in the central and secondary metabolism. Spot 42 is encoded by the spf g...
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crspringernat:10.1186/1471-2164-13-37 2023-05-15T15:32:44+02:00 Expression profiling reveals Spot 42 small RNA as a key regulator in the central metabolism of Aliivibrio salmonicida Hansen, Geir Å Ahmad, Rafi Hjerde, Erik Fenton, Christopher G Willassen, Nils-Peder Haugen, Peik 2012 http://dx.doi.org/10.1186/1471-2164-13-37 https://link.springer.com/content/pdf/10.1186/1471-2164-13-37.pdf en eng Springer Science and Business Media LLC BMC Genomics volume 13, issue 1 ISSN 1471-2164 Genetics Biotechnology journal-article 2012 crspringernat https://doi.org/10.1186/1471-2164-13-37 2022-01-04T10:53:10Z Abstract Background Spot 42 was discovered in Escherichia coli nearly 40 years ago as an abundant, small and unstable RNA. Its biological role has remained obscure until recently, and is today implicated in having broader roles in the central and secondary metabolism. Spot 42 is encoded by the spf gene. The gene is ubiquitous in the Vibrionaceae family of gamma-proteobacteria. One member of this family, Aliivibrio salmonicida , causes cold-water vibriosis in farmed Atlantic salmon. Its genome encodes Spot 42 with 84% identity to E. coli Spot 42. Results We generated a A. salmonicida spf deletion mutant. We then used microarray and Northern blot analyses to monitor global effects on the transcriptome in order to provide insights into the biological roles of Spot 42 in this bacterium. In the presence of glucose, we found a surprisingly large number of ≥ 2X differentially expressed genes, and several major cellular processes were affected. A gene encoding a pirin-like protein showed an on/off expression pattern in the presence/absence of Spot 42, which suggests that Spot 42 plays a key regulatory role in the central metabolism by regulating the switch between fermentation and respiration. Interestingly, we discovered an sRNA named VSsrna24, which is encoded immediately downstream of spf . This new sRNA has an expression pattern opposite to that of Spot 42, and its expression is repressed by glucose. Conclusions We hypothesize that Spot 42 plays a key role in the central metabolism, in part by regulating the pyruvat dehydrogenase enzyme complex via pirin. Article in Journal/Newspaper Atlantic salmon Springer Nature (via Crossref) BMC Genomics 13 1 |
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Springer Nature (via Crossref) |
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English |
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Genetics Biotechnology |
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Genetics Biotechnology Hansen, Geir Å Ahmad, Rafi Hjerde, Erik Fenton, Christopher G Willassen, Nils-Peder Haugen, Peik Expression profiling reveals Spot 42 small RNA as a key regulator in the central metabolism of Aliivibrio salmonicida |
topic_facet |
Genetics Biotechnology |
description |
Abstract Background Spot 42 was discovered in Escherichia coli nearly 40 years ago as an abundant, small and unstable RNA. Its biological role has remained obscure until recently, and is today implicated in having broader roles in the central and secondary metabolism. Spot 42 is encoded by the spf gene. The gene is ubiquitous in the Vibrionaceae family of gamma-proteobacteria. One member of this family, Aliivibrio salmonicida , causes cold-water vibriosis in farmed Atlantic salmon. Its genome encodes Spot 42 with 84% identity to E. coli Spot 42. Results We generated a A. salmonicida spf deletion mutant. We then used microarray and Northern blot analyses to monitor global effects on the transcriptome in order to provide insights into the biological roles of Spot 42 in this bacterium. In the presence of glucose, we found a surprisingly large number of ≥ 2X differentially expressed genes, and several major cellular processes were affected. A gene encoding a pirin-like protein showed an on/off expression pattern in the presence/absence of Spot 42, which suggests that Spot 42 plays a key regulatory role in the central metabolism by regulating the switch between fermentation and respiration. Interestingly, we discovered an sRNA named VSsrna24, which is encoded immediately downstream of spf . This new sRNA has an expression pattern opposite to that of Spot 42, and its expression is repressed by glucose. Conclusions We hypothesize that Spot 42 plays a key role in the central metabolism, in part by regulating the pyruvat dehydrogenase enzyme complex via pirin. |
format |
Article in Journal/Newspaper |
author |
Hansen, Geir Å Ahmad, Rafi Hjerde, Erik Fenton, Christopher G Willassen, Nils-Peder Haugen, Peik |
author_facet |
Hansen, Geir Å Ahmad, Rafi Hjerde, Erik Fenton, Christopher G Willassen, Nils-Peder Haugen, Peik |
author_sort |
Hansen, Geir Å |
title |
Expression profiling reveals Spot 42 small RNA as a key regulator in the central metabolism of Aliivibrio salmonicida |
title_short |
Expression profiling reveals Spot 42 small RNA as a key regulator in the central metabolism of Aliivibrio salmonicida |
title_full |
Expression profiling reveals Spot 42 small RNA as a key regulator in the central metabolism of Aliivibrio salmonicida |
title_fullStr |
Expression profiling reveals Spot 42 small RNA as a key regulator in the central metabolism of Aliivibrio salmonicida |
title_full_unstemmed |
Expression profiling reveals Spot 42 small RNA as a key regulator in the central metabolism of Aliivibrio salmonicida |
title_sort |
expression profiling reveals spot 42 small rna as a key regulator in the central metabolism of aliivibrio salmonicida |
publisher |
Springer Science and Business Media LLC |
publishDate |
2012 |
url |
http://dx.doi.org/10.1186/1471-2164-13-37 https://link.springer.com/content/pdf/10.1186/1471-2164-13-37.pdf |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
BMC Genomics volume 13, issue 1 ISSN 1471-2164 |
op_doi |
https://doi.org/10.1186/1471-2164-13-37 |
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BMC Genomics |
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13 |
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1 |
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1766363221994766336 |